Lignin nanoparticles are renewable and functional platforms for the concanavalin a oriented immobilization of glucose oxidase–peroxidase in cascade bio-sensing

Abstract

Lignin nanoparticles as functional renewable nanoplatform for the immobilization of cascade process in colorimetric biosensing of β-d-glucose.

Novel Nanoarchitectures Based on Lignin Nanoparticles for Electrochemical Eco-Friendly Biosensing Development

“…In particular, the three-layered Au(KNLPs/CoA/GOx) 3 biosensor showed an enhanced sensitivity of 15.85 µA/mM cm 2 , a linear range between 0.1 and 2.5 mM and a lower LOD of 0.02 mM, compared to the one-layered same platform. In addition, the increase in the current value clearly demonstrates the important role played by Con A, which allows a well-oriented immobilization of GOx and by LNPs due to their boosting effect in the activity of the overall electrocatalytic system, as previously hypothesized in our previous work [ 47 ].…”

“…This result, apparently in contrast with data previously reported, can be explained by taking into account the larger electrode coating of the KNLPs layer, which allows an immobilization of a much larger amount of enzyme. Moreover, the presence of Con A favors a correct orientation of the GOx enzyme during the immobilization process [ 47 ], enhancing the electrocatalytic performance of the system.…”

“…Lignin nanoparticles from organosolv lignin (OL) or kraft lignin (KL) were prepared according to nanoprecipitation procedures described in our previous works [ 47 ]. Briefly, a solution of OL or KL (2.0 mg in 1.2 mL of THF/H 2 O 5:1 v/v ) was added to Milli-Q water (in 3.8 mL) to obtain OLNPs and KLNPs.…”

“…In particular, LNPs have been used as renewable and sustainable platforms for the immobilization of redox enzymes, such as laccase [ 44 ], tyrosinase [ 45 ] and the enzymatic cascade of horseradish peroxidase (HRP) and glucose oxidase (GOx) [ 46 ]. The benign effect of the ordered immobilization of the two enzymes on the surface of LNPs has been reported, focusing on the role of concanavalin A (Con A), a lectin able to interact with the carbohydrate component of both glycoproteins HRP and GOx [ 46 ], in the increase in the activity of the system [ 47 , 48 ].…”

Access to tough and transparent nanocomposites via Pickering emulsion polymerization using biocatalytic hybrid lignin nanoparticles as functional surfactants

“…35,36,38,46,47 Additionally, LNPs as anionic polyelectrolytes adsorb cationic polymers and proteins, and a. Department of Materials and Environmental Chemistry, Stockholm University, 10691, Stockholm (Sweden) such coated particles are of interest for different applications ranging from catalytic transformations 48,49 to sensors 50,51 and water purification. 52 These non-covalently modified LNPs have also been demonstrated as stabilizers for durable Pickering emulsions 53,54 and vessels for the polymerization of hydrophobic monomers stabilized in water.…”