1989
DOI: 10.1007/bf00270776
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Limitations during hydroxybutyrate converison to isoleucine with Corynebacterium glutamicum, as analysed by the formation of by-products

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Cited by 13 publications
(4 citation statements)
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“…All 20 proteins were individually assayed with amino donor L-Glu, L-Ala, L-Asp, or L-Gln using as the amino acceptor 2-oxo-3-methylvalerate (O-Ile), 2-oxo-isocaproate (O-Leu), or 2-oxo-isovalerate (O-Val). In addition, the L-Ile intermediate 2-oxo-butyrate (O-But) was assayed, which is known to be formed during L-Ile production (38). Product formation was followed over time, and the results where detectable amino acid formation occurred are shown in Table 2.…”
Section: Resultsmentioning
confidence: 99%
“…All 20 proteins were individually assayed with amino donor L-Glu, L-Ala, L-Asp, or L-Gln using as the amino acceptor 2-oxo-3-methylvalerate (O-Ile), 2-oxo-isocaproate (O-Leu), or 2-oxo-isovalerate (O-Val). In addition, the L-Ile intermediate 2-oxo-butyrate (O-But) was assayed, which is known to be formed during L-Ile production (38). Product formation was followed over time, and the results where detectable amino acid formation occurred are shown in Table 2.…”
Section: Resultsmentioning
confidence: 99%
“…The fact that this depends on ilvA greatly restricts the number of intermediates which can be considered. A detailed analysis of by-products, as was done for L-isoleucine synthesis from precursors (44), could help to define new limitations in current L-isoleucine producers and enable a new coordination of activities to be made between the metabolite-supplying and -consuming reactions within the entire reaction sequence of this industrially important amino acid.…”
Section: Discussionmentioning
confidence: 99%
“…To overcome some of these problems with the long and tightly controlled reaction sequence, processes have been used in which precursors are applied to channel the C 4 skeleton required for L-isoleucine synthesis directly into the final part of the pathway (4,21,40,44). Some attempts by classical techniques to derive L-isoleucine directly from sugar have also been made, since that would be more economical.…”
mentioning
confidence: 99%
“…A number of reports on L-isoleucine production by fermentation have been published (Eggeling and Sahm 1987;Ikeda et al 1976;Kisumi et al 1971a, b, Shiio et al 1973Wilhelm et al 1989;Yukawa et al 1986). However, this process has some disadvantages because of high carbon consumption during cell growth, a low conversion rate from the carbon source to the final product, and high concentrations of by-products.…”
Section: Introductionmentioning
confidence: 99%