Light Supports Neurite Outgrowth of Human Neural Progenitor Cells<i>In Vitro</i>: The Role of P2Y Receptors

Anders, Juanita J.; Romanczyk, Tara B.; Ilev, Ilko K.; Moges, Helina; Longo, Leonardo; Wu, Xingjia; Waynant, Ronald W.

doi:10.1109/jstqe.2008.916181

Cited by 34 publications

(23 citation statements)

References 33 publications

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“…Differentiation of neural progenitor cells is also promoted by NIR. The radiation induces the production of ATP, which subsequently activates P2Y receptors for neurite outgrowth (19). Although the molecular mechanisms by which low-energy radiation affects various cellular activities are unclear, accumulating evidence indicates that NIR may be used in multiple clinical treatments (6)(7)(8)(9)(10).…”

Section: Discussionmentioning

confidence: 99%

Treatment with near-infrared radiation promotes apoptosis in pancreatic cancer cells

et al. 2015

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Abstract. Cancer remains one of the leading causes of human mortality worldwide. Radiation and chemotherapy are commonly used for cancer treatment; however, the combination of these therapies and surgery do not completely eradicate cancer cells. Near-infrared radiation (NIR) is a low-energy form of radiation that exerts multiple effects on mammalian cells. Previous studies have reported that NIR induces DNA double-strand breaks and apoptosis of cancer cells. In the present study, a 915-nm laser was used to examine the effects of NIR on pancreatic cancer cells. Irradiation of pancreatic cancer cells using a 915-nm laser significantly induced caspase-3 activation and apoptosis. In addition, the combination of gemcitabine treatment and a 915-nm laser synergistically increased the number of apoptotic cells. The results of the present study indicate the use of infrared irradiation and chemotherapy may be a possible therapy for the treatment of cancer. IntroductionAlthough considerable knowledge of cancer biology has been acquired over the last few decades, cancer remains one of the major causes of mortality worldwide. Surgical resection is the most effective treatment; however, in the majority of cases, the tumor cells have already advanced locally or metastasized to distant organs by the time of diagnosis. Chemotherapy, a commonly used treatment for cancers that may not be removed by surgery, often results in severe side-effects due to the delivery of drugs to normal cells (1). As a result of these side-effects, the high doses of chemotherapy required to completely eradicate cancer cells are not tolerated in humans. In addition, the majority of cancer cells eventually acquire drug resistance and result in mortality. Therefore, the development of additional drugs or novel methods for the treatment of cancer is urgently required.Radiation therapy is a highly targeted and effective way to treat certain types of cancer with limited metastasis (2). This treatment uses high-energy radiation, such as X-rays, γ-rays and charged particles. High-energy radiation induces double strand breaks in DNA, which promotes apoptosis of cancer cells (3). However, the radiation also results in severe damage to normal cells. Therefore, irradiation must be limited to the cancerous area to minimize the side-effects of this treatment. In contrast to these high-energy radiations, less energetic forms of radiation have not yet been fully evaluated for their anti-cancer functions. Near-infrared radiation (NIR) is a shorter wavelength of radiation in the infrared region of the spectrum, ranging between 750 and 2,500 nm. It has previously been reported that irradiation in the near-infrared region has multiple effects on cells. NIR induces the proliferation of keratinocytes (4), promotes cell attachment (5), attenuates the infarct size following myocardial infarction in rats and dogs (6,7) and regenerates and induces the proliferation of skeletal muscle (8). In addition, NIR has been demonstrated to have an inhibitory effect on advanced neoplasia ...

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Section: Discussionmentioning

confidence: 99%

Treatment with near-infrared radiation promotes apoptosis in pancreatic cancer cells

et al. 2015

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“…Несмотря на то, что существует ряд работ, ис-следующих роль длины волны [16,27,55,67,93,95], складывается впечатление, что хотя, вероятно, и су-ществует ее оптимальное значение в каждых опре-деленных условиях, данный параметр не является основным. В конечном счете, хороших результатов можно добиться практически при любой длине вол-ны.…”

Section: Discussionunclassified

The application of stem cells, visible and infrared light in regenerative medicine. Part 1

Emel'yanov

Kiryanova

2015

Vopr. kurortol. fizioter. lech. fiz. kul't.

