Light Intensity-Induced Morphogenetic Response and Enhanced β-Sitosterol Accumulation in Date Palm (Phoenix dactylifera L. cv. Hayani) Callus Culture

El-Dawayati, Maiada M.; El-Sharabasy, Sherif F.; Gantait, Saikat

doi:10.1007/s12355-020-00844-9

Cited by 10 publications

(6 citation statements)

References 32 publications

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“…For example, in callus cultures of Galega officinalis , low temperature and UV- C irradiation improved secondary metabolite accumulation, while higher temperatures negatively impacted the phenolic acid levels [ 71 ]. Different light intensities noticeably stimulated the biosynthesis of secondary metabolites in cell cultures of A. absinthium and P. dactylifera [ 57 , 59 ], but this effect was not seen in our experiments with sweet potato cell culture.…”

Section: Resultsmentioning

confidence: 74%

“…Light is a crucial factor for plant growth and differentiation, capable of causing morphogenetic responses in cultured cells [ 57 ]. However, the sudden increase in light intensity, i.e., high light stress, leads to excessive reactive oxygen species (ROS) production and cell damage [ 58 ].…”

Section: Resultsmentioning

confidence: 99%

“…Likewise, light reduced biomass accumulation of O. sativa cell culture [ 54 ]. Light conditions resulted in necrosis or browning in Phoenix dactylifera callus culture and this effect corresponded with the illumination intensity [ 57 ].…”

Section: Resultsmentioning

confidence: 99%

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Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas

Vasyutkina

Yugay

Grigorchuk

et al. 2022

IJMS

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Ipomoea batatas is a vital root crop and a source of caffeoylquinic acid derivatives (CQAs) with potential health-promoting benefits. As a naturally transgenic plant, I. batatas contains cellular T-DNA (cT-DNA) sequence homologs of the Agrobacterium rhizogenes open reading frame (ORF)14, ORF17n, rooting locus (Rol)B/RolC, ORF13, and ORF18/ORF17n of unknown function. This study aimed to evaluate the effect of abiotic stresses (temperature, ultraviolet, and light) and chemical elicitors (methyl jasmonate, salicylic acid, and sodium nitroprusside) on the biosynthesis of CQAs and cT-DNA gene expression in I. batatas cell culture as a model system. Among all the applied treatments, ultraviolet irradiation, methyl jasmonate, and salicylic acid caused the maximal accumulation of secondary compounds. We also discovered that I. batatas cT-DNA genes were not expressed in cell culture, and the studied conditions weakly affected their transcriptional levels. However, the Ib-rolB/C gene expressed under the strong 35S CaMV promoter increased the CQAs content by 1.5–1.9-fold. Overall, our results show that cT-DNA-encoded transgenes are not involved in stress- and chemical elicitor-induced CQAs accumulation in cell cultures of I. batatas. Nevertheless, overaccumulation of RolB/RolC transcripts potentiates the secondary metabolism of sweet potatoes through a currently unknown mechanism. Our study provides new insights into the molecular mechanisms linked with CQAs biosynthesis in cell culture of naturally transgenic food crops, i.e., sweet potato.

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Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

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Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas

Vasyutkina

Yugay

Grigorchuk

et al. 2022

IJMS

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“…Light irradiation is one of the physical factors that influence the growth and development of plant tissues as well as the biosynthesis of various phytomolecules (El-Dawayati et al, 2020 andYoussef et al, 2021). Among the widely used tools of biotechnology, elicitation is an important strategy that stimulates the production of highvalue compounds in medicinal crops.…”

Section: Plantmentioning

confidence: 99%

Effect of chitosan and light conditions on the production of callus biomass, total flavonoids and total phenolics in Ginkgo biloba L.

Elateeq

Saad

Eissa

et al. 2021

Al-Azhar Journal of Agricultural Research

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Ginkgo biloba L. is a well-known tree for its high medicinal value owing to the presence of terpene trilactones, unique active metabolites, and other phytochemicals, including flavonoids and phenolics in its leaves. In Egypt, G. biloba has been reported as one of the endangered trees. Hence, plant tissue technique could be a potential alternative system to produce ginkgo's pharmaceutical compounds. In this study, an elicitation strategy using the biotic elicitor chitosan was employed to enhance the productivity of certain metabolites in G. biloba leaf-derived callus incubated in light (16 h photoperiod) or darkness. Combination of 1.0 mg/L α-naphthalene acetic acid (NAA) and 2.0 mg/L 6-benzyl adenine (BA) added to Murashige and Skoog (MS) medium resulted in the highest callogenesis percentage in both light and dark (95.00 and 94.43%, respectively) with a compacted callus texture but a different color. Significant differences were recorded between chitosan levels concerning callus biomass production and total flavonoids and phenolics accumulation. The highest yield of callus fresh and dry weights, growth index, and relative growth rate were recorded for light-grown cultures elicited with 100 mg/L chitosan followed by 50 mg/L. Same concentrations were also superior in enhancing the content and productivity of total flavonoids and total phenolics. Light incubation was more favorable than darkness for callus growth, flavonoid, and phenolic biosynthesis as well as antioxidant activity. The current study revealed the possibility of using calli culture as a biotechnological approach for the industrial production of G. biloba phytoconstituents.

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“…The light environment has important effects on the morphology, physiology, and quality of plant seedlings propagated in vitro [18][19][20][21][22][23] . Under high light conditions (42 µmol/m 2 /s PPFD), the volume of calli was signi cantly increased in Phoenix dactylifera 24 ; light duration in uences leaf color, seedling height growth, and rooting speed of tissue culture seedlings of Catalpa 25 . Plants have different responses to the same type of light, and a high proportion of BL (30%) can induce stress and growth inhibition or improve growth and development, depending on the species 26 .…”

Section: Introductionmentioning

confidence: 96%

Effects of Light Treatments on Callus Induction and Morphogenesis in Caladium bicolor

Chen,

Xie,

Guan

et al. 2023

Preprint

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Caladium bicolor is widely used as an ornamental plant outdoors and indoors due to its rich colors, diverse combinations, and strange patterns. In the commercial production of Caladium bicolor, tissue culture technology can quickly reproduce seedlings with consistent genetic properties. In practice, there are still aspects that can be improved in terms of energy dissipation and ornamental value when using fluorescent lamps. In this experiment, the light intensity had no significant effect on the induction of callus and the germination rate, but from the number of buds per explant increased. Different light waves affect the occurrence of seedlings of Caladium bicolor. Among them, a single light source, LED-Red, is not conducive to callus differentiation, plant height, and carotenoid accumulation compared to other treatments; LED-Blue is beneficial for the formation of relative anthocyanin content and plant height.LED-White is beneficial for leaf size. Fluorescence is not conducive to increasing the total number of seedlings, which is important for production. Compared with fluorescence, the transplanting rate of LED-Blue increased by 94.92%. The results of this experiment suggest that LED light can replace florescent lamps in tissue culture for achieving low energy consumption and high efficiency.

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Light Intensity-Induced Morphogenetic Response and Enhanced β-Sitosterol Accumulation in Date Palm (Phoenix dactylifera L. cv. Hayani) Callus Culture

Cited by 10 publications

References 32 publications

Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas

Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatas

Effect of chitosan and light conditions on the production of callus biomass, total flavonoids and total phenolics in Ginkgo biloba L.

Effects of Light Treatments on Callus Induction and Morphogenesis in Caladium bicolor

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