Lipase-Entrapped Colloidosomes with Tunable Positioning at the Oil–Water Interface for Pickering Emulsion-Enhanced Biocatalysis

Abstract

Pickering interfacial biocatalysis (PIB) paves the way for efficient enzymatic catalysis in the biphasic system. However, the Pickering interfacial biocatalysts located on the oil–water interface still face the inevitable deactivation when one of the phases contains the reactant that inactivates the enzyme. Herein, the positioning of lipase-entrapped colloidosomes (LECs) at the emulsion interface is rationally designed by physically tuning the wettability, which allows LECs to protrude into the selected phase to protect the lipase away from the damage of the reactant. As a proof of concept, LECs with different positioning at the interface are used as Pickering interfacial biocatalysts to produce biodiesel by esterification of lauric acid and methanol. Impressively, the LECs that protrude into the oil phase possess an optimal catalytic performance to protect more lipases away from the damage of the reactant of short-chain alcohol, which shows an 8.18-fold enhancement in specific activity relative to the free lipase, reach a biodiesel yield of 80.37% after 8 h, and retain the 96.44% of relative activity after 10 cycles. This study provides a novel and robust platform for Pickering emulsion-enhanced biocatalysis.

Ultraviolet/Visible Light-Responsive Pickering Interfacial Biocatalysis: Efficient and Robust Platform for Bioconversions

“…Take 5 μL of the emulsion on a glass slide, irradiate the emulsion with a UV lamp and observe the demulsification by CLSM. The total surface area ( S ) of the emulsion (cm 2 ) was determined to formula S = v l × w × d × n × 4 π r 2 = 2 π italicnvr lw Where v is the volume of the emulsion (cm 3 ), l is the length of the emulsion droplet counting area (cm), w is the width of the emulsion droplet counting area (cm), r is the average radius of emulsion droplets (cm), and n is the number of emulsion droplets.…”

“…Take 5 μL of the emulsion on a glass slide, irradiate the emulsion with a UV lamp and observe the demulsification by CLSM. The total surface area (S) of the emulsion (cm 2 ) was determined to formula 1 35…”

Surfactants Influencing the Biocatalytic Performance of Natural Alkane-Degrading Bacteria via Interfacial Biocatalysis in Pickering Emulsions

“…The oil–water interfaces of emulsions provide a new strategy for heterogeneous chemical reactions where both water-soluble chemicals in the water phase and water-insoluble chemicals in the oil phase react smoothly catalyzed by catalyst at the water–oil interfaces, such as lipid hydrolysis catalyzed by lipase. − This is known as interfacial catalysis. When particles act as both Pickering emulsifiers and catalysts, Pickering emulsions provide a robust platform for heterogeneous chemical reactions. , The diversity of catalysts ranges from inorganic/organic catalyst to immobilized enzymes , and even to immobilized microbial cells . In our previous studies, the hydrophobic bacteria ( Mycobacterium sp.…”

“…When particles act as both Pickering emulsifiers and catalysts, Pickering emulsions provide a robust platform for heterogeneous chemical reactions. 15,16 The diversity of catalysts ranges from inorganic/organic catalyst 17 to immobilized enzymes 18,19 and even to immobilized microbial cells. 20 In our previous studies, the hydrophobic bacteria (Mycobacterium sp.…”

Efficient construction of multi‐stimuli responsive colloidosomes for intelligent transmembrane transport

“…[28][29][30] Furthermore, by modifying the NPs, the functionalization of colloidosomes can be easily achieved. [31][32][33] By controlling the size of NPs and crosslink degree, the pore diameter and permeability of shells can be regulated. [34][35][36] However, the preparation of colloidosomes with real-time adjustable permeability for regulating the activity of encapsulated microorganisms is still challenging.…”