Lipid and fatty acid composition of muscle, liver, ovary, and peritoneal fat in wild flathead grey mullet (Mugil cephalus) according to ovarian development

Ramos-Júdez, Sandra; Estévez, Alicia; González-López, Wendy Ángela; Duncan, Neil

doi:10.1016/j.theriogenology.2022.12.046

Cited by 8 publications

(7 citation statements)

References 34 publications

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“…Previously, it has been shown that nanoparticles with a hydrodynamic size of less than 200 nm are capable of membrane penetration, although this can be influenced by shape and/or surface functional groups of the nanoparticles, , as verified in various organisms at the cellular and individual levels. , Indeed, size-selective bioaccumulation of nanoparticles (<100 nm) in the reproductive organs of the mouse is reportedly associated with increased blood-vessel density during ovulation due to the blood-vessel membrane permeability of nanoplastics . Moreover, the fact that lipids constitute a major proportion of fish egg yolk (in the form of lipid droplets) supports the bioaccumulation of hydrophobic nanoplastics. − Given that Oryzias spp. has a daily ovulation cycle, and considering the high blood-vessel density during ovulation, a synergistic uptake mechanism may be responsible for the strong nanoplastic bioaccumulation in medaka ovaries.…”

Section: Resultsmentioning

confidence: 99%

Insights into Tissue-Specific Bioaccumulation of Nanoplastics in Marine Medaka as Revealed by a Stable Carbon Isotopic Approach

Yeo,

Shim,

Kim

et al. 2023

Environ. Sci. Technol. Lett.

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Despite the high bioavailability and potentially extensive presence of nanoplastics in aquatic environments, the biological fate of nanoplastics is largely unknown because of analytical limitations in detection and quantification. Fluorescently labeled nanoplastics are widely used to detect bioaccumulation, but this method is prone to false-positive results due to the leaching of fluorescent dyes. Here we propose a novel stable carbon isotopic approach to detect and quantify nano- and microplastics in a complex organic matrix. Because carbon is the major component of plastics (>87% in polystyrene), it is possible to investigate tissue-specific bioaccumulation of nano- and microplastics in the medaka Oryzias melastigma by quantifying the contribution of plastic particles as an end-member in the composition of stable carbon isotopes in different tissues. In addition to the digestive organs (e.g., the gut and intestines) that are constantly exposed to the water column via ingestion, nanoplastics were shown to selectively bioaccumulate in the gills and ovary, implying a unique mode of action of bioaccumulation based on the physicochemical properties of the nanoparticles. These findings should improve our understanding of the tissue-specific bioaccumulation of nano- and microplastics in aquatic organisms.

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Section: Resultsmentioning

confidence: 99%

Insights into Tissue-Specific Bioaccumulation of Nanoplastics in Marine Medaka as Revealed by a Stable Carbon Isotopic Approach

Yeo,

Shim,

Kim

et al. 2023

Environ. Sci. Technol. Lett.

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“…The gonadal development of prepubertal meagres was established by evaluating the presence of germ cells in six random optical areas at 20x magnification, although a maximum gonadal development was also established by evaluating the entire gonad due the low presence of sex specific germ cell stages of development. Female germs cells were classified as: chromatin nucleolar stage of primary growth (PGcn) characterized by small oocytes with a large single nucleolus surrounded by a thin layer of citoplasm; perinuclear stage of primary growth (PGps) with a bigger oocyte due to the enlargement of the nucleus and the appearance of multiple nucleoli (West, 1990; Ramos-Júdez et al, 2023). Male germs cells were classified as: type A undifferentiated spermatogonia (StgAund) characterized by being the largest male germ cells and having a large nucleus in addition to one or two nucleoli; type A differentiated spermatogonia (StgAdiff), smaller than StgAund and present in cysts in groups of 2 to 8 germs cells linked by cytoplasmic bridges; type B spermatogonia (StgB) with a smaller size than the previous cells and present in cysts in groups of 16 or more germs cells; spermatocyte (spc) characterized by an increase in cell and nucleus size with respect to StgB due to entry into meiosis (condensing chromosomes), moreover an increase in germs cells per cyst; spermatid (spd) with a significant reduction in cell size and increase of germs cells per cyst; and spermatozoa (spz) with the presence of a flagellum and reduction in cell size (Leal et al, 2009; Schulz et al, 2010; Lacerda et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

