Linalool controls the viability of Escherichia coli by regulating the synthesis and modification of lipopolysaccharide, the assembly of ribosome, and the expression of substrate transporting proteins

Gao, Zhipeng; Jiang, Sifan; Zhong, Weiming; Liu, Ting; Guo, Jiajing

doi:10.1016/j.foodres.2022.112337

Cited by 13 publications

(4 citation statements)

References 54 publications

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“…In our ndings, linalool demonstrated signi cant antibacterial e cacy against both the E. coli D5 and the ATCC25922 strain, as indicated by their respective MICs and MBCs. These results align with those reported by Gao et al (2023), reinforcing linalool's potential as a promising natural antimicrobial agent.…”

Section: Linalool Implications On Eps In E Coli Bfssupporting

confidence: 91%

Linalool as a Potential Agent for Inhibiting Escherichia coli Biofilm Formation and Exopolysaccharide Production

Wang,

Zhang

et al. 2024

Preprint

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Linalool—a compound commonly found in various plant essential oils—exhibits multiple biological activities, encompassing anti-bacterial, inflammatory, analgesic, and antioxidant properties. However, the linalool implications on Escherichia coli (E. coli) biofilm (BF) remain underexplored. We proposed to ascertain the linalool implications on the development of E. coli BF and its extracellular polysaccharides, as well as to assess the implications of linalool on E. coli in both suspension and BF states. We discovered that the minimum biofilm inhibitory concentrations (MBICs) of linalool versus E. coli were twice as high as the lowest inhibitory concentrations. Linalool had a deadly impact on clinical E. coli strains obtained from cows with clinical endometritis, regardless of whether they were in a planktonic or BF condition. Linalool suppressed the BF development in a way that was dependent on the dosage, with an MBIC of 4 µL/mL. This was verified by the use of the crystal violet test and scanning electron microscopy. Moreover, the CCK-8 assay and confocal laser scanning microscopy (CLSM) manifested significant hindrances in live bacteria within the BF. The concentration of extracellular polymeric compounds in the E. coli BF was also mitigated. Furthermore, CLSM and RT-qPCR analysis confirmed that linalool (2 µL/mL) significantly suppressed exopolysaccharide (EPS) and the pgaABCD gene expression, regulating an essential exopolysaccharide expression in BF formation. These findings revealed that linalool effectively suppressed viable bacteria, EPS production, and E. coli BF formation, presenting a new method for preventing BF-related infections.

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Section: Linalool Implications On Eps In E Coli Bfssupporting

confidence: 91%

Linalool as a Potential Agent for Inhibiting Escherichia coli Biofilm Formation and Exopolysaccharide Production

Wang,

Zhang

et al. 2024

Preprint

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“…Gao et al illustrated that linalool caused significant damage to the cell wall/membrane of E . coli , resulting in the lysis and death of bacterial cells [12] . Gao et al demonstrated that linalool [14] , Changshan - huyou Y.B.…”

Section: Discussionmentioning

confidence: 99%

“…However, their overuse and abuse have resulted in antibiotic resistance among various aquatic pathogens [11] . Therefore, the increasing need for sustainable and eco-friendly strategies to combat saprolegnia infections has driven research into natural antimicrobial agents, due to their advantages in terms of avoiding antibiotic resistance issues, health safety, and ecological sustainability [12] .…”

Section: Introductionmentioning

confidence: 99%

“…Linalool, a monoterpene alcohol found in various EOs, has shown promising antimicrobial activity against a wide range of pathogens [13] . Our groups and other research teams have reported that linalool exhibits antimicrobial activity against bacteria such as Escherichia coli [12] , Listeria monocytogenes [14] , Elizabethkingia miricola , Streptococcus pyogenes [15] , Pseudomonas fragi [16] , staphylococcus aureus [17] , Brochothrix thermosphacta [18] and Aeromonas hydrophila [19] , as well as against oomycetes like Colletotrichum lagenarium [20] and Phytophthora capsici [21] , and fungi including Aspergillus flavus [22] , Penicillium citrinum , Chaetomium globosum [23] , Colletotrichum gloesporioides , Fusarium oxysporum [24] , Botrytis cinerea [25] and Candida albicans [26] . Although the antimicrobial activity of linalool has been extensively studied in vitro , the potential immune mechanism in vivo remains poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Linalool’s Multifaceted Antimicrobial Potential: Unveiling its Antimicrobial Efficacy and Immunomodulatory Role AgainstSaprolegnia parasitica

Tang,

Zhong,

Tang

et al. 2024

Preprint

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Saprolegnia parasitica(S.parasitica) is one of the most virulent oomycete species in freshwater aquatic environments, causing severe saprolegniasis and leading to significant economic losses in the aquaculture industry. Thus far, the prevention and control of saprolegniasis face a shortage of medications. Linalool, a natural antibiotic alternative found in various essential oils, exhibits promising antimicrobial activity against a wide range of pathogens. In this study, the specific role of linalool in protectingS.parasiticainfection at bothin vitroandin vivolevels was investigated. Linalool showed multifaceted anti-oomycetes potential by both of antimicrobial efficacy and immunomodulatory efficacy. Forin vitrotest, linalool exhibited strong anti-oomycetes activity and mode of action included: (1) Linalool disrupted the cell membrane of the mycelium, causing the intracellular components leak out; (2) Linalool prohibited ribosome function, thereby inhibiting protein synthesis and ultimately affecting mycelium growth. Surprisingly, meanwhile we found the potential immune protective mechanism of linalool in thein vivotest: (1) Linalool enhanced the complement and coagulation system which in turn activated host immune defense and lysateS.parasiticacells; (2) Linalool promoted wound healing, tissue repair, and phagocytosis to cope withS.parasiticainfection; (3) Linalool positively modulated the immune response by increasing the abundance of beneficial Actinobacteriota; (4) Linalool stimulated the production of inflammatory cytokines and chemokines to lyseS.parasiticacells. In all, our findings showed that linalool possessed multifaceted anti-oomycetes potential which would be a promising natural antibiotic alternative to cope withS.parasiticainfection in the aquaculture industry.

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In vitro and in vivo anti-oomycetes activities and mechanisms of linalool against Saprolegnia ferax

Tang,

Zhong,

Yang

et al. 2024

Aquaculture

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Linalool controls the viability of Escherichia coli by regulating the synthesis and modification of lipopolysaccharide, the assembly of ribosome, and the expression of substrate transporting proteins

Cited by 13 publications

References 54 publications

Linalool as a Potential Agent for Inhibiting Escherichia coli Biofilm Formation and Exopolysaccharide Production

Linalool as a Potential Agent for Inhibiting Escherichia coli Biofilm Formation and Exopolysaccharide Production

Linalool’s Multifaceted Antimicrobial Potential: Unveiling its Antimicrobial Efficacy and Immunomodulatory Role AgainstSaprolegnia parasitica

In vitro and in vivo anti-oomycetes activities and mechanisms of linalool against Saprolegnia ferax

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