Lipid bilayers: Phase behavior and nanomechanics

Redondo-Morata, Lorena; Losada-Pérez, Patricia; Giannotti, Marina I.

doi:10.1016/bs.ctm.2020.08.005

Cited by 20 publications

(17 citation statements)

References 184 publications

(199 reference statements)

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“…SLB is an artificial lipid bilayer system formed at solid-liquid interfaces. It is a useful biomembrane model system to investigate activities of membrane proteins in and on lipid bilayer [ 20 , 21 , 22 , 23 , 24 , 25 ]. Recently, there have been studies on the construction of SLB systems containing specific lipid domains to investigate the function of peptides acting specifically on the domains [ 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Physical Properties and Reactivity of Microdomains in Phosphatidylinositol-Containing Supported Lipid Bilayer

et al. 2021

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We characterized the size, distribution, and fluidity of microdomains in a lipid bilayer containing phosphatidylinositol (PI) and revealed their roles during the two-dimensional assembly of a membrane deformation protein (FBP17). The morphology of the supported lipid bilayer (SLB) consisting of PI and phosphatidylcholine (PC) on a mica substrate was observed with atomic force microscope (AFM). Single particle tracking (SPT) was performed for the PI+PC-SLB on the mica substrate by using the diagonal illumination setup. The AFM topography showed that PI-derived submicron domains existed in the PI+PC-SLB. The spatiotemporal dependence of the lateral lipid diffusion obtained by SPT showed that the microdomain had lower fluidity than the surrounding region and worked as the obstacles for the lipid diffusion. We observed the two-dimensional assembly of FBP17, which is one of F-BAR family proteins included in endocytosis processes and has the function generating lipid bilayer tubules in vitro. At the initial stage of the FBP17 assembly, the PI-derived microdomain worked as a scaffold for the FBP17 adsorption, and the fluid surrounding region supplied FBP17 to grow the FBP17 domain via the lateral molecular diffusion. This study demonstrated an example clearly revealing the roles of two lipid microregions during the protein reaction on a lipid bilayer.

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Section: Introductionmentioning

confidence: 99%

Physical Properties and Reactivity of Microdomains in Phosphatidylinositol-Containing Supported Lipid Bilayer

et al. 2021

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“…The first visible nanomechanical parameter is related to the quantitative determination of the breakthrough event, i.e. the force threshold indicative of AFM probe penetrating the lipid layer, also confirming its existence (Butt et al, 2005;Redondo-Morata et al, 2020). Raw morphology from integrated software is representative of mica substrate, neglecting layers breaking during force curves.…”

Section: Afm Force Spectroscopymentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

“…For our study we selected one of the most studied bio-systems, represented by phospholipid bilayers as a model system mimicking real cell membrane ( Eeman and Deleu, 2010 ; Redondo-Morata et al, 2020 ). Furthermore, the modeled biomembranes can be flexibly reconstructed to facilitate surface techniques for investigation of mechanical properties such as AFM ( Stetter et al, 2016 ; Müller et al, 2020 ; Redondo-Morata et al, 2020 ), and molecular dynamic simulations ( Hofsäß et al, 2003 ; Leeb and Maibaum, 2018 ). This is significant since the biomembrane is the most critical interface between the living organisms and the external environment, while mechanical properties are correlated with its stability ( Alessandrini and Facci, 2012 ; Beedle et al, 2015 ; Balleza et al, 2019 ; Mescola et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Unveiling a Hidden Event in Fluorescence Correlative Microscopy by AFM Nanomechanical Analysis

Zhang

Wang

et al. 2021

Front. Mol. Biosci.

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Fluorescent imaging combined with atomic force microscopy (AFM), namely AFM-fluorescence correlative microscopy, is a popular technology in life science. However, the influence of involved fluorophores on obtained mechanical information is normally underestimated, and such subtle changes are still challenging to detect. Herein, we