Lipase biosensor for tributyrin and pesticide detection

Recent advances in biosensors based on enzyme inhibition

“…Moreover, after each single experiment, the enzyme looses a part of its initial activity, and thus the lifetime of such sensors is limited to only few cycles of reuse. The addition of a particular reagent can regenerate the enzyme activity (Du et al, 2007 b;Liu et al, 2006), but extends its lifetime for only few cycles, since the original activity of the enzyme can never be restored at 100% (Kartal et al, 2007). Therefore, a single experiment approach, like the use of screen-printed electrodes , Arduini et al 2013, Arduini et al, 2007 is justified and highly desired in the case of irreversible inhibition.…”

“…Indeed, inhibition of lipase by methyl-parathion requires 15 minutes (Kartal et al, 2007), inhibition of invertase by mercury and silver needs 10-20 minutes and 10 minutes incubation time was sufficient to inhibit glucose oxidase by atrazine (Qing Yang et al, 2010).…”

LIPASE ACTIVITIY OF MIXTURE OF FERMENTED AVOCADO (Persea americana), BANANA (Moses paradisiaca) AND SNAKEFRUIT (Salacca zalacca)

“…The biocatalytic properties of this lipase allow its use for various purposes such as detergent formulation (Thirunavukarasu et al, 2008), biosensors (Kartal et al, 2007), food industry (Ferrer et al, 2005), dairy industry and oleo industry Chemistry (Sana et al, 2004) ester synthesis (Jin et al, 2013), pharmaceutical and waste treatment (Savendsen, 2000). Lipase is also widely used in the processing of fats or oils, chemical synthesis, paper synthesis, cosmetic production and also biodiesel industry (Sari, 2012).…”

Genome mining of fungal lipid-degrading enzymes for industrial applications

“…Therefore, they are commonly used in processing in biotechnological industries, including food (De Maria et al, 2007;Rajan et al, 2008;Keng et al, 2009), cosmetics and personal care products, oleochemicals (Xia et al, 1996;Lai et al, 2005;Saisubramanian et al, 2006;Goswami et al, 2013) and biomedical industries as well as bioremediation (Lanciotti et al, 2005;Cammarota & Freire, 2006) and biosensor applications (Albertsson & Varma, 2003;Kartal et al, 2007;Sandoval et al 2010). Nowadays, yeast and fungi are very attractive lipaseproducing systems for industrial applications due to the versatility of their properties and ease of mass production (Fickers et al, 2011;Singh & Mukhopadhyay, 2012).…”