In vivoeffect of recombinant FSH and LH administered to meagre (Argyrosomus regius) at the initial stages of sex differentiation

González Cid,

Giménez,

Duncan

2023

Preprint

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Recombinant gonadotropins, follicle stimulating (rFSH) and luteinizing hormone (rLH), offer the potential to induce gametogenesis in prepubertal fish. This study aimed to determine thein vivoeffect ofArgyrosomus regiusrFSH (arrFSH) andArgyrosomus regiusrLH (arrLH) administered to prepubertal meagre juveniles at the initial stages of sex differentiation. Meagre single-chain recombinant gonadotropins, arrFSH and arrLH were produced with the CHO expression system by Rara Avis Biotec, S. L. Juvenile meagre, 9-months old with mean weight of 222 ± 36 g (mean ± SD) were randomly distributed into seven experimental groups (n = 8 per group) that were treated weekly for three weeks with an acute injection of 6, 12 or 18 μg kg-1of arrFSH (groups, 6-arrFSH, 12-arrFSH and 18-arrFSH) or 6, 12 or 18 μg kgkg-1of arrLH (groups, 6-arrLH, 12-arrLH and 18-arrLH) or saline solution (Control group). Two more groups (n=8) were set up and treated for 6 weeks, with 12 μg kg-1of arrFSH or saline Control. The fish were held in a 10 m3tank with natural photoperiod (Feb. - March) and temperature 16.1 ± 0.4°C. At the start of the experiment (n = 8) and end of the three week experiment all fish were sacrificed and gonads dissected. Gonads were weighed, fixed in Bouins solution and processed for histological analysis. Blood was sampled from all fish at the start and end of the experiment (week 3 and 6) for 17β-estradiol (E2) and 11-ketotestosterone (11-KT) analysis. Juvenile meagre at the start of the experiment were in the initial stages of sexual differentiation, indicated by the presence of the ovarian or testes lumen that was surrounded by undifferentiated embryonic germ stem cells and somatic cells. At the end of the experiment, there was no significant difference in gonadosomatic index (GSI) amongst control (initial and saline treated) and the experimental groups. After three weeks of application of arrFSH, arrLH or saline all fish presented a similar gonadal structure as at the start of the experiment. However, the incidence of isolated developing germ cells (principally spermatogonia, spermatocytes, spermatids, but also perinucleolar stage oocytes) generally increased in arrGTH treated meagre. A mean of 44 % of meagre treated with arrFSH or arrLH presented isolate developing germ cells, mainly male cells. Plasma steroid levels of E2 decreased significantly from the start of the experiments to the end. At the end of the experiment there were no differences in plasma E2 amongst Control fish and rGTH treated fish. Plasma 11-KT showed no change from the start of the experiment to week 3. However, a significant increase was observed in the arrFSH group after six weeks of treatment compared to the start of the experiment and the control group on week 6. The application of arrFSH or arrLH to meagre at the initial stages of sex differentiation did not stimulate steroid production until week six and had a limited, but evident effect on the development of isolated germ cells. The rGTHs, arrFSH or arrLH did not stimulate large developmental changes in undifferentiated gonads.

