Linking aggregation and interfacial properties in monoclonal antibody-surfactant formulations

Kannan, Aadithya; Shieh, Ian C.; Fuller, Gerald G.

doi:10.1016/j.jcis.2019.04.060

Cited by 73 publications

(80 citation statements)

References 55 publications

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“…These results were confirmed also with a different surface-active polymer, that is, polyethylene glycol (PEG). PEG is known to be surface active and can form a monolayer at an air/water interface as well as a self-assembled film from an aqueous solution (26)(27)(28)(29)(30). Experiments of bubble bursting were performed using solutions of PEG and BSA + PEG.…”

Section: Significancementioning

confidence: 99%

Flowering in bursting bubbles with viscoelastic interfaces

Tammaro

Suja

Kannan

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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The lifetime of bubbles, from formation to rupture, attracts attention because bubbles are often present in natural and industrial processes, and their geometry, drainage, coarsening, and rupture strongly affect those operations. Bubble rupture happens rapidly, and it may generate a cascade of small droplets or bubbles. Once a hole is nucleated within a bubble, it opens up with a variety of shapes and velocities depending on the liquid properties. A range of bubble rupture modes are reported in literature in which the reduction of a surface energy drives the rupture against inertial and viscous forces. The role of surface viscoelasticity of the liquid film in this colorful scenario is, however, still unknown. We found that the presence of interfacial viscoelasticity has a profound effect in the bubble bursting dynamics. Indeed, we observed different bubble bursting mechanisms upon the transition from viscous-controlled to surface viscoelasticity-controlled rupture. When this transition occurs, a bursting bubble resembling the blooming of a flower is observed. A simple modeling argument is proposed, leading to the prediction of the characteristic length scales and the number and shape of the bubble flower petals, thus paving the way for the control of liquid formulations with surface viscoelasticity as a key ingredient. These findings can have important implications in the study of bubble dynamics, with consequences for the numerous processes involving bubble rupture. Bubble flowering can indeed impact phenomena such as the spreading of nutrients in nature or the life of cells in bioreactors.

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Section: Significancementioning

confidence: 99%

Flowering in bursting bubbles with viscoelastic interfaces

Tammaro

Suja

Kannan

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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“…The composition of the liquid film determines the physical forces that affect fluid flows and thus their stability. Protein adsorption onto an water–air interface creates a viscoelastic interface that ensures axisymmetric film drainage, whereas surfactant-laden interfaces can exhibit non-axisymmetric drainage (Kannan, Shieh & Fuller 2019). The presence of mass transport across phases can also lead to a remarkable thin film dynamics.…”

Section: Introductionmentioning

confidence: 99%

Instability and symmetry breaking in binary evaporating thin films over a solid spherical dome

et al. 2021

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“…129,130 Frequently used nonionic surfactants in mAb drug formulation are polysorbate 20 and polysorbate 80, 17,83 with poloxamers being a potential alternative. 131 Indeed, polysorbate 80 seems to be one of the most protecting surfactant against mechanical stresseinduced aggregation when compared to some other nonionic and anionic surfactants at the same concentration, 130 and also seems to be less perturbing toward mAb higher structure stability when compared to polysorbate 20. 132 This protection also depends on the protein to surfactant ratio.…”

Section: Excipientsmentioning

confidence: 98%

Physicochemical Stability of Monoclonal Antibodies: A Review

Basle

Chennell

Tokhadzé

et al. 2020

Journal of Pharmaceutical Sciences

294

162

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Monoclonal antibodies (mAbs) are subject to instability issues linked to their protein nature. In this work, we review the different mechanisms that can be linked to monoclonal antibodies instability, the parameters, and conditions affecting their stability (protein structure and concentration, temperature, interfaces, light exposure, excipients and contaminants, and agitation) and the different analytical methods used for appropriate physicochemical stability studies: physical stability assays (aggregation, fragmentation, and primary, secondary, and tertiary structure analysis), chemical stability assays and quantitative assays. Finally, data from different published stability studies of mAbs formulations, either in their reconstituted form, or in diluted ready to administer solutions, was compiled. Overall, the physicochemical stability of mAbs is linked to numerous factors such as formulation, environment, and manipulations, and must be thoroughly investigated using several complementary analytical techniques, each of which allowing specific characterization information to be harvested. Several stability studies have been published, some of them showing possibilities of extended stability. However, those data should be questioned due to potential lacks in study methodology.

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Linking aggregation and interfacial properties in monoclonal antibody-surfactant formulations

Cited by 73 publications

References 55 publications

Flowering in bursting bubbles with viscoelastic interfaces

Flowering in bursting bubbles with viscoelastic interfaces

Instability and symmetry breaking in binary evaporating thin films over a solid spherical dome

Physicochemical Stability of Monoclonal Antibodies: A Review

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