Linezolid Versus Vancomycin for the Treatment of Methicillin-Resistant Staphylococcus aureus Keratitis in Rabbits

Abstract

Topical LZD showed activity against MRSA that was comparable to fortified VA in this experimental keratitis model.

Vancomycin-loaded niosomes integrated within pH-sensitive in-situ forming gel for treatment of ocular infections while minimizing drug irritation

“…The in‐vivo antimicrobial efficacy was studied in experimental infection model using 15 female albino rabbits divided into three groups (five animals per group, each weighing 2–3 kg), as described previously . One hundred CFU of MRSA bacteria were injected intrastromally into the rabbit corneas.…”

Efficacy of Daptomycin on Experimental Methicillin-Resistant Staphylococcus aureus Keratitis in Rabbits

“…21 Since there are no susceptibility standards for interpreting bacterial susceptibility in ocular infections, the MIC and disc susceptibility criteria for systemic administration are used to select the antimicrobial agent to treat bacterial keratitis. 10,22,23 However, some reports provide evidence of a relationship between the MIC values of topically applied agents and clinical outcomes in cases of bacterial keratitis. 24,25 Therefore, the MIC value of an agent is accepted to be an important measure for evaluating the efficacy of topically applied antimicrobials in the treatment of bacterial keratitis.…”

“…The final score for each eye represented the average of the scores of the observers. 3,10 Treatment protocol Treatment began immediately after the first examination and scoring, which were completed 16 h after injection. The rabbits were randomized into 4 treatment groups (7 rabbits per group) by an investigator who was not involved in the examination and scoring.…”

“…One drop was applied to each eye every 15 min for the first 5 doses and then every 30 min for the remaining 14 doses (19 doses in total over 8.25 h). 3,10 Euthanasia and tissue harvesting After the treatment and the final slit-lamp examination, the rabbits were euthanized with an intravenous overdose of sodium pentobarbital (100 mg/kg; Sigma-Aldrich) at 25 h postinfection. Rabbit corneas were excised using a uniform sterile 7.5-mm Hessburg-Barron vacuum trephine (Altomed).…”

“…Bilateral infection in the central cornea does not produce a level of visual disability sufficient to disrupt the normal daily activity. 3,10 Preparation of the MRSA strain An ATCC MRSA 43300 standard strain was used for the keratitis model. A frozen stock of the isolate was inoculated onto blood agar and was incubated overnight at 37°C.…”