Light sheet Raman micro-spectroscopy

Müller, Walter; Kielhorn, Martin; Schmitt, Michael; Popp, Jürgen; Heintzmann, Rainer

doi:10.1364/optica.3.000452

Cited by 54 publications

(49 citation statements)

References 24 publications

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“…In addition, LSFM has been combined with SR techniques such as STORM [48,[80][81][82], STED [92], and structured illumination [69,75,93]. The versatility of LSFM also allows combining it with more established techniques, such as FLIM [94][95][96], FRET [96], hyperspectral fluorescence [97], Raman [98][99][100][101], and OPT [102,103]. The combined advantages of LSFM are thus shared with multimodal approaches and allow extracting structural, functional, chemical, and molecular information from the sample, enabling a comprehensive interpretation of the biological process of study.…”

Section: 1g Lsfm Allows Multimodal Imaging Of Biological Samplesmentioning

confidence: 99%

“…Unfortunately, producing a Raman image can be a slow process due to the long integration times needed to detect the weak Raman signal. In this sense, highly optically efficient LSFM is an amenable platform for Raman spectroscopy [99][100][101]116]. For more detail on Raman LS implementations, please see Section 6.1c.…”

Section: Elastic and Inelastic Scatteringmentioning

confidence: 99%

“…Recently, another LSFM Raman implementation combined with a novel Fourier transform (FT)-based spectral imaging method was presented [101]. In this technique, the full 2D image obtained with the LSFM is split into the two arms of a Michelson interferometer and recombined on the CCD camera; see Fig.…”

Section: 1c Hyperspectral Detection (Raman)mentioning

confidence: 99%

“…The spectra are recovered using a Fourier-transform imaging spectrometer (red path). See the main text for a detailed description [101].…”

Section: 1d Non-filtered Detection (Elastic Scattering)mentioning

confidence: 99%

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Light-sheet microscopy: a tutorial

et al. 2018

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This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted.

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Section: 1g Lsfm Allows Multimodal Imaging Of Biological Samplesmentioning

confidence: 99%

Section: Elastic and Inelastic Scatteringmentioning

confidence: 99%

Section: 1c Hyperspectral Detection (Raman)mentioning

confidence: 99%

“…The spectra are recovered using a Fourier-transform imaging spectrometer (red path). See the main text for a detailed description [101].…”

Section: 1d Non-filtered Detection (Elastic Scattering)mentioning

confidence: 99%

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Light-sheet microscopy: a tutorial

et al. 2018

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“…However, Michelson designs are not common-path and typically phase-unstable due to their sensitivity to external vibrations. Moreover, in the original form, their pitch and yaw stability are hard to maintain during interferometer mirror translation [13]. Attempts have, however, been made to improve Michelson designs.…”

Section: Introductionmentioning

confidence: 99%

Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy

Wadduwage

Singh

Choi

et al. 2017

Optica

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Imaging Fourier-transform spectroscopy (IFTS) is a powerful method for biological hyperspectral analysis based on various imaging modalities, such as fluorescence or Raman. Since the measurements are taken in the Fourier space of the spectrum, it can also take advantage of compressed sensing strategies. IFTS has been readily implemented in high-throughput, high-content microscope systems based on wide-field imaging modalities. However, there are limitations in existing wide-field IFTS designs. Non-common-path approaches are less phase-stable. Alternatively, designs based on the common-path Sagnac interferometer are stable, but incompatible with high-throughput imaging. They require exhaustive sequential scanning over large interferometric path delays, making compressive strategic data acquisition impossible. In this paper, we present a novel phase-stable, near-common-path interferometer enabling high-throughput hyperspectral imaging based on strategic data acquisition. Our results suggest that this approach can improve throughput over those of many other wide-field spectral techniques by more than an order of magnitude without compromising phase stability.

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Multi‐Scale Imaging

2017

Super‐Resolution Microscopy

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Light sheet Raman micro-spectroscopy

Cited by 54 publications

References 24 publications

Light-sheet microscopy: a tutorial

Light-sheet microscopy: a tutorial

Near-common-path interferometer for imaging Fourier-transform spectroscopy in wide-field microscopy

Multi‐Scale Imaging

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