Linkage Map for the Asian Tiger Mosquito [Aedes (Stegomyia) albopictus] Based on SSCP Analysis of RAPD Markers

Mutebi, John-Paul; Black, William C.; Bosio, Christopher F.; Sweeney, W. P.; Craig, George B.

doi:10.1093/oxfordjournals.jhered.a023142

Cited by 20 publications

(14 citation statements)

References 39 publications

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“…Severe distortion was expected for chromosome 1 due to autosomal sex determination in Ae. albopictus (Mutebi et al, 1997). Transmission distortion for chromosomes 2 and 3 was predominantly due to a deficit in Tok-au homozygotes; however, all expected genotypic classes were represented at each locus.…”

Section: Dna Extraction and Genotypingmentioning

confidence: 92%

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Quantitative trait loci determining autogeny and body size in the Asian tiger mosquito (Aedes albopictus)

et al. 2008

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Section: Dna Extraction and Genotypingmentioning

confidence: 92%

“…As sex determination in Ae. albopictus is controlled by a single autosomal locus on chromosome 1 (Mutebi et al, 1997), few paternal homozygotes were obtained for chromosome 1 markers (see Severson et al, 1993).…”

Section: Linkage Analysismentioning

confidence: 99%

Quantitative trait loci determining autogeny and body size in the Asian tiger mosquito (Aedes albopictus)

et al. 2008

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“…darlingi 13 and other species of mosquitoes 21 and as a tool to construct genetic maps. 22 In this study, we present a new PCR-based assay that permits a simple and low cost identification of An. darlingi.…”

Section: Introductionmentioning

confidence: 99%

Limited Diversity of Anopheles Darlingi in the Peruvian Amazon Region of Iquitos

Pinedo-Cancino¹,

Sheen²,

Tarazona‐Santos³

et al. 2006

The American Journal of Tropical Medicine and Hygiene

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Abstract. Anopheles darlingi is the most important malaria vector in the Amazon basin of South America, and is capable of transmitting both Plasmodium falciparum and P. vivax. To understand the genetic structure of this vector in the Amazonian region of Peru, a simple polymerase chain reaction (PCR)-based test to identify this species of mosquito was used. A random amplified polymorphic DNA-PCR was used to study genetic variation at the micro-geographic level in nine geographically separate populations of An. darlingi collected in areas with different degrees of deforestation surrounding the city of Iquitos. Within-population genetic diversity in nine populations, as quantified by the expected heterozygosity (H E ), ranged from 0.27 to 0.32. Average genetic distance (F ST ) among these populations was 0.017. These results show that the nine studied populations are highly homogeneous, suggesting that strategies can be developed to combat this malaria vector as a single epidemiologic unit.

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“…We have used this region in population genetic analyses of a number of arthropod species (27,(81)(82)(83). There is a large amount of variation in this gene within a species and the distribution of alleles fits the infinite allele model, suggesting that it is selectively neutral.…”

Section: Population Genetic Studiesmentioning

confidence: 99%

Molecular and genetic ecotoxicologic approaches to aquatic environmental bioreporting.

Beaty

Black

Carlson

et al. 1998

Environ Health Perspect

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Molecular and population genetic ecotoxicologic approaches are being developed for the utilization of arthropods as bioreporters of heavy metal mixtures in the environment. The explosion of knowledge in molecular biology, molecular genetics, and biotechnology provides an unparalleled opportunity to use arthropods as bioreporter organisms. Interspecific differences in aquatic arthropod populations have been previously demonstrated in response to heavy metal insult in the Arkansas River (AR) California Gulch Superfund site (CGSS). Population genetic analyses were conducted on the mayfly Baetis tricaudatus. Genetic polymorphisms were detected in polymerase chain reaction amplified 16S mitochondrial rDNA (a selectively neutral gene) of B. tricaudatus using single-strand conformation polymorphism analysis. Genetic differences may have resulted from impediments to gene flow in the population caused by mortality arising from exposure to heavy metal mixture pollution. In laboratory studies a candidate metal-responsive mucinlike gene, which is metal and dose specific, has been identified in Chironomus tentans and other potential AR-CGSS bioreporter species. Population genetic analyses using the mucinlike gene may provide insight into the role of this selectable gene in determining the breeding structure of B. tricaudatus in the AR-CGSS and may provide mechanistic insight into determinants of aquatic arthropod response to heavy metal insult. Metal-responsive (MR) genes and regulatory sequences are being isolated, characterized, and assayed for differential gene expression in response to heavy metal mixture pollution in the AR-CGSS. Identified promoter sequences can then be engineered into previously developed MR constructs to provide sensitive in vitro assays for environmental bioreporting of heavy metal mixtures. The results of the population genetic studies are being entered into an AR geographic information system that contains substantial biological, chemical, and geophysical information. Integrated spatial, structural, and temporal analyses of these parameters will provide invaluable information concerning environmental determinants that restrict or promote gene flow in bioreporter populations.

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Linkage Map for the Asian Tiger Mosquito [Aedes (Stegomyia) albopictus] Based on SSCP Analysis of RAPD Markers

Cited by 20 publications

References 39 publications

Quantitative trait loci determining autogeny and body size in the Asian tiger mosquito (Aedes albopictus)

Quantitative trait loci determining autogeny and body size in the Asian tiger mosquito (Aedes albopictus)

Limited Diversity of Anopheles Darlingi in the Peruvian Amazon Region of Iquitos

Molecular and genetic ecotoxicologic approaches to aquatic environmental bioreporting.

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