Linking Oxidative Events to Inflammatory and Adaptive Gene Expression Induced by Exposure to an Organic Particulate Matter Component

Cheng, Wan Yun; Currier, Jenna M.; Bromberg, Philip A.; Silbajoris, Robert; Simmons, Steven O.; Samet, James M.

doi:10.1289/ehp.1104055

Cited by 36 publications

(61 citation statements)

References 38 publications

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“…As shown in Figure 2A, exposure of BEAS-2B cells to concentrations of 1,2-NQ as low as 3 μ M for 10 min resulted in a marked increase in the concentration of protein sulfenylation, the magnitude of which approximated that induced by exposure to 1000 μ M H 2 O 2 . In accord with our previous report, 12 exposure of BEAS-2B cells to 0–100 μ M 1,2-NQ for 10 min did not result in cytotoxicity.…”

Section: Resultssupporting

confidence: 93%

“…Our previous study implicated 1,2-NQ-induced H 2 O 2 generation in the induction of inflammatory gene expression, suggesting a mechanism of toxicity that does not require the adduction of proteins. 12 This study further supports the importance of 1,2-NQ-induced H 2 O 2 , as it was shown to induce sulfenylation of thiols on regulatory proteins. Given the critical role of protein sulfenylation in many redox-dependent physiological processes, 19,22,43 the inappropriate induction of sulfenylation by 1,2-NQ exposure represents a significant, previously unrecognized mechanism of cellular toxicity.…”

Section: Discussionsupporting

confidence: 77%

“…37 We have previously reported that exposure to environmentally relevant concentrations of 1,2-NQ results in elevation of H 2 O 2 levels in BEAS-2B cells. 12 We therefore examined the role of H 2 O 2 generation in 1,2-NQ-induced protein sulfenylation in BEAS-2B cells. In agreement with our previous studies using the H 2 O 2 sensor, HyPer, 10 μ M 1,2-NQ induced a rapid increase in the level of cytosolic H 2 O 2 (Figure S1).…”

Section: Resultsmentioning

confidence: 99%

“…We report here that exposure to 1,2-NQ at concentrations that are theoretically achievable in real world scenarios 12 induces sulfenylation of regulatory proteins in human airway epithelial cells (HAEC).…”

Section: Introductionmentioning

confidence: 99%

“…8–10 It can also undergo redox cycling reactions to form reactive oxygen species (ROS) such as hydrogen peroxide (H 2 O 2 ) that contribute to oxidative stress. 11,12 Investigations to date have predominantly focused on the effect of 1,2-NQ adduction, while the biological ramifications of 1,2-NQ-induced oxidative stress have yet to be fully investigated.…”

Section: Introductionmentioning

confidence: 99%

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Protein Sulfenylation: A Novel Readout of Environmental Oxidant Stress

Wages

Lavrich

Zhang

et al. 2015

Chem. Res. Toxicol.

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Oxidative stress is a commonly cited mechanism of toxicity of environmental agents. Ubiquitous environmental chemicals such as the diesel exhaust component 1,2-naphthoquinone (1,2-NQ) induce oxidative stress by redox cycling, which generates hydrogen peroxide (H2O2). Cysteinyl thiolate residues on regulatory proteins are subjected to oxidative modification by H2O2 in physiological contexts and are also toxicological targets of oxidant stress induced by environmental contaminants. We investigated whether exposure to environmentally relevant concentrations of 1,2-NQ can induce H2O2-dependent oxidation of cysteinyl thiols in regulatory proteins as a readout of oxidant stress in human airway epithelial cells. BEAS-2B cells were exposed to 0–1000 μM 1,2-NQ for 0–30 min, and levels of H2O2 were measured by ratiometric spectrofluorometry of HyPer. H2O2-dependent protein sulfenylation was measured using immunohistochemistry, immunoblotting, and isotopic mass spectrometry. Catalase overexpression was used to investigate the relationship between H2O2 generation and protein sulfenylation in cells exposed to 1,2-NQ. Multiple experimental approaches showed that exposure to 1,2-NQ at concentrations as low as 3 μM induces H2O2-dependent protein sulfenylation in BEAS-2B cells. Moreover, the time of onset and duration of 1,2-NQ-induced sulfenylation of the regulatory proteins GAPDH and PTP1B showed significant differences. Oxidative modification of regulatory cysteinyl thiols in human lung cells exposed to relevant concentrations of an ambient air contaminant represents a novel marker of oxidative environmental stress.

