Lindane Biodegradation by Defined Consortia of Indigenous Streptomyces Strains

Abstract

The current study aimed to compare lindane degradation by pure and mixed cultures of Streptomyces sp. Cell-free extracts were assayed for potentiating dechlorinase activity and, based on these results, consortia of two to six microorganisms were assayed for their growth on and degradation of lindane. Furthermore, the role of bacterial consortia of lindane-degrading strains was examined in lindane decontamination soil assays. Four actinobacteria, previously isolated from a pesticide-contaminated area, were selected because of their tolerance to lindane and their ability to use the pesticide as sole carbon source. These strains as well as Streptomyces sp. M7 and Streptomyces coelicolor A3 were used to study specific dechlorinase activity (SDA) and lindane removal in mixed cultures. Pure cultures presented SDA in the presence of 1.66 mg L -1 lindane as carbon source. SDA was improved by certain mixed cultures until 12 times compared with pure cultures. Mixed cultures with two, three, and four strains showed maximum lindane removal of 46% to 68%, whereas combinations of five and six strains did not efficiently remove the pesticide from the culture medium. The Streptomyces sp. A2, A5, M7, and A11 consortium presented the lowest ratio between residual lindane concentration and SDA and could be a promising tool for lindane biodegradation.

Versatility of Streptomyces sp. M7 to bioremediate soils co-contaminated with Cr(VI) and lindane

“…Extracts were quantified in a Gas Chromatograph Agilent 7890 A equipped with a HP5 capillary column (30 m  0.53 mm  0.35 m) and 63 Ni μECD detector, a split/splitless Agilent 7693B injector and Agilent Chem Station software. Quantitative sample analysis was performed using appropriate calibration standards (AccuStandard) (Fuentes et al, 2011).…”

“…Cr(VI) is a harmful pollutant, neurotoxic, dermatotoxic, genotoxic, carcinogenic and immunotoxic (Bagchi et al, 2002). On the other hand, residues of the gamma isomer of hexachlorocyclohexane (γ-HCH), commercially known as lindane, have been reported in soils, water, air, plants and animals, because of its indiscriminate use, principally in agriculture practices (Fuentes et al, 2011). Lindane is highly recalcitrant, and produces several health effects, such as neurological problems and cancer .…”

Methoxychlor bioremediation by defined consortium of environmental Streptomyces strains

“…These microorganisms have a great potential for the biodegradation of organic and inorganic toxic compounds, and several studies have demonstrated oxidation and partial dechlorination and dealkylation of aldrin, DDT and herbicides like metolachlor and atrazine, particularly in Streptomyces (Liu et al 1990;Radosevich et al 1995), the main genus of actinobacteria present in soils (Xu et al 1996). In previous studies, Benimeli et al (2003Benimeli et al ( , 2006Benimeli et al ( , 2007 and Fuentes et al (2010Fuentes et al ( , 2011 isolated and selected wild-type Streptomyces spp. strains which were able to tolerate and remove lindane (c-hexachlorocyclohexane), another OP, from culture media and soil, as pure and mixed cultures.…”

Degradation study of lindane by novel strains Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W

“…M716.6 mg/LSineli et al (2016)3. Sphingomonas baderi sp.100 mg/LKaur et al (2013)4. Arthrobacter florescens and Arthrobacter giacomelloi 100 mg/LDe Polis et al (2013)5. Actinobacteria sp. and Streptomyces sp.1.66 mg/L optimum for degradationFuentes et al (2010, 2011)6. Kocuria rhizophila, Microbacterium resistens, Staphylococcus equorum, Staphylococcus cohnii subsp.ureolyticus 5–100 μg/mLAbhilash et al (2011)7.Lindane-degrading consortia300 μg/mL after 108 hElcey and Kunhi (2010)8. Azotobacter chroococcum JL102100 ppmAnupama and Paul (2010)9. Sphingomonas sp. NM05100 μg/mLManickam et al (2008)10. Sphingomonas sp.…”