Limb Salvage for Osteosarcoma: Current Status with a Review of Literature

Agarwal, Manish; Nayak, Prakash

doi:10.5772/45627

Cited by 2 publications

(1 citation statement)

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“…So far, osteosarcoma therapy still combines some therapy modalities, including radiation therapy, immunotherapy, chemotherapy which uses chemotherapeutic agent, biological agent or neoadjuvant therapy, and surgical treatment including limb salvage and amputation (Jaffe et al 2013). However, these therapies are high costs and can cause side effects, such as pain and nausea, and may also affect the healthy cells (Agarwal and Nayak 2012;Chen et al 2013;Dicarpio et al 2003;Hutagalung 2005;Harisson et al 2018;Jaffe et al 2013;Yang 2005).…”

Section: Introductionmentioning

confidence: 99%

Non-contact Electric Field Exposure Provides Potential Cancer Therapy through p53-Independent Proliferation Arrest and Intrinsic Pathway Apoptosis Induction in MG-63 Cell Lines

et al. 2023

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Osteosarcoma is a highly malignant primary tumor on bone that mainly attacks children and young adolescents. Until now, osteosarcoma therapy still combines some high costs and invasive therapy modalities that may give side effects, such as pain and nausea. Our previous studies suggested that non-contact electric field has anti-proliferative effect on breast cancer cells, in vitro and in vivo. In this study, we were interested studying alternating current electric field effects on osteosarcoma cells progression as well as its potential cytotoxic effects. MG-63 human osteosarcoma cells were cultured and treated with 200 kHz for 6 days. Several genes of interest including p53, p21, MDM2, caspase-3, caspase-8, and caspase-9 were analyzed using real-time qPCR method. Apoptotic index was measured using flow-cytometry assay. Apoptosis was observed through p53-independent p21 pathway (p = 0.011). Cells undergoing apoptosis through internal pathways were shown by the increase of caspase-3 (p = 0.015) and caspase-9 (p = 0.001) levels, but not caspase-8 (p = 0.080). This treatment has successfully reduced the number of living osteosarcoma cells by 14.7% (p = 0.000) and increased cell death up to 4.26% (p = 0.055). Apoptotic index was markedly increased to 16% (p = 0.001). 200 kHz non-contact electric field exposure can disrupt osteosarcoma progression through disruption of normal cell cycle via p53-independent p21 pathway and induction of apoptosis.

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