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“…Fatty acid profile was obtained as described by Ramos-Júdez et al. ( 29 ). In brief, transmethylated fatty acids from total lipids were extracted, purified, and finally quantified by gas-liquid chromatography on a Thermo Trace GC (Thermo Fisher, Spain) coupled to a TRACE™ TR-FAME GC Column (Thermo Scientific, Spain), using heneicosylic acid (21:0) as internal standard (ref.…”

Section: Methodsmentioning

confidence: 99%

“…The protocols described by Lowry et al (25), Folch et al (26), Dubois et al (27), and AOAC (28) were followed to determine the levels of total protein, lipids, carbohydrates, and ash content in livers and fillets. Fatty acid profile was obtained as described by Ramos-Judez et al (29). In brief, transmethylated fatty acids from total lipids were extracted, purified, and finally quantified by gas-liquid chromatography on a Thermo Trace GC (Thermo Fisher, Spain) coupled to a TRACE ™ TR-FAME GC Column (Thermo Scientific, Spain), using heneicosylic acid (21:0) as internal standard (ref.…”

Section: Proximate and Fatty Acid Compositionmentioning

confidence: 99%

The potential of a combination of pungent spices as a novel supplement in gilthead seabream (Sparus aurata) diets to aid in the strategic use of fish oil in aquafeeds: a holistic perspective

Ruiz,

Sanahuja,

Andree

et al. 2023

Front. Immunol.

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This work studied the potential of a combination of pungent spices (capsicum, black pepper, ginger, and cinnamaldehyde) to be used as a supplement in diets of gilthead seabream (Sparus aurata; 44.1 ± 4.2 g). During 90 days, fish were fed three experimental diets with low inclusion of fish oil and containing poultry fat as the main source of lipids, supplemented with graded levels of the tested supplement: 0 (control), 0.1 (SPICY0.1%), and 0.15% (SPICY0.15%). As a result, the pungent spices enhanced the growth performance, the activity of the bile-salt-activated lipase in the intestine, and decreased fat deposit levels within enterocytes. The SPICY0.1% diet reduced the feed conversion ratio and the perivisceral fat index and lipid deposits in the liver. Moreover, the ratio of docosahexaenoic acid/eicosapentaenoic acid in fillet increased in fish fed the SPICY0.1% diet, while the hepatic levels of docosahexaenoic acid and total n-3 polyunsaturated fatty acids increased in fish fed the SPICY0.15% diet. Furthermore, there was an effect on the expression of some biomarkers related to lipid metabolism in 2-h postprandial fish (fasn, elovl6, scd1b, cyp7a1, lpl, and pparβ), and in 48 h fasted-fish fed with the SPICY0.1% diet, a regulation of the intestinal immune response was indicated. However, no significant differences were found in lipid apparent digestibility and proximate macronutrient composition. The spices did not affect biomarkers of hepatic or oxidative stress. No differences in microbial diversity were found, except for an increase in Simpson’s Index in the posterior intestine of fish fed the SPICY0.1% diet, reflected in the increased relative abundance of the phylum Chloroflexi and lower relative abundances of the genera Campylobacter, Corynebacterium, and Peptoniphilus. In conclusion, the supplementation of gilthead seabream diets with pungent spices at an inclusion of 0.1% was beneficial to enhance growth performance and feed utilization; reduce fat accumulation in the visceral cavity, liver, and intestine; and improve the fish health status and condition. Results suggest that the tested supplement can be used as part of a nutritional strategy to promote a more judicious use of fish oil in fish diets due to its decreasing availability and rising costs.

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Lipid and fatty acid composition of muscle, liver, ovary, and peritoneal fat in wild flathead grey mullet (Mugil cephalus) according to ovarian development

Cited by 8 publications

References 34 publications

Insights into Tissue-Specific Bioaccumulation of Nanoplastics in Marine Medaka as Revealed by a Stable Carbon Isotopic Approach

Insights into Tissue-Specific Bioaccumulation of Nanoplastics in Marine Medaka as Revealed by a Stable Carbon Isotopic Approach

In vivoeffect of recombinant FSH and LH administered to meagre (Argyrosomus regius) at the initial stages of sex differentiation

The potential of a combination of pungent spices as a novel supplement in gilthead seabream (Sparus aurata) diets to aid in the strategic use of fish oil in aquafeeds: a holistic perspective

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