Lipid accumulation by a cellulolytic strain ofAspergillus niger

Abstract

Lipid accumulation by a cellulolytic mold, Aspergillus niger, was studied. The amount of lipid accumulated ranged from 13.6-16.6% on various carbon sources, namely glucose, xylose, avicel (microcrystalline cellulose) and bagasse (a natural lignocellulosic substrate). Neutral lipids, phospholipids and glycolipids of the mycelia varied from 41.0-46.2%, 34.9-38.4% and 18.7-22.6% of total lipids, respectively. Unsaturated fatty acids comprised around 80% of total fatty materials with linoleic and oleic acid predominating. Of the four nitrogen sources tested, NH4Cl was the best source for lipid synthesis from cellulose (bagasse). Optimum temperature range for growth and lipid synthesis was 25-30 degrees C.

Production of Aspergillus niger biomass on sugarcane distillery wastewater: physiological aspects and potential for biodiesel production

“…Comparatively, Zheng et al [ 47 ] showed that A. niger grown on glucose or xylose as sole carbon source led to a production of 5.8 and 4.6 g L −1 of biomass with a lipid content of 9.6 and 8% respectively. Similarly, A. niger grown on bagasse led to a fungal biomass of about 1.9 g L −1 with a lipid content of 13.6% [ 48 ]. Also, André et al [ 49 ] showed that two different A. niger strains cultivated in crude glycerol could produce up to 8.2 g L −1 of biomass with a lipid content of about 50% (meaning about 3 g L −1 of lipids).…”

“…Stearic (18:0) and α-linolenic (18:3, n-3) acids were produced to a lesser extent by A. niger on both media (Table 4 ). Singh [ 48 ] reported that A. niger biomass grown on glucose medium contained mostly linoleic acid (50%) and to a lesser extent, palmitic, stearic and linolenic acids (8.3, 5.2 and 6% respectively). Whatever the medium used, linolenic acid appeared as the major intracellular lipid of A. niger biomass; however, it can be noticed that A. niger grown on glucose medium and on LAM were richer in oleic acid than biomass grown on SDW (23.5 and 28.19 against 17.23%) [ 48 ].…”

“…Singh [ 48 ] reported that A. niger biomass grown on glucose medium contained mostly linoleic acid (50%) and to a lesser extent, palmitic, stearic and linolenic acids (8.3, 5.2 and 6% respectively). Whatever the medium used, linolenic acid appeared as the major intracellular lipid of A. niger biomass; however, it can be noticed that A. niger grown on glucose medium and on LAM were richer in oleic acid than biomass grown on SDW (23.5 and 28.19 against 17.23%) [ 48 ]. By comparison, biodiesel from Yarrowia lipolytica [ 50 ] contained twice higher oleate esters but less than three times linoleate esters than biodiesel from A. niger grown on SDW.…”

Impact on Wastewater Quality of Biopellets Composed of <i>Chlorella vulgaris</i> and <i>Aspergillus niger</i> and Lipid Content in the Harvested Biomass

“…Lipid accumulation, in both microalgae and fungi, is dependent on the growing conditions, with stress conditions being favourable for lipid accumulation by microalgae [32] and with the quantity and quality of carbon and nitrogen sources suggested to be important factors for A. niger [33]. The lipid content of the microalgal biomass harvested on day 5 was 5.9% of dry biomass and that of the algal-fungal biomass harvested on day 8 was 3.4% of dry biomass (Table 2).…”