LINC00844 promotes proliferation and migration of hepatocellular carcinoma by regulating NDRG1 expression

Abstract

Background Aberrant expression of long noncoding RNAs are implicated in the pathogenesis of human malignancies. LINC00844 expression is dramatically downregulated in prostate cancer, and functional studies have revealed the association between the aberrant expression of LINC00844 and prostate cancer cell invasion and metastasis. However, the function and mechanism of action of LINC00844 in the pathogenesis of hepatocellular carcinoma (HCC) are poorly understood. Methods LINC00844 and N-Myc downstream-regulated 1 (NDRG1) expression in HCC tissues and cell lines was detected with real-time quantitative polymerase chain reaction (RT-qPCR) and western blot analysis. Correlations between LINC00844 expression level and clinicopathological features were investigated using the original data from The Cancer Genome Atlas (TCGA) database. HepG2 and HCCLM9 cell lines were transfected with Lv-LIN00844 virus to obtain LINC00844-overexpressing cell lines. Cell proliferation and cell invasion and migration were examined with the cell counting kit-8 (CCK-8) and transwell assay, respectively. Furthermore, the correlation between LINC00844 and NDRG1 expression was analysed using Pearson’s correlation analysis. Results LINC00844 expression was significantly downregulatedin HCC tissues and cell lines, and a statistical correlation was detected between low LINC00844 expression and sex (Female), advanced American Joint Committee on Cancer (AJCC) stage (III + IV), histological grade (G3 + G4), and vascular invasion (Micro and Macro). In vitro experiments showed that LINC00844 overexpression significantly repressed the proliferation, migration, and invasion of HCC cells. NDRG1 expression was higher in HCC tissues and LINC00844 could partly inhibit the expression of NDRG1.

Long intergenic noncoding RNA 00844 promotes apoptosis and represses proliferation of prostate cancer cells through upregulating GSTP1 by recruiting EBF1

“…Notably, an existing study also acknowledged the profound inhibitory effects of LINC00844 in malignant and metastatic PC by preventing migration and invasion (Lingadahalli et al, 2018), which further confirmed our findings to classify LINC00844 as a tumor suppressor. Although in the prior study, the authors claimed that LINC00844 did not extensively affect PC cell proliferation, our findings suggested that (Zhou et al, 2020). Therefore, it can be ascertained that LINC00844 exercises a repressing effect on PC progression based on the aforementioned findings.…”

Identification of a Glycolysis-Related LncRNA Signature to Predict Survival in Diffuse Glioma Patients

“…In the signature, we identified six LncRNAs (FOXD2-AS1, AC062021.1, AF131216.5, LINC00844, CRNDE and LINC00665). FOXD2-AS1 (32, 33), LINC00844 (34,35), CRNDE (5,6), and LINC00665 (36,37) were reported to have the similar biological functions and to be associated with prognosis, cell proliferation, invasion, cell cycle, and metastasis. Meanwhile, as demonstrated by existing reports, glycolysis activity has implicated in the cell proliferation (9-11), cytotoxic activity (38), immune response (39,40), drug resistance (41,42), poor prognosis (43)(44)(45)(46), and tumor microenvironment (47) in most cancers.…”

TPRG1‐AS1 induces RBM24 expression and inhibits liver cancer progression by sponging miR‐4691‐5p and miR‐3659

“…We observed that most of these ncRNAs (37/39) were downregulated in the aggressive subtype CC2, implying their tumour‐suppressive functions (Figure 2A). Indeed, LINC00844 , the lowest‐ranked ncRNA in CC2, has been reported to have a tumour‐suppressive function in HCC and other cancer types 13,14 . By contrast, HAGLR ( HOXD‐AS1 ), the top‐ranked ncRNA in CC2, has been reported to promote HCC metastasis 15 .…”