Linking Telomere Regulation to Stem Cell Pluripotency

Liu, Lin

doi:10.1016/j.tig.2016.10.007

Cited by 54 publications

(58 citation statements)

References 206 publications

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“…Telomere elongation with increasing passage depends on the mechanisms of both telomerase and recombination‐based alternative lengthening of telomeres . When crotonylation induced by crotonic acid, Zscan4 was found activated, and T‐SCE level increased, which maintained telomeres and reduced telomere damage during chemical induction .…”

Section: Functional Roles Of Lysine Crotonylationmentioning

confidence: 99%

Functions and mechanisms of lysine crotonylation

Wan

Chu

et al. 2019

J Cellular Molecular Medi

104

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Lysine crotonylation is a newly discovered post‐translational modification, which is structurally and functionally different from the widely studied lysine acetylation. Recent advances in the identification and quantification of lysine crotonylation by mass spectrometry have revealed that non‐histone proteins are frequently crotonylated, implicating it in many biological processes through the regulation of chromatin remodelling, metabolism, cell cycle and cellular organization. In this review, we summarize the writers, erasers and readers of lysine crotonylation, and their physiological functions, including gene transcription, acute kidney injury, spermatogenesis, depression, telomere maintenance, HIV latency and cancer process. These findings not only point to the new functions for lysine crotonylation, but also highlight the mechanisms by which crotonylation regulates various cellular processes.

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Section: Functional Roles Of Lysine Crotonylationmentioning

confidence: 99%

Functions and mechanisms of lysine crotonylation

Wan

Chu

et al. 2019

J Cellular Molecular Medi

104

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“…The membrane was washed twice in 0.2 N Na phosphate, 2% SDS, and 1 mM EDTA at room temperature, once in 0.1 N Na phosphate, 2% SDS, and 1 mM EDTA at 50°C, and analyzed by by using Chemiluminescent Nucleic Acid Detection Module kit (Thermo Fisher Scientific). Biotin-TERRA probe (59-CCCTAA-39) 6 and biotin-18s rRNA probe (59-CCATCCAATCGGTAGTAGCG-39) were used.…”

Section: Northern Blotmentioning

confidence: 99%

“…Secondary Abs were obtained from Cell Signaling Technology. TelC-FITC probe was obtained from Dako (Copenhagen, Denmark) and the biotin-labeled (59-CCCTAA-39) 6 probe was from Takara (Kyoto, Japan).…”

Section: Absmentioning

confidence: 99%

“…Pluripotent stem cells (PSCs), including human embryonic stem cells (hESCs), mouse embryonic stem cells (mESCs), somatic cell nuclear transfer ESCs, and induced pluripotent stem cells (iPSCs), exhibit their capacity for 3-germ-layer differentiation and self-renewal via unlimited symmetric cell division in vitro (5). Maintenance of telomere length is critical for the unlimited self-renewal, pluripotency, and chromosomal stability of PSCs (6)(7)(8).…”

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confidence: 99%

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Role of telomeric repeat–containing RNA in telomeric chromatin remodeling during the early expansion of human embryonic stem cells

et al. 2017

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This study aimed to explore the role of telomeric repeat-containing RNA (TERRA) in telomeric chromatin remodeling during the early expansion of human embryonic stem cells (hESCs). During the derivation of hESCs, histone demethylation in the telomeric region facilitates telomerase-mediated telomere elongation. An adequate telomere repeat is essential for hESCs to acquire and/or maintain the unlimited symmetric division, which suggests that there is a link between pluripotency and telomere maintenance. The present study found that the gradual decrease in TERRA levels and related TERRA foci were correlated with telomeric length elongation in the early expansion of hESCs. In addition, TERRA participated in telomeric chromatin remodeling by cooperating with SUV39H1 (suppressor of variegation 3-9 homolog 1/2) to propagate telomeric heterochromatin marker, histone H3 trimethylation of lysine 9. Moreover, the fibroblast growth factor signaling pathway, which is activated in hESCs, could suppress TERRA levels telomeric repeat factor 1, which results in reduced SUV39H1 recruitment by TERRA at the telomere. Taken together, these results highlight the role of TERRA in hESC telomere elongation and homeostasis in the acquisition and/or maintenance of stem cell pluripotency.-Zeng, S., Liu, L., Sun, Y., Lu, G., Lin, G. Role of telomeric repeat-containing RNA in telomeric chromatin remodeling during the early expansion of human embryonic stem cells.

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“…H3K9me3 levels negatively regulate telomere lengths, but the histone acetylation levels positively regulate telomere lengths (Liu, 2017). H3ac and H3K9ac protein levels did not differ between Hdac6 overexpression and WT ES cells, whereas H3K9me3 levels were reduced in Hdac6 overexpression ES cells compared with WT ES cells (Fig.…”

mentioning

confidence: 99%