Linear correlation between inactivation of E. coli and OH radical concentration in TiO2 photocatalytic disinfection

Cho, Min; Chung, Hyen-Mi; Choi, Wonyong; Yoon, Jeyong

doi:10.1016/j.watres.2003.10.029

Cited by 734 publications

(496 citation statements)

References 24 publications

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“…Briefly, 1.8 mL of samples were mixed with 0.2 mL 100 mg L À1 methyl orange, and the pH was adjusted to 2.5 with 0.1 M H 2 SO 4 . (Cho et al, 2004): and [FFA] was determined by HPLC at 218 nm, and the mobile phase was methanol:H 2 O ¼ 20:80 at 0.8 mL min À1 .…”

Section: Ross Detection and The Enzymatic Activities Assaymentioning

confidence: 99%

Unveiling the photoelectrocatalytic inactivation mechanism of Escherichia coli : Convincing evidence from responses of parent and anti-oxidation single gene knockout mutants

Sun

et al. 2016

Water Research

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Section: Ross Detection and The Enzymatic Activities Assaymentioning

confidence: 99%

Unveiling the photoelectrocatalytic inactivation mechanism of Escherichia coli : Convincing evidence from responses of parent and anti-oxidation single gene knockout mutants

Sun

et al. 2016

Water Research

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“…can cause decomposition of bacteria through various actions [16,17]. In biological systems, antimicrobial action of ROS is achieved by lipid peroxidation of the cell membrane and subsequent inactivation of the microorganisms [18].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial properties of F-doped ZnO visible light photocatalyst

Podporska-Carroll

Myles

Quilty

et al. 2017

Journal of Hazardous Materials

208

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“…Some studies have focused on photocatalytic disinfection using TiO 2 powder as an additive, not only for determination of reaction parameters (TiO 2 concentration, light intensity, pH, etc. ), but also to understand the mechanism of microorganism inactivation with this process (Cho et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

Degradation and inactivation of adenovirus in water by photo-electro-oxidation

et al. 2015

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The present study analyzed the efficiency of the photo-electro-oxidation process as a method for degradation and inactivation of adenovirus in water. The experimental design employed a solution prepared from sterile water containing 5.10 7 genomic copies/L (gc/L) of a standard strain of human adenovirus type 5 (HAdV-5) divided into two equal parts, one to serve as control and one treated by photo-electro-oxidation (PEO) for 3 hours and with a 5A current. Samples collected throughout the exposure process were analyzed by real-time polymerase chain reaction (qPCR) for viral genome identification and quantitation. Prior to gene extraction, a parallel DNAse treatment step was carried out to assess the integrity of viral particles. Integrated cell culture (ICC) analyses assessed the viability of infection in a cell culture. The tested process proved effective for viral degradation, with a 7 log 10 reduction in viral load after 60 minutes of treatment. The DNAse-treated samples exhibited complete reduction of viral load after a 75 minute exposure to the process, and ICC analyses showed completely non-viable viral particles at 30 minutes of treatment.Keywords: adenovirus, advanced oxidation process, photo-electro-oxidation, water. Degradação e inativação de adenovírus na água por fotoeletrooxidação ResumoO presente estudo analisou a eficiência do processo de fotoeletrooxidação como metodologia para a degradação e inativação de adenovírus em água. A concepção experimental emprega uma solução preparada a partir de água estéril contendo 5,10 7 cópias genômicas/L (gc/L) de uma amostra padrão de adenovírus humano tipo 5 (HAdV-5), dividida em duas partes iguais, uma para servir como controle e outra tratada por fotoeletrooxidação (PEO) durante 3 horas e com uma corrente de 5A. As amostras recolhidas durante o processo de exposição foram analisadas por PCR quantitativo em tempo real (qPCR) para identificação e quantificação do genoma viral. Antes da extração de ácidos nucleicos, um passo de tratamento com DNAse paralelo foi realizado para avaliar a integridade das partículas virais. Um ensaio de qPCR integrado à cultura de células (ICC-qPCR) permitiu analisar a viabilidade de infecção em uma cultura de células. O processo mostrou-se eficaz testada para a degradação viral, com uma redução de 7 log 10 da carga viral após 60 minutos de tratamento. As amostras tratadas com DNAse exibiram redução completa da carga viral após uma exposição de 75 minutos ao processo, e a análise de ICC-qPCR mostrou partículas virais completamente não-viáveis em 30 minutos de tratamento.Palavras-chave: adenovírus, processo de oxidação avançada, fotoeletrooxidação, água.

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Linear correlation between inactivation of E. coli and OH radical concentration in TiO2 photocatalytic disinfection

Cited by 734 publications

References 24 publications

Unveiling the photoelectrocatalytic inactivation mechanism of Escherichia coli : Convincing evidence from responses of parent and anti-oxidation single gene knockout mutants

Unveiling the photoelectrocatalytic inactivation mechanism of Escherichia coli : Convincing evidence from responses of parent and anti-oxidation single gene knockout mutants

Antibacterial properties of F-doped ZnO visible light photocatalyst

Degradation and inactivation of adenovirus in water by photo-electro-oxidation

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