Light-responsive gene expression in cyanobacteria

Golden, Susan S.

doi:10.1128/jb.177.7.1651-1654.1995

Cited by 123 publications

(62 citation statements)

References 62 publications

(57 reference statements)

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“…The Synechococcus sp. PCC 7942 psbA gene family is posttranscriptionally regulated by light (Golden, 1995). The psbAI and psbAIII transcripts are degraded faster under high-light conditions, and this regulation requires de novo transcription and translation after exposure to high light.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional and Posttranscriptional Control of mRNA fromlrtA, a Light-Repressed Transcript inSynechococcus sp. PCC 70021

Samartzidou

Widger

1998

Plant Physiology

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Transcription regulation and transcript stability of a lightrepressed transcript, lrtA, from the cyanobacterium Synechococcus sp. PCC 7002 were studied using ribonuclease protection assays. The transcript for lrtA was not detected in continuously illuminated cells, yet transcript levels increased when cells were placed in the dark. A lag of 20 to 30 min was seen in the accumulation of this transcript after the cells were placed in the dark. Transcript synthesis continued in the dark for 3 h and the transcript levels remained elevated for at least 7 h. The addition of 10 M rifampicin to illuminated cells before dark adaptation inhibited the transcription of lrtA in the dark. Upon the addition of rifampicin to 3-h dark-adapted cells, lrtA transcript levels remained constant for 30 min and persisted for 3 h. A 3-h half-life was estimated in the dark, whereas a 4-min half-life was observed in the light. Extensive secondary structure was predicted for this transcript within the 5 untranslated region, which is also present in the 5 untranslated region of lrtA from a different cyanobacterium, Synechocystis sp. PCC 6803. Evidence suggests that lrtA transcript stability is not the result of differences in ribonuclease activity from dark to light. Small amounts of lrtA transcript were detected in illuminated cells upon the addition of 25 g mL ؊1 chloramphenicol. The addition of chloramphenicol to dark-adapted cells before illumination allowed detection of the lrtA transcript for longer times in the light relative to controls without chloramphenicol. These results suggest that lrtA mRNA processing in the light is different from that in the dark and that protein synthesis is required for light repression of the lrtA transcript.

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Section: Discussionmentioning

confidence: 99%

Transcriptional and Posttranscriptional Control of mRNA fromlrtA, a Light-Repressed Transcript inSynechococcus sp. PCC 70021

Samartzidou

Widger

1998

Plant Physiology

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“…Several similarities support this assumption. As in chloroplasts, the transcription of many cyanobacterial photosynthesis genes is influenced by light and photosynthetic electron transport [37][38][39]. Under photoautotrophic conditions, DCMU and DBMIB can be used to manipulate the redox state of the PQ pool in the same way as in chloroplasts [40,41].…”

Section: Redox Signalling Pathways In Cyanobacteria -A Model For Chlomentioning

confidence: 99%

Chloroplast redox signals: how photosynthesis controls its own genes

Pfannschmidt

2003

Trends in Plant Science

448

301

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“…Post-transcriptional regulation is an important layer of control in the response of a family of cyanobacterial photosystem II genes to changes in light intensity (Kulkarni et al, 1992;Golden, 1995). The three psbA genes of the cyanobacterium Synechococcus sp.…”

Section: Introductionmentioning

confidence: 99%

mRNA stability is regulated by a coding‐region element and the unique 5′ untranslated leader sequences of the three Synechococcus psbA transcripts

Kulkarni

Golden

1997

Molecular Microbiology

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SummaryThe psbAI and psbAIII transcripts in Synechococcus sp. strain PCC 7942 are subject to accelerated turnover when cells are exposed to high light intensities, but psbAII message stability is unaffected. We used a psbAI 'minigene' which has a part of the coding sequence removed as a reporter gene in order to identify the cis-acting elements of the transcript that determine stability. While engineering the minigene to optimally mimic the native gene, we identified a stabilizer element within the open reading frame, corresponding to the coding region for the first membrane span of the D1 protein, the presence of and translation through which was essential for normal psbA mRNA stability. We propose that this stabilizer is a site for ribosome pausing, and that accumulation of ribosomes on the transcript upstream of the pause site increases stability. To identify the elements that regulate the differential responses of the psbA transcripts to high-light growth, sequences from psbAII and psbAIII were substituted in the psbAI minigene reporter. The chimeric reporter transcripts established that the psbAI and psbAIII untranslated leaders determine the faster turnover of these messages. The untranslated leader regions of the psbA transcripts may regulate mRNA stability by modulating translation and thereby stability, or by recruiting RNA-binding proteins that affect mRNA turnover more directly.

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Light-responsive gene expression in cyanobacteria

Cited by 123 publications

References 62 publications

Transcriptional and Posttranscriptional Control of mRNA fromlrtA, a Light-Repressed Transcript inSynechococcus sp. PCC 70021

Transcriptional and Posttranscriptional Control of mRNA fromlrtA, a Light-Repressed Transcript inSynechococcus sp. PCC 70021

Chloroplast redox signals: how photosynthesis controls its own genes

mRNA stability is regulated by a coding‐region element and the unique 5′ untranslated leader sequences of the three Synechococcus psbA transcripts

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