Lipase purification by various techniques and its thermostabilization in presence of surface active additives

Abstract

: Crude porcine pancreatic lipase was puriÐed by removing waterinsoluble impurities (residual lipid material) associated with it by a conventional method, using hydrotropic additives and by liquid coacervate extraction. Maximum yield with good recovery of activity was obtained when hydrotropes were used to separate the associated lipids from lipase. The thermostability of the enzyme was also checked in the solutions of additives such as sodium butyl monoglycol sulfate (Na-BMGS), proline and Triton X-114. In Na-BMGS solutions above a concentration of 0É2 mol dm~3 the lipase activity decreased beyond 50¡C whereas in 1 mol dm~3 proline solution it was retained even at 80¡C, showing a good thermostabilizing e †ect. However, in the presence of Triton X-114 the enzyme was completely inactivated with increase in temperature.1998 SCI. ( J. Chem. T echnol. Biotechnol. 71, 155È161 (1998)

Estudo do comportamento de sistemas hidrotrópicos

“…As características peculiares dos hidrótropos refletem-se na gama de aplicações hoje encontradas para estas moléculas, tais como produtos de limpeza e laVanderia [9], farmacêutica [14,15,16,17,18], alimentação [19,20], e ciência dos cosméticos [21,22]. As soluções hidrotrópicas tendem a separar o soluto com a diluição, o que possibilita aplicações diversas como agente de extração e separação de substâncias [8].…”