Light spectral composition affects metabolic response and flowering in non-vernalized Ranunculus asiaticus L.

Carillo, Petronia; Modarelli, Giuseppe Carlo; Fusco, Giovanna Marta; Dell’Aversana, Emilia; Arena, Carmen; Pascale, Stefania De; Paradiso, Roberta

doi:10.1016/j.envexpbot.2021.104649

Cited by 6 publications

(7 citation statements)

References 51 publications

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“…In the environmental and cultural conditions of our experiment, in Control plants from only rehydrated tuberous roots, flowering earliness did not change significantly between the hybrids, as observed in a previous experiment on plants of the same genotypes obtained with the same preparation procedure [ 13 ]. Cold treatments of rehydrated tuberous roots, similar to our V2 procedure, have been proved to anticipate flowering in different Ranunculus hybrids [ 5 ]; however, in our experiment the exposure to 3.5 °C for 2 weeks, which is the most common treatment in breeding farms, was effective in the hybrid MDR but ineffective or detrimental in MBO one.…”

Section: Discussionsupporting

confidence: 70%

“…Cultivation of Ranunculus asiaticus L. has been rising during the last years all over the world, also thanks to the breeding and the development of new hybrids, and vernalization of propagation material is a common practice for the production scheduling of cut flowers, as in many ornamental geophytes [ 1 ]. Despite this, little is known about the plant physiology of this species and, apart from some our recent studies [ 11 , 12 , 13 , 14 ], no information seems to be available on the primary and secondary metabolism as affected by cold treatments of propagation material. We investigated the influence of two hybrids of R. asiaticus L., characterized by different flowering earliness, and three preparation procedures of tuberous roots, including only rehydration and two times of exposure to cold temperature after rehydration, on photosynthesis, metabolic profile and flowering of plants grown in pot in cold glasshouse.…”

Section: Introductionmentioning

confidence: 99%

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Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of Ranunculus asiaticus L.

Fusco

Carillo

Nicastro

et al. 2023

Plants

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In Ranunculus asiaticus L., vernalization of propagation material is a common practice for the production scheduling of cut flowers, however little is known about the plant physiology and metabolism of this species as affected by cold treatments. We investigated the influence of two hybrids, MBO and MDR, and three preparation procedures of tuberous roots, only rehydration (control, C), and rehydration plus vernalization at 3.5 °C for 2 weeks (V2) and for 4 weeks (V4), on plant growth and flowering, leaf photosynthesis, and leaf metabolic profile in plants grown in pot in a cold greenhouse. Net photosynthesis (NP) was higher in MDR than in MBO. In the two genotypes, the NP did not change in V2 and increased in V4 compared to C in MBO, while was unaffected by vernalization in MDR. Quantum yield of PSII electron transport (ΦPSII), linear electron transport rate (ETR) and non-photochemical quenching (NPQ) did not differ in the two hybrids, whereas maximal PSII photochemical efficiency (Fv/Fm) was higher in MBO than in MDR. Fluorescence indexes were unaffected by the preparation procedure, except for ETR, which decreased in V2 compared to C and V4 in MDR. A significant interaction between genotype and preparation procedure was found in plant leaf area, which was reduced only in V4 in MBO, while decreased in both the vernalization procedures in MDR. In Control plants, flowering started in 65 days in MBO and 69 days in MDR. Compared to controls, both the vernalization treatments anticipated flowering in MDR, while they were detrimental or only slightly efficient in promoting flowering in MBO. Vernalization always reduced the quality of flower stems in both the hybrids.

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Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of Ranunculus asiaticus L.

Fusco

Carillo

Nicastro

et al. 2023

Plants

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“…In this experiment, plants of the hybrids MBO (early flowering) and MDR (medium earliness) of Ranunculus asiaticus L. were subjected to three preparation procedures of tuberous roots and grown in an unheated glasshouse in the south of Italy from September to March. In our environmental and cultural conditions, flowering of control plants from only rehydrated roots started at the beginning of January, and the time for flowering was similar in the hybrids, despite the different earliness expected, and flower stem characteristics were comparable, as previously observed on the same genotypes subjected to the same preparation procedure [23].…”

Section: Discussionsupporting

confidence: 78%

“…We carried out a series of experiments aiming at elucidating the influence of temperature [ 17 , 18 ] and photoperiod [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ] and their interaction on plant physiology and metabolism and the flowering process of R. asiaticus . In the present experiment, we investigated the influence of three preparation procedures of tuberous roots—only rehydration and two times of exposure to cold temperatures after rehydration—on plant growth and metabolic profiles in two hybrids with different flowering earliness grown in pots in an unheated glasshouse.…”

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling in Tuberous Roots of Ranunculus asiaticus L. as Influenced by Vernalization Procedure

Fusco,

Carillo,

Nicastro

et al. 2023

Plants

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Ranunculus asiaticus L. is an ornamental geophyte. In commercial practice, it is mainly propagated by rehydrated tuberous roots. Vernalization before planting is a common practice to overcome the natural dormancy of tuberous roots; however, little is known about the mechanisms underlying the plant’s response to low temperatures. We investigated the influence of three preparation procedures of tuberous roots, only rehydration (control, C), and rehydration plus vernalization at 3.5 °C for 2 weeks (V2) and for 4 weeks (V4), on plant growth, leaf photosynthesis, flowering, and metabolism in plants of two hybrids, MBO (early flowering, pale orange flower) and MDR (medium earliness, bright orange flower), grown in pots in an unheated greenhouse. We reported the responses observed in the aerial part in a previous article in this journal. In this paper, we show changes in the underground organs in carbohydrate, amino acids, polyphenols, and protein levels throughout the growing cycle in the different plant stages: pre-planting, vegetative growth, and flowering. The metabolic profile revealed that the two hybrids had different responses to the root preparation procedure. In particular, MBO synthesized GABA and alanine after 2 weeks and sucrose after 4 weeks of vernalization. In contrast, MDR was more sensitive to vernalization; in fact, a higher synthesis of polyphenols was observed. However, both hybrids synthesized metabolites that could withstand exposure to low temperatures.

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“…Its abundant distribution stretches through the Mediterranean basin and Asia. It is an annually cultivated cut flower crop (Carillo et al, 2021). Ranunculus flowers are well-known for their bright colors and vibrant patterns.…”

Section: Introductionmentioning

confidence: 99%

Genetic Analysis and Morphological Profiling of Ranunculus Genotypes

BARAN¹,

KHAN²,

HABIB³

et al. 2023

SABRAOJBG

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Ranunculus is a well-known flower having a great economic value for its bright colors and vibrant patterns. Given the importance of the ranunculus cut flower, conducting this study elucidated the genetic behavior and variability of various morphological attributes in ranunculus genotypes. Assessment of six ranunculus genotypes measured germination percentage (%), days to emergence, days to maturity, stem length, stem thickness, flowers per plant, flower size, and flower duration on the plant. Analysis of variance depicted significant variation in all the studied traits except days to emergence, number of stems, and germination percentage. Clustering ranunculus genotype with the Euclidean distances-based hierarchical clustering resulted in two clusters of the genotype. One genotype (red) did not join any group, indicating an early separation of this genotype during the evolution of Ranunculus species. Correlation analysis showed that most studied traits negatively correlated, with only a few positively correlating traits. The number of flowers per plant and stem length showed a d positive correlation (0.7437 and 0.8064, respectively). Overall, the results showed that the red, yellow, and rose genotypes are the best performers for cultivation to produce better-quality flowers. Genetic analysis using line × tester analysis revealed higher values for the SCA component than GCA, suggesting non-additive gene action for most traits under study. Moreover, the hybrids developed in the current study developed new color combinations/shades. These hybrids could further benefit ranunculus stable variant improvement.

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Light spectral composition affects metabolic response and flowering in non-vernalized Ranunculus asiaticus L.

Cited by 6 publications

References 51 publications

Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of Ranunculus asiaticus L.

Vernalization Procedure of Tuberous Roots Affects Growth, Photosynthesis and Metabolic Profile of Ranunculus asiaticus L.

Metabolic Profiling in Tuberous Roots of Ranunculus asiaticus L. as Influenced by Vernalization Procedure

Genetic Analysis and Morphological Profiling of Ranunculus Genotypes

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