Limits of detection for time of flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy (XPS): detection of low amounts of adsorbed protein

Wagner, Mary B.; McArthur, Sally L.; Shen, Mingchao; Horbett, Thomas A.; Castner, David G.

doi:10.1163/156856202320253938

Cited by 149 publications

(87 citation statements)

References 37 publications

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“…The XPS spectra of the pure fibrinogen multilayer film are consistent with those reported previously (41), showing multiple C(1s) peaks and significant intensity in the N(1s) and S(2p) regions. The C(1s) peak at 288.2 eVarises from carbon atoms in the amide groups (41).…”

Section: Resultssupporting

confidence: 90%

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

Stavis

Clare

Butler

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Carbon is an extremely versatile family of materials with a wide range of mechanical, optical, and mechanical properties, but many similarities in surface chemistry. As one of the most chemically stable materials known, carbon provides an outstanding platform for the development of highly tunable molecular and biomolecular interfaces. Photochemical grafting of alkenes has emerged as an attractive method for functionalizing surfaces of diamond, but many aspects of the surface chemistry and impact on biological recognition processes remain unexplored. Here we report investigations of the interaction of functionalized diamond surfaces with proteins and biological cells using X-ray photoelectron spectroscopy (XPS), atomic force microscopy, and fluorescence methods. XPS data show that functionalization of diamond with short ethylene glycol oligomers reduces the nonspecific binding of fibrinogen below the detection limit of XPS, estimated as >97% reduction over H-terminated diamond. Measurements of different forms of diamond with different roughness are used to explore the influence of roughness on nonspecific binding onto H-terminated and ethylene glycol (EG)-terminated surfaces. Finally, we use XPS to characterize the chemical stability of Escherichia coli K12 antibodies on the surfaces of diamond and amine-functionalized glass. Our results show that antibody-modified diamond surfaces exhibit increased stability in XPS and that this is accompanied by retention of biological activity in cell-capture measurements. Our results demonstrate that surface chemistry on diamond and other carbonbased materials provides an excellent platform for biomolecular interfaces with high stability and high selectivity.biointerfaces | surface chemistry | cells

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Section: Resultssupporting

confidence: 90%

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

Stavis

Clare

Butler

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…On the other hand, super-hydrophilic surface of titanium dioxide films 33 or O 2 -treated HMDSO films that showed a high rate of cell attachment had a rigid surface. It is also necessary to investigate the protein conformation that influences cell adhesion by using a TOF-SIMS system 29,30,34 for better understanding of these phenomena.…”

Section: Discussionmentioning

confidence: 99%

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

et al. 2006

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Surface wettability is an important physicochemical property of biomaterials, and it would be more helpful for understanding this property if a wide range of wettability are employed. This study focused on the effect of surface wettability on fibroblast adhesion over a wide range of wettability using a single material without changing surface topography. Plasma polymerization with hexa methyldisiloxane followed by oxygen (O 2 ) plasma treatment was employed to modify the surfaces. The water contact angle of sample surfaces varied from 106 degrees (hydrophobicity) to almost 0 degrees (super-hydrophilicity). O 2 -functional groups were introduced on polymer surfaces during O 2 -plasma treatment. The cell attachment study confirmed that the more hydrophilic the surface, the more fibroblasts adhered in the initial stage that includes on super-hydrophilic surfaces. Cells spread much more widely on the hydrophilic surfaces than on the hydrophobic surfaces. There was no significant difference in fibroblast proliferation, but cell spreading was much greater on the hydrophilic surfaces. These findings suggest the importance of the surface wettability of biomaterials on initial cell attachment and spreading. The degree of wettability should be taken into account when a new biomaterial is to be employed. Further research of surface wettability on adhesive molecules is necessary for a better understanding of this property.

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“…2 C-E). These ion clusters have previously been used as indicators for the presence of the amino acids methionine, C 5 H 11 NO 2 S, and threonine, C 4 H 9 NO 3 (20), but could arise from other organic molecules with a similar mass. Confocal micro-Raman spectroscopy corroborates the ToF-SIMS findings showing the presence of amide functional groups within the organic material as well as aliphatic and aromatic functional groups (Fig.…”

Section: Serpentinite Clasts From the South Chamorro Mud Volcanomentioning

confidence: 99%

Subduction zone forearc serpentinites as incubators for deep microbial life

Plümper

Geisler

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Serpentinization-fueled systems in the cool, hydrated forearc mantle of subduction zones may provide an environment that supports deep chemolithoautotrophic life. Here, we examine serpentinite clasts expelled from mud volcanoes above the Izu-BoninMariana subduction zone forearc (Pacific Ocean) that contain complex organic matter and nanosized Ni-Fe alloys. Using time-of-flight secondary ion mass spectrometry and Raman spectroscopy, we determined that the organic matter consists of a mixture of aliphatic and aromatic compounds and functional groups such as amides. Although an abiotic or subduction slab-derived fluid origin cannot be excluded, the similarities between the molecular signatures identified in the clasts and those of bacteria-derived biopolymers from other serpentinizing systems hint at the possibility of deep microbial life within the forearc. To test this hypothesis, we coupled the currently known temperature limit for life, 122°C, with a heat conduction model that predicts a potential depth limit for life within the forearc at ∼10,000 m below the seafloor. This is deeper than the 122°C isotherm in known oceanic serpentinizing regions and an order of magnitude deeper than the downhole temperature at the serpentinized Atlantis Massif oceanic core complex, MidAtlantic Ridge. We suggest that the organic-rich serpentinites may be indicators for microbial life deep within or below the mud volcano. Thus, the hydrated forearc mantle may represent one of Earth's largest hidden microbial ecosystems. These types of protected ecosystems may have allowed the deep biosphere to thrive, despite violent phases during Earth's history such as the late heavy bombardment and global mass extinctions.deep biosphere | serpentinization | subduction zone | forearc

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Limits of detection for time of flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy (XPS): detection of low amounts of adsorbed protein

Cited by 149 publications

References 37 publications

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

Surface functionalization of thin-film diamond for highly stable and selective biological interfaces

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

Subduction zone forearc serpentinites as incubators for deep microbial life

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