Lighting up single-mRNA translation dynamics in living cells

Cialek, Charlotte; Koch, Amanda; Galindo, Gabriel; Stasevich, Timothy J.

doi:10.1016/j.gde.2020.04.003

Cited by 15 publications

(12 citation statements)

References 63 publications

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“…Secondary, recruited integrins might add dynamism and competition within integrin clusters that allow cells to switch their adhesive behavior, and could be addressed by using single particle tracking to watch the interaction of two integrins within the same cells. The use of a growing range of small molecule tags ( 237 ), namely frankenbodies ( 238 ), SunTag ( 239 ), and MoonTag ( 240 ), and cell permeable bright stable organic dyes ( 241 ) may allow this type of nanoscale investigation to be undertaken.…”

Section: The Future Of Trnk Study: Linking Single Molecule and Clustementioning

confidence: 99%

Natural Killer Cell Integrins and Their Functions in Tissue Residency

Shannon

Mace

2021

Front. Immunol.

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Integrins are transmembrane receptors associated with adhesion and migration and are often highly differentially expressed receptors amongst natural killer cell subsets in microenvironments. Tissue resident natural killer cells are frequently defined by their differential integrin expression compared to other NK cell subsets, and integrins can further localize tissue resident NK cells to tissue microenvironments. As such, integrins play important roles in both the phenotypic and functional identity of NK cell subsets. Here we review the expression of integrin subtypes on NK cells and NK cell subsets with the goal of better understanding how integrin selection can dictate tissue residency and mediate function from the nanoscale to the tissue environment.

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Section: The Future Of Trnk Study: Linking Single Molecule and Clustementioning

confidence: 99%

Natural Killer Cell Integrins and Their Functions in Tissue Residency

Shannon

Mace

2021

Front. Immunol.

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“…In these systems, one type of nanobody fused with a fluorescent protein is used to bind special tandem short peptides (typically 24 copies) fused with a target protein; thus, one protein will be labeled by many fluorescent proteins from antibody–antigen recognition. Using this system together with RNA-protein recognition systems such as the MS2 system [ 38 ] and PP7 system [ 39 ], researchers studied the transcription and translation processes at the single-molecule level [ 40 , 41 , 42 ]. Although these amplification systems have been used effectively in plants [ 43 ], they may not be suitable for single-molecule tracking, because three tandem fluorescent proteins will affect the movement of protein [ 44 ].…”

Section: Single-molecule Labeling and Imaging Strategiesmentioning

confidence: 99%

Single-Molecule Imaging in Living Plant Cells: A Methodological Review

Guo

Zhang

Wang

et al. 2021

IJMS

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Single-molecule imaging is emerging as a revolutionary approach to studying fundamental questions in plants. However, compared with its use in animals, the application of single-molecule imaging in plants is still underexplored. Here, we review the applications, advantages, and challenges of single-molecule fluorescence imaging in plant systems from the perspective of methodology. Firstly, we provide a general overview of single-molecule imaging methods and their principles. Next, we summarize the unprecedented quantitative details that can be obtained using single-molecule techniques compared to bulk assays. Finally, we discuss the main problems encountered at this stage and provide possible solutions.

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“…Does local, co-translational assembly of protein complexes occur in synapses (Schwarz and Beck, 2019), and if so, does neural activity play a role? New techniques, including site-directed control of protein synthesis (Kim et al, 2020), live imaging of mRNA translation dynamics (Cialek et al, 2020), and advanced ribosome profiling applications (Shiber et al, 2018;Ingolia et al, 2019) will put these and other questions within experimental reach in the coming years.…”

Section: Local Protein Translation Underlies Activity-dependent Plastmentioning

confidence: 99%

A Picture Worth a Thousand Molecules—Integrative Technologies for Mapping Subcellular Molecular Organization and Plasticity in Developing Circuits

Minehart

Speer

2021

Front. Synaptic Neurosci.

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A key challenge in developmental neuroscience is identifying the local regulatory mechanisms that control neurite and synaptic refinement over large brain volumes. Innovative molecular techniques and high-resolution imaging tools are beginning to reshape our view of how local protein translation in subcellular compartments drives axonal, dendritic, and synaptic development and plasticity. Here we review recent progress in three areas of neurite and synaptic study in situ—compartment-specific transcriptomics/translatomics, targeted proteomics, and super-resolution imaging analysis of synaptic organization and development. We discuss synergies between sequencing and imaging techniques for the discovery and validation of local molecular signaling mechanisms regulating synaptic development, plasticity, and maintenance in circuits.

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Lighting up single-mRNA translation dynamics in living cells

Cited by 15 publications

References 63 publications

Natural Killer Cell Integrins and Their Functions in Tissue Residency

Natural Killer Cell Integrins and Their Functions in Tissue Residency

Single-Molecule Imaging in Living Plant Cells: A Methodological Review

A Picture Worth a Thousand Molecules—Integrative Technologies for Mapping Subcellular Molecular Organization and Plasticity in Developing Circuits

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