Lipase-Producing Bacterium and its Enzyme Characterization

Abstract

Recently, renewable energy is very important use for industrial development, transport because it is environmental friendly and can reduce high cost of imported fossil fuel. The evolution of biodiesel production has been reported in many researchers. Transesterification process which is mainly reaction for biodiesel is required catalysts such as acid, base catalyst or biocatalyst. In this study, 134 isolates were selected from soil contaminated with oil in Khon Kaen region by using culture medium containing 1% olive oil and 0.0001% rhodamine B with incubation at 30°C for 48 h. These lipase-producing bacteria were also determined their activities on agar medium with 1% tributyrin. The isolate NA37 showed high lipase activity (190 mU/ml) detecting with p-nitrophenyl palmitate as a substrate. Cooking-palm oil was contributed lipase production of the NA37 more than other oils. The optimum temperature and pH for lipase production is 30°C and pH 9, resulting in lipase activity of 481 mU/ml. 

Determination the Lipase Activity of Staphylococcus sp. Strain Isolated from Clinical Specimens

“…The colonies of lipase producing bacteria were showed halos of orange fluorescent during 48hrs of incubation at 37°C, This appearance may be due to the hydrolysis of substrate, or due to the construction of conjugate of a Rhodamine -B long chain fatty acid. The results in the present study agree with study of 21,13,[22][23][24][25][26] . Qualitative screening of Pseudomonas sp LSK25 lipase showed positive evidence of lipase production.…”

Enhanced production of thermostable lipase from Bacillus cereus ASSCRC-P1 in waste frying oil based medium using statistical experimental design

“…These results were more than that obtained from several researchers as Guzman and Virginia (2008) who detected lypolytic activity of 0.079 U/ml by Bacillus pumilus LV01 isolated from water samples and maximum lipase production of 12 U/ml by Pseudomonas gessardii selected from a soil sample (Veerapagu et al, 2013). These results were in accordance to Boonmahome and Mongkolthanaruk (2013) who found that Pseudomonas aeruginosa isolate NA37 selected from soil contaminated with oil in Khon Kaen region showed high lipase activity (190 mU/ml) detecting with p-nitrophenyl palmitate as a substrate. On the other hand, Dhiman and Chapadgaonkar (2013) isolated and identified as Bacillus ISC 1, from a site of regular oil spill, showed high lipase production of 25 U/ml.…”

Detection of extracellular enzymatic activity in microorganisms isolated from waste vegetable oil contaminated soil using plate methodologies

“…The results were reviewed after 48 h of incubation, time reported to allow better contact between substrate and strains (Feng et al, 2010;Boonmahome, 2013) and shown in Table 3, in which strain 12 presented the highest enzymatic activity with a halo diameter of 10.7 mm followed by strain 38 with 10.6 mm, both considered as good producers according to the criteria established by Hou and Johnston (1992). From the results of the statistical analysis (p<0.05), strains 12, 38, 35, 30 and 31 were selected for further study and given that from this analysis the selected strains were part of the same group, from spectrophotometric quantification, we were able to discern and stablish significant difference between them (data not shown).…”