Lineage tracing: technology tool for exploring the development, regeneration, and disease of the digestive system

Zhang, Yue; Zeng, Fanhong; Han, Xu; Weng, Jun; Gao, Yi

doi:10.1186/s13287-020-01941-y

Cited by 13 publications

(12 citation statements)

References 134 publications

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“…This ectopic expression can sometimes lead to genetic labeling, which is unwanted and unintentional tracing, and may lead to misinterpretation of some cell fate studies [ 142 ]. In addition, Cre recombinase may also cause gene mutations, thus inducing developmental abnormalities or even embryonic lethality, and tamoxifen may cause off-target effects, e.g., damaging the gastric mucosa [ 143 ]. In order to circumvent these limitations, novel more complex modes of lineage tracing were developed [ 144 , 145 ].…”

Section: Mechanisms Responsible For Cell Faith Change During Liver Ca...mentioning

confidence: 99%

Current view of liver cancer cell-of-origin and proposed mechanisms precluding its proper determination

2023

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Hepatocellular carcinoma and intrahepatic cholangiocarcinoma are devastating primary liver cancers with increasing prevalence in many parts of the world. Despite intense investigation, many aspects of their biology are still largely obscure. For example, numerous studies have tackled the question of the cell-of-origin of primary liver cancers using different experimental approaches; they have not, however, provided a clear and undisputed answer. Here, we will review the evidence from animal models supporting the role of all major types of liver epithelial cells: hepatocytes, cholangiocytes, and their common progenitor as liver cancer cell-of-origin. Moreover, we will also propose mechanisms that promote liver cancer cell plasticity (dedifferentiation, transdifferentiation, and epithelial-to-mesenchymal transition) which may contribute to misinterpretation of the results and which make the issue of liver cancer cell-of-origin particularly complex.

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Section: Mechanisms Responsible For Cell Faith Change During Liver Ca...mentioning

confidence: 99%

Current view of liver cancer cell-of-origin and proposed mechanisms precluding its proper determination

2023

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“…3l, Supplementary Fig. S4d ) 25 . This observation confirms the preservation of this “conveyor belt” mechanism in our model system.…”

Section: Resultsmentioning

confidence: 99%

A Bioengineered Platform for Enhanced Observability of Patterned Gastrointestinal Organoid Monolayers with Bilateral Access

Hofer,

Duque-Correa,

Lutolf

2024

Preprint

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Studying the physiology and pathology of gastrointestinal (GI) tissues requires tools that can accurately mimic their complex architecture and functionality in vitro. Organoids have emerged as one such promising tool, though their closed structures with poorly accessible lumen and limited observability makes readouts challenging. In this study, we introduce a bioengineered organoid platform that generates bilaterally accessible 3D tissue models, allowing independent manipulation of both the apical and basal sides of patterned epithelial monolayers. We successfully constructed gastric, small intestinal, caecal, and colonic epithelial models that faithfully reproduce tissue-respective geometries and exhibit high physiological relevance, evidenced by the regionalization of stem cells and transcriptional resemblance to real epithelia. The gained observability allowed single-cell tracking over time and studies into the motility of cells in immersion and air-liquid interface cultures. Additionally, this model recapitulated Trichuris muris infection of the caecum epithelium, allowing the first live imaging of syncytial tunnel formation. Overall, this platform offers accessible organoids with improved observability, making it a valuable tool for investigating the dynamics of GI epithelial cells and their interactions with pathogens

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“…85,86 Determining the fate of stem cells and what influences them has important therapeutic implications, and allows more in-depth understanding of organ development. 85,87,88 These principles can be applied to monitor the effects of new drugs to improve quality control, and monitor disease progress to develop drugs in the first place.…”

Section: Cell Lineage Tracingmentioning

confidence: 99%

Cellular Surveillance: DNA-Based Recording to Monitor and Memorize Biological Events

2023

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Continuous monitoring of intra-and extra-cellular environments, ranging from open fields and rivers to within a single cell, is often limited by costly equipment, labour intensity or invasive techniques. It may, however, be possible to avoid these issues by developing a monitoring device on a much smaller scale. By applying novel synthetic biology techniques, it is now possible to engineer organisms with refined bio-recording capabilities, using DNA as a recording medium on which a cellular 'memory' of past events can be written. Through a variety of mechanisms, using recombinases, CRISPR nucleases, base editing, polymerases and prime editing, intra-and extracellular events can be recorded along with temporal, spacial and magnitude information.Here, the mechanisms developed for DNA-based bio-recording devices so far are assessed and discussed, within the context of the potential future applications of these devices.

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Lineage tracing: technology tool for exploring the development, regeneration, and disease of the digestive system

Cited by 13 publications

References 134 publications

Current view of liver cancer cell-of-origin and proposed mechanisms precluding its proper determination

Current view of liver cancer cell-of-origin and proposed mechanisms precluding its proper determination

A Bioengineered Platform for Enhanced Observability of Patterned Gastrointestinal Organoid Monolayers with Bilateral Access

Cellular Surveillance: DNA-Based Recording to Monitor and Memorize Biological Events

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