Light Quality Affects Protocorm-Like Body (PLB) Formation, Growth and Development of In Vitro Plantlets of Phalaenopsis pulcherrima

Chashmi, Kobra Ahmadi; Omran, Vali Ollah Ghasemi; Ebrahimi, Raheleh; Moradi, Hosein; Abdosi, Vahid

doi:10.1134/s1062359022050107

Cited by 4 publications

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shoot elongation, shoot number, and chlorophyll a and b content were promoted in response to R: B LEDs. [126] Rhynchostylis retusa (L.) Blume Root tips…”

Section: Paphiopedilum Insigne Seedsmentioning

confidence: 99%

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

View full text Add to dashboard Cite

Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation.

show abstract

“…Shoot elongation, shoot number, and chlorophyll a and b content were promoted in response to R: B LEDs. [126] Rhynchostylis retusa (L.) Blume Root tips…”

Section: Paphiopedilum Insigne Seedsmentioning

confidence: 99%

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

Protocorm-Like Body Induction and Development Using In Vitro Culture Techniques and Biotechnological Methods

Yeung,

Yeung

2024

Springer Protocols Handbooks

View full text Add to dashboard Cite

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Rojas-Vargas

Castander-Olarieta²,

Nascimento³

et al. 2023

Plants

View full text Add to dashboard Cite

Pinus. ponderosa (P. Lawson and C. Lawson) is a commercial tree and one of the most important forest species in North America. Ponderosa pine suffers hardship when going through vegetative propagation and, in some cases, 15–30 years are needed to achieve full reproductive capacity. Based on previous works on P. ponderosa regeneration through in vitro organogenesis and trying to improve the published protocols, our objective was to analyze the influence of different types of explants, basal culture media, cytokinins, auxins, and light treatments on the success of shoot multiplication and rooting phases. Whole zygotic embryos and 44 µΜ 6-benzyladenine showed the best results in terms of explants survival. For shoot organogenesis, whole zygotic embryos and half LP (LP medium, Quoirin and Lepoivre, 1977, modified by Aitken-Christie et al., 1988) macronutrients were selected. A significant positive interaction between whole zygotic embryos and half LP macronutrients was found for the percentage of explants forming shoots. Regarding the light treatments applied, a significantly higher percentage of shoots elongated enough to be rooted was detected in shoots growing under blue LED at a light intensity of 61.09 µmol m−2 s−1. However, the acclimatization percentage was higher in shoots previously cultivated under fluorescent light at a light intensity of 61.71 µmol m−2 s−1. Anatomical studies using light microscopy and scanning electron microscopy showed the light treatments promoted differences in anatomical aspects in in vitro shoots; needles of plantlets exposed to red and blue LEDs revealed less stomata compared with needles from plantlets exposed to fluorescent light.

show abstract

Light Quality Affects Protocorm-Like Body (PLB) Formation, Growth and Development of In Vitro Plantlets of Phalaenopsis pulcherrima

Cited by 4 publications

References 30 publications

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Protocorm-Like Body Induction and Development Using In Vitro Culture Techniques and Biotechnological Methods

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Contact Info

Product

Resources

About