Linkage Relationships among Genes on Wheat Chromosome 5A That Condition Resistance to Hessian Fly

Abstract

Hessian fly [Mayetiola destructor (Say)] is a serious pest of wheat (Triticum ssp.) in many wheat growing areas of the world. A number of genes (H1‐H26) that confer resistance to Hessian fly have been described. The linkage relationships of many of these genes, which are useful in breeding wheat for resistance to Hessian fly, are unknown. Testcross analyses were made in tetraploid durum wheat(Triticum turgidum L.) and tested to biotype D of Hessian fly to determine the linkage relationships among genes H9, H10, H12, H14, H15, H16 and HI7, all conferring resistance to biotype D. Also, monosomic 5A plants of susceptible cultivar Chinese Spring were crossed as seed parents to Purdue wheat (T. aestivum L. em Thell.) lines carrying gene H10 or H12. Testcross analyses indicated that six of these genes appear to occupy a single linkage block on wheat chromosome 5A in the order H9 to H15, HIO, H17, H16, and H12. Gene H14 did not appear to be within the linkage block H9 to H12. Additionally F2 plants derived from 41‐chromosome F1 plants and F1 families derived from F2 resistant plants were tested against biotype D. F2 segregation deviated significantly from 3 resistant : 1 susceptible and F3 families from resistant F2 plants deviated significantly from 1 resistant: 2 segregating for both crosses involving H10 and H12. Thus, monosomic analyses provided additional evidence that genes H10 and H12 are likely on chromosome 5A. Linkage between these genes would not seriously limit efforts to pyramid them in breeding for resistance to the Hessian fly because the linkage values are more than 20 centimorgans.

Identification of a novel gene, H34, in wheat using recombinant inbred lines and single nucleotide polymorphism markers

“…Subsequently, other studies showed that H3, H9, and H10 were all linked to H6 (Carlson et al 1978;Stebbins et al 1982;Ohm et al 1995). Further researches revealed that H3, H5, H6, H9, H10, H11, H12, H14, H15, H16, H17, H19, H28, H29 and Hdic were all in the distal gene-rich region of wheat chromosome 1AS (Liu et al 2005, Kong et al 2005& 2008, and formed an HFR-gene cluster (about 1 cM) close to markers Xbarc263 and Xcfa2153 (Liu et al 2005).…”

“…Therefore, pyramiding several HFR genes against different biotypes may extend the life span of resistant cultivars. Molecular markers closely linked to these genes are essential for such gene pyramiding, however, many earlier reported genes were located to chromosomes using monosomic analysis (Gallun and Patterson 1977;Ohm et al 1995). Some were mapped using DNA markers, but the mapping populations used were mainly F 2 generations (Williams et al 2003;Liu et al 2005a;Liu et al 2005c;Wang et al 2006;Kong et al 2008;Yu et al 2009;Miranda et al 2010).…”

Genetics of resistance to Hessian fly (Mayetiola destructor) [Diptera : Cecidomyiida] biotype L in diploid wheat

“…Of the 27 gènes that so far hâve been identified, 20 hâve been assigned to chromosomes in hexaploid and tetraploid wheats by monosomîc and/or linkage studies. Of thèse 20, 12 (H3, H5, H6, H9, H10, H11, H12, H15, H16, H17, H25 and H27) hâve been found to be on Agenome chromosomes Ohm et al 1995Ohm et al , 1997Roberts and Gallun 1984;Stebbins étal. 1980Stebbins étal.…”

Development of STS markers linked to Hessian fly resistance gene H6 in wheat

“…Resistance in F 3 families to Hessian fly biotype B was evaluated as described by Ohm et al (1995). F 3 seedlings (20 per F 2·3 family) and all cultivars used for this study were germinated in wooden flats.…”