Lineage-specific and differentiation-dependent expression of K12 keratin in rabbit corneal/limbal epithelial cells: cDNA cloning and Northern blot analysis

Wu, Ren‐Long; Zhu, Guang; Galvin, Sharon; Xu, Cong; Haseba, Takeshi; Chaloin-Dufau, Catherine; Dhouailly, Danielle; Wei, Zhi; Lavker, Robert M.; Kao, Winston W.‐Y.; Sun, Tung Tien

doi:10.1046/j.1432-0436.1994.5520137.x

Cited by 38 publications

(14 citation statements)

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“…The specific expression of K3 and K12 within the cornea (Liu et al, 1993 ;Moll et al, 1982 ;Sun et al, 1984 ;Wu et al, 1994), explains why this is the only tissue affected by mutations within these keratin genes. However, corneal fragility has also been observed in patients with EBS (Adamis, Schein and Kenyon, 1993 ;Granek and Baden, 1980 ;Lin et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…The keratin filament networks of corneal keratinocytes are less tightly bundled compared to the keratin networks of other stratified epithelia (Kao et al, 1996). Corneal keratinocytes specifically express keratins K3 and K12 (Liu et al, 1993 ;Moll et al, 1982 ;Sun et al, 1984 ;Wu et al, 1994), which are regarded as markers of advanced corneal epithelial differentiation (Rodrigues et al, 1987 ;Schermer, Galvin and Sun, 1986). K3 and K12 are found in suprabasal cells of the limbus and all epithelial cell layers in the centre of the cornea (Liu et al, 1994 ;Schermer, Galvin and Sun, 1986 ;Wu et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…Corneal keratinocytes specifically express keratins K3 and K12 (Liu et al, 1993 ;Moll et al, 1982 ;Sun et al, 1984 ;Wu et al, 1994), which are regarded as markers of advanced corneal epithelial differentiation (Rodrigues et al, 1987 ;Schermer, Galvin and Sun, 1986). K3 and K12 are found in suprabasal cells of the limbus and all epithelial cell layers in the centre of the cornea (Liu et al, 1994 ;Schermer, Galvin and Sun, 1986 ;Wu et al, 1994). K3 and K12 are not found in the conjunctiva (Chaloin-Dufau, Sun and Dhouailly, 1990 ;Liu et al, 1993 ;Schermer, Galvin and Sun, 1986).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Genetics of Meesmann's Corneal Dystrophy: Ancestral and Novel Mutations in Keratin 12 (K12) and Complete Sequence of the Human KRT12 Gene

Corden

Swensson

et al. 2000

Experimental Eye Research

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Genetics of Meesmann's Corneal Dystrophy: Ancestral and Novel Mutations in Keratin 12 (K12) and Complete Sequence of the Human KRT12 Gene

Corden

Swensson

et al. 2000

Experimental Eye Research

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“…Ablation of Srf restores normal epithelial differentiation in Dstn corn1 cornea: In the normal cornea, differentiated corneal epithelial cells express keratin 12 (Krt12) (Liu et al 1993;Wu et al 1994), and populate almost the entire corneal epithelium with the exception of basal cells in the limbal (peripheral) epithelium (Liu et al 1993). This widespread KRT12 expression was found to be affected in Dstn corn1 mice in a previous study (Zhang et al 2008) and is markedly lost by P58 in Dstn corn1/corn1 control mice (Figure 7), denoting a problem with epithelial cell identity in the cornea.…”

Section: /Srfmentioning

confidence: 99%

A Pathogenic Relationship Between a Regulator of the Actin Cytoskeleton and Serum Response Factor

et al. 2010

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Cell hyperproliferation, inflammation, and angiogenesis are biological processes central to the pathogenesis of corneal disease, as well as other conditions including tumorigenesis and chronic inflammatory disorders. Due to the number of disease conditions that arise as a result of these abnormalities, identifying the molecular mechanisms underlying these processes is critical. The avascular and transparent cornea serves as a good in vivo model to study the pathogenesis of cell hyperproliferation, inflammation, and angiogenesis. Corneal disease 1 (Dstn corn1 ) mice are homozygous for a spontaneous null allele of the destrin (Dstn) gene, which is also known as actin depolymerizing factor (ADF). These mice exhibit abnormalities in the cornea including epithelial cell hyperproliferation, stromal inflammation, and neovascularization. We previously identified that the transcription factor, serum response factor (SRF) and a number of its target genes are upregulated in the cornea of these mice. In this study, we show that conditional ablation of Srf in the corneal epithelium of a diseased Dstn corn1 cornea results in the rescue of the epithelial cell hyperproliferation, inflammation, and neovascularization phenotypes, delineating an epithelial cell-specific role for SRF in the development of all of these abnormalities. Our study also demonstrates that Dstn is genetically upstream of Srf and defines a new functional role for SRF as the master regulator of a hyperproliferative, inflammatory phenotype accompanied by neovascularization.

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“…The current dogma is that the expression of K3/12 is thought to be a hallmark of epithelia with cornea-type differentiation 1,[15][16][17][18][19][20] and to be indispensable for corneal epithelial homeostasis. 21 However, K3 is also expressed in other epithelia, including snout, 16 gingiva, and tongue 22 and palpebral conjunctiva.…”

mentioning

confidence: 99%

Clusters of Corneal Epithelial Cells Reside Ectopically in Human Conjunctival Epithelium

Kawasaki

Tanioka

Yamasaki

et al. 2006

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.The ocular surface is covered by two biologically distinct epithelia: corneal and conjunctival. The expression of keratin12 (K12) is currently considered a hallmark of corneatype differentiation. In the current study, the biological features of K12-positive cells in human bulbar conjunctival epithelium were examined. METHODS. Human conjunctival tissues were subjected to investigate the K12-positive cells in conjunctiva by immunostaining, in situ hybridization, Western blot analysis, reverse transcriptase-polymerase chain reaction (RT-PCR), and fluorescenceactivated cell sorting (FACS). Gene expression profiling of these cells was performed with introduced amplified-fragment length polymorphism (iAFLP). To determine the presence of stem-or progenitor cells, immunostaining and colony-forming assays were performed. RESULTS. Western blot analysis, RT-PCR revealed that K12 was expressed in conjunctival epithelium. Immunostaining analysis showed that K12-positive cells reside mainly in clusters in conjunctival epithelium. FACS analysis showed that 0.2% to 1.7% of conjunctival epithelial cells collected from the inferior bulbar conjunctiva were K12 positive. iAFLP analysis revealed that the gene expression patterns of these cells were highly similar to that of corneal epithelial cells. p63 and ABCG2 were expressed beneath the K12-positive cells. Some colony-forming cells expressed K12.

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Lineage-specific and differentiation-dependent expression of K12 keratin in rabbit corneal/limbal epithelial cells: cDNA cloning and Northern blot analysis

Cited by 38 publications

References 33 publications

Molecular Genetics of Meesmann's Corneal Dystrophy: Ancestral and Novel Mutations in Keratin 12 (K12) and Complete Sequence of the Human KRT12 Gene

Molecular Genetics of Meesmann's Corneal Dystrophy: Ancestral and Novel Mutations in Keratin 12 (K12) and Complete Sequence of the Human KRT12 Gene

A Pathogenic Relationship Between a Regulator of the Actin Cytoskeleton and Serum Response Factor

Clusters of Corneal Epithelial Cells Reside Ectopically in Human Conjunctival Epithelium

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