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51Основной задачей регенеративной медицины (РМ) является восстановление утраченных в резуль-тате тех или иных болезней или повреждений струк-туры и функции органов и тканей. человеческий организм может регенерировать на уровне клеточ-ного пласта, состоящего из одного или, может быть, нескольких видов близкородственных клеток. Дан-ное свойство используется в медицине для восста-новления кожного эпителия, при переломах костей, разрывах сухожилий, мышц, повреждении нервных стволов и пр. Однако после повреждения несколь-ких слоев тканей (например, кожного эпителия и подлежащей дермы) или нарушения структуры тка-ней и органов всегда остаются рубцы, что является наглядным свидетельством неполной регенерации. при подобных повреждениях для полного восста- Health, ul. kirochnaya, 41, Sankt-Peterburg, Russia, 191015 Несмотря на длительную историю фотохромотерапии, до сих пор не существует достаточной теоретической базы для вы-бора таких параметров облучения, как плотность мощности, доза облучения и время экспозиции. Также не существует обобщений применения света в области регенеративной медицины. Цель обзора -обобщить данные экспериментальных исследований, связанных с воздействием света видимого и инфракрасного диапазонов на стволовые клетки (Ск) и как следствие уточнить рекомендации по выбору параметров облучения и углубить их научное обоснование. Результаты. Проанализированы 78 оригинальных статей, в которых представлены результаты исследований, выполненных на различ-ных типах Ск. их качество, количество и структура свидетельствуют о начальном (инициальном) этапе развития данной области медицины. Анализ выявил значимость типа Ск при выборе параметров облучения. Наибольших прибавок скоро-стей пролиферации и дифференцировки авторы добивались при высоких плотностях мощности (более 50 мвт/см 2 ) и низ-ких дозах (около 1 Дж/см 2 ) и времени экспозиции (секунды). Заключение. общие выводы для 1-й и 2-й частей данного обзора будут представлены в следующем номере данного журнала. Ключевые слова: фотохромотерапия, свет, стволовые клетки, фототерапия, регенеративная медицина.Objective: the present article was designed to overview the experimental studies of visible and infrared light irradiation of human and animal stem cells (SC) in vitro and in vivo for the evaluation of its photobiomodulatory effects. The results will be used to elaborate substantiation for the choice of the parameters of SC light irradiation and to develop recommendations for the application of this method in regenerative medicine (Rm). Background: the clinical application of light irradiation is a matter of contrsy, in the first place due to the difficulties encountered in the rational choice of irradiation parameters. The theoretical substantiation of such choice remains a stumbling block too despite the long history of photoghromotherapy. There is thus far no reliable theoretical basis for the adequate choice of such irradiation parameters as power density, radiation dose, and exposure time. The experiences with the light application for the purpose of ...

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“…It was recently demonstrated that not only do ADSCs respond well to external stimuli, including LILI, as a mechanism of inducing increased viability and proliferation, but differentiation into other cell lineages can be achieved with minimal invasive techniques such as careful selection and addition of growth factor combinations. Even in the absence of growth factors, the use of laser irradiation to stimulate differentiation has been shown in Normal Human Neural Progenitor Cells (NHNPCs) in vitro where NHNPCs are not only capable of being sustained by light in the absence of growth factors, but they are also able to differentiate normally as assessed by neurite formation [16]. Our work has focused on the ability of laser irradiation to increase proliferation of ADSCs, maintain their SC character and increase the rate and maintenance of differentiation of ADSCs into smooth muscle cells [17].…”

Section: Low Intensity Laser Irradiation (Lili)mentioning

confidence: 99%

Inducing stem cell differentiation using low intensity laser irradiation: a possible novel therapeutic intervention

Abrahamse

2011

Open Life Sciences

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Developments in adult stem cell (ASC) potentiation have contributed to excitement in the field of stem cell-based therapy. The use of ASCs not only increases therapeutic treatment possibilities but successful use of multipotent cells for gene therapy has been demonstrated in animal models [1]. Concurrent ability of stem cells (SCs) to either contribute to disease development, as identified in cancer stem cells (CSCs), or to replace diseased tissue by induced differentiation using selected growth factors, has highlighted the intricate molecular and cellular mechanisms. Adipose derived stem cells (ADSCs) are capable of self-renewal and respond well to induced differentiation [2]. Auto-immunity and transplant rejection may become minor limitations when selective induction of immunological nonresponsiveness to specific antigens or tissues become possible using autologous cell sources [3]. CSCs initiate tumorogenesis, can generate differentiated daughter cells or undergo self-renewal while thought to instigate tumour regeneration post-treatment. Therapy targeting CSCs has failed to provide feasible alternatives to conventional cancer treatment. Low intensity laser irradiation (LILI), induce a biostimulatory response in several tissue types in addition to a dose-response effect to the detriment of cellular degeneration. Potential of LILI to induce CSC differentiation and subsequent cytotoxic therapy to prevent tumour regeneration is explored in this mini-review.

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Light Supports Neurite Outgrowth of Human Neural Progenitor CellsIn Vitro: The Role of P2Y Receptors

Cited by 34 publications

References 33 publications

Treatment with near-infrared radiation promotes apoptosis in pancreatic cancer cells

Treatment with near-infrared radiation promotes apoptosis in pancreatic cancer cells

The application of stem cells, visible and infrared light in regenerative medicine. Part 1

Inducing stem cell differentiation using low intensity laser irradiation: a possible novel therapeutic intervention

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