Light quality and natural ventilation have different effects on protocorm development and plantlet growth stages of the in vitro propagation of Epidendrum fulgens (Orchidaceae)

Fritsche, Yohan; Pinheiro, Marcos Vinícius Marques; Guerra, Miguel Pedro

doi:10.1016/j.sajb.2022.02.019

Cited by 10 publications

(5 citation statements)

References 66 publications

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“…Silva et al [ 63 ], in Pfaffia glomerata in vitro plantlets, increased in the size of vascular bundles and vessel elements under combinations of red and blue LED. In the same way, the central vascular bundle of plants from Epidendrum fulgens grown with natural ventilation under combinations of white/high blue, deep red/white/medium blue, and deep red/white/far red/medium blue LED had a higher content of sclerenchyma [ 64 ]. Furthermore, Gnasekaran et al [ 65 ] found that LED spectral quality alters plant chloroplast ultrastructure through the effects on starch accumulation in Z. officinale .…”

Section: Discussionmentioning

confidence: 99%

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Rojas-Vargas

Castander-Olarieta²,

Nascimento³

et al. 2023

Plants

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Pinus. ponderosa (P. Lawson and C. Lawson) is a commercial tree and one of the most important forest species in North America. Ponderosa pine suffers hardship when going through vegetative propagation and, in some cases, 15–30 years are needed to achieve full reproductive capacity. Based on previous works on P. ponderosa regeneration through in vitro organogenesis and trying to improve the published protocols, our objective was to analyze the influence of different types of explants, basal culture media, cytokinins, auxins, and light treatments on the success of shoot multiplication and rooting phases. Whole zygotic embryos and 44 µΜ 6-benzyladenine showed the best results in terms of explants survival. For shoot organogenesis, whole zygotic embryos and half LP (LP medium, Quoirin and Lepoivre, 1977, modified by Aitken-Christie et al., 1988) macronutrients were selected. A significant positive interaction between whole zygotic embryos and half LP macronutrients was found for the percentage of explants forming shoots. Regarding the light treatments applied, a significantly higher percentage of shoots elongated enough to be rooted was detected in shoots growing under blue LED at a light intensity of 61.09 µmol m−2 s−1. However, the acclimatization percentage was higher in shoots previously cultivated under fluorescent light at a light intensity of 61.71 µmol m−2 s−1. Anatomical studies using light microscopy and scanning electron microscopy showed the light treatments promoted differences in anatomical aspects in in vitro shoots; needles of plantlets exposed to red and blue LEDs revealed less stomata compared with needles from plantlets exposed to fluorescent light.

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Section: Discussionmentioning

confidence: 99%

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Rojas-Vargas

Castander-Olarieta²,

Nascimento³

et al. 2023

Plants

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“…The number of leaves, shoots, roots, and fresh weight was greater for plants developed in TIBS than those generated in the NV system. There was a 2-fold increase in root number for plants grown in TIBS compared to those in NV system, even though there was a significant impact on stomata number and photosynthetic pigment contents [33] Mokara Leuen Berger Gold Callus tissue SSS and TIS MS incorporated with BA (0.5 mg L −1 ), B1 (5 mg L −1 ), adenin sulfate (10 mg L −1 ), peptone (1 g L −1 ), CW (10%) and sucrose (20 g L −1 ) for SSS; MS supplemented with CW (10%), sucrose (20 g L −1 ) and other growth regulators at different concentrations for TIS.…”

Section: Min Immersion Every H Durationmentioning

confidence: 90%

“…Application of a temporary immersion system (TIS) can significantly reduce production costs, making it economically viable by allowing scaleup and full automation and eliminating inherent plant physiological problems associated with SS and continuous liquid culture systems [28,29]. TIS has been employed to propagate orchid species using different media, growth regulators, and explants [30][31][32][33]. The present review paper emphasizes different aspects of in vitro orchid propagation, conventional SS and TIS, benefits and weaknesses, and their applications for efficient large-scale plant production.…”

Section: Introductionmentioning

confidence: 99%

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

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Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation.

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“…At this level of ventilation openings, operating energy considers the major role of windows as the link between the external and internal environments, with great importance for energy performance and thermal comfort [9]. Ventilation openings provide natural light for the interior environment and are responsible for natural ventilation and passive cooling [10]. Effective assessment and design of the ventilation openings in construction projects could lessen the necessity of using the heating, ventilation, and air conditioning (HVAC) systems and, consequently, reduce the energy consumption in buildings [11].…”

Section: Introductionmentioning

confidence: 99%

Influence of Ventilation Openings on the Energy Efficiency of Metal Frame Modular Constructions in Brazil Using BIM

Najjar,

De Araujo,

Oladimeji

et al. 2023

Eng

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Construction projects demand a higher amount of energy predominantly for heating, ventilation, and illumination purposes. Modular construction has come into the limelight in recent years as a construction method that uses sustainable building materials and optimizes energy efficiency. Ventilation openings in buildings are designed to facilitate air circulation by naturally driven ventilation and could aid in reducing energy consumption in construction projects. However, a knowledge gap makes it difficult to propose the best dimensions of ventilation openings in buildings. Hence, the aim of this work is to empower the decision-making process in terms of proposing the best ventilation opening dimensions toward sustainable energy use and management in buildings. A novel framework is presented herein to evaluate the impact and propose the best dimensions of ventilation openings for metal frame modular construction in Brazil, using building information modeling. The ventilation openings were constructed and their dimensions evaluated in eight Brazilian cities, based on the bioclimatic zone (BioZ) classification indicated in ABNT NBR 15220: Curitiba (1st BioZ); Rio Negro (2nd BioZ); São Paulo (3rd BioZ); Brasília (4th BioZ); Campos (5th BioZ); Paranaíbe (6th BioZ); Goiás (7th BioZ); and Rio de Janeiro (8th BioZ). The study results show that the energy consumption of the same building model would vary based on the dimensions of ventilation openings for each BioZ in Brazil. For instance, modeling the same modular construction unit in the city of Rio Negro could consume around 50% of the energy compared to the same unit constructed in the city of Rio de Janeiro, using the small opening sizes based on the smallest dimensions of the ventilation openings. Similarly, modeling the construction unit in Curitiba, São Paulo, Brasília, Campos, Paranaíba, and Goiás could reduce energy consumption by around 40%, 34%, 36%, 18%, 20%, and 16%, respectively, compared to constructing the same building in the city of Rio de Janeiro, using the small opening sizes based on the smallest dimensions of the ventilation openings. This work could help practitioners and professionals in modular construction projects to design the best dimensions of the ventilation openings based on each BioZ towards increasing energy efficiency and sustainability.

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Light quality and natural ventilation have different effects on protocorm development and plantlet growth stages of the in vitro propagation of Epidendrum fulgens (Orchidaceae)

Cited by 10 publications

References 66 publications

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Testing Explant Sources, Culture Media, and Light Conditions for the Improvement of Organogenesis in Pinus ponderosa (P. Lawson and C. Lawson)

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Influence of Ventilation Openings on the Energy Efficiency of Metal Frame Modular Constructions in Brazil Using BIM

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