Light-regulated host–guest interaction as a new strategy for intracellular PEG-detachable polyplexes to facilitate nuclear entry

Abstract

PEG-detachable polyplexes were constructed for the first time via host-guest interactions between β-cyclodextrin and azobenzene. The polyplexes had excellent colloidal stability and competition stability. Moreover, the intracellular light-regulated dePEGylation facilitated DNA release and nuclear entry, thus resulting in efficient transfection.

Nonlinear Supramolecular Polymers for Therapeutic Applications

“…By modifying a β‐CD core with several temperature‐responsive poly ( N ‐isopropylacrylamide) and biocompatible poly(oligo(ethylene glycol)acrylate) block copolymer arms, the resultant star structure is capable to form stable nanoparticles at body temperature, enhancing the cellular uptake and overcoming drug resistance from membrane pump mediated drug efflux. Similarly, a few nonlinear topological drug carries based on CD and guest molecules with responsibility were designed by taking advantage of nonlinear structure and interactions …”

Development of Supramolecular Pseudo‐Block Conjugates Based on Star‐Shaped Polycation for DNA Delivery

“…On the other hand, PEI/deoxyribonucleic acid (DNA) polyplexes tend to rapidly aggregate in physiological salt condition . PEGylation is a common approach to improve the colloidal stability and prolong the blood circulation time in vivo . Host–guest interaction provides a facile strategy to construct PEGylated polyplexes due to easy preparation process .…”

“…PEGylation is a common approach to improve the colloidal stability and prolong the blood circulation time in vivo . Host–guest interaction provides a facile strategy to construct PEGylated polyplexes due to easy preparation process . Recently, we developed poly(ethylene glycol) (PEG)‐detachable polyplexes and multifunctional nanoparticles via the light‐regulated host–guest interaction between CD and azobenzene (Az) .…”

“…Host–guest interaction provides a facile strategy to construct PEGylated polyplexes due to easy preparation process . Recently, we developed poly(ethylene glycol) (PEG)‐detachable polyplexes and multifunctional nanoparticles via the light‐regulated host–guest interaction between CD and azobenzene (Az) . In this work, supramolecular pseudo‐block conjugates based on star‐shaped polycation were developed as novel nonviral gene vector (Figure ).…”

Self‐Assembly of Near‐Monodisperse Redox‐Sensitive Micelles from Cholesterol‐Conjugated Biomimetic Copolymers

“…Because they can largely meet the demands of an ideal delivery system by providing high stability during in vivo circulation while providing rapid drug release after localizing at the targeted cells. A variety of shedding mechanisms have been reported, such as light sensitivity, pH sensitivity, reduction sensitivity, and enzyme digestion . In particular, reduction‐sensitive shedding micelles are paid great attention due to the high discrepancy of the glutathione (GSH) level between intracellular (1–10 mM) and extracellular milieu (≈2 μM) .…”