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Section: Resultssupporting

confidence: 93%

Section: Discussionsupporting

confidence: 77%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protein Sulfenylation: A Novel Readout of Environmental Oxidant Stress

Wages

Lavrich

Zhang

et al. 2015

Chem. Res. Toxicol.

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Mutagenicity and oxidative damage induced by an organic extract of the particulate emissions from a simulation of the deepwater horizon surface oil burns

DeMarini

Warren

Lavrich

et al. 2017

Environ and Mol Mutagen

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Emissions from oil fires associated with the "Deepwater Horizon" explosion and oil discharge that began on April 20, 2010 in the Gulf of Mexico were analyzed chemically to only a limited extent at the time but were shown to induce oxidative damage in vitro and in mice. To extend this work, we burned oil floating on sea water and performed extensive chemical analyses of the emissions (Gullett et al., Marine Pollut Bull, in press, ). Here, we examine the ability of a dichloromethane extract of the particulate material with an aerodynamic size ≤ 2.5 µm (PM ) from those emissions to induce oxidative damage in human lung cells in vitro and mutagenicity in 6 strains of Salmonella. The extract had a percentage of extractable organic material (EOM) of 7.0% and increased expression of the heme oxygenase (HMOX1) gene in BEAS-2B cells after exposure for 4 hr at 20 µg of EOM/ml. However, the extract did not alter mitochondrial respiration rate as measured by extracellular flux analysis. The extract was most mutagenic in TA100 +S9, indicative of a role for polycyclic aromatic hydrocarbons (PAHs), reflective of the high concentrations of PAHs in the emissions (1 g/kg of oil consumed). The extract had a mutagenicity emission factor of 1.8 ± 0.1 × 10 revertants/megajoule in TA98 +S9, which was greater than that of diesel exhaust and within an order of magnitude of open burning of wood and plastic. Thus, organics from PM of burning oil can induce oxidative responses in human airway epithelial cells and are highly mutagenic. Environ. Mol. Mutagen. 58:162-171, 2017. © 2017 Wiley Periodicals, Inc.

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Effect of temperature and aging on the adsorption and desorption of menadione in soil

Gao

Gong

et al. 2017

Env Prog and Sustain Energy

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In this study, the effect of soil temperature and aging on the adsorption and desorption of menadione was investigated using the batch equilibrium method. The obtained isotherm data can be well fitted to the linearized form of the Freundlich equation, with R2 values ranging between 0.91 and 1.00. The adsorption and desorption of menadione in non‐aged (control) soil increased significantly when the temperature ranged between 15 and 37°C. Among soils that were amended with menadione and aged for 72 h, the sample with 2 μg·g−1 amendment showed significantly higher adsorption/desorption than those with 10 and 20 μg·g−1 amendments (P < 0.05). Thermodynamic and isotherm hysteresis analyses demonstrated that the sorption of menadione on the control and aged soils was a spontaneous and endothermic process. Physical sorption was the predominant mechanism, and there was negative hysteresis during desorption. Furthermore, thermodynamic calculation and infrared spectral analyses suggested that the main sorption force was hydrogen bonding in all the soil samples, and the entropy gain in the whole adsorbate‐adsorbent system was the main thermodynamic driving force. © 2017 American Institute of Chemical Engineers Environ Prog, 36: 997–1004, 2017

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Linking Oxidative Events to Inflammatory and Adaptive Gene Expression Induced by Exposure to an Organic Particulate Matter Component

Cited by 36 publications

References 38 publications

Protein Sulfenylation: A Novel Readout of Environmental Oxidant Stress

Protein Sulfenylation: A Novel Readout of Environmental Oxidant Stress

Mutagenicity and oxidative damage induced by an organic extract of the particulate emissions from a simulation of the deepwater horizon surface oil burns

Effect of temperature and aging on the adsorption and desorption of menadione in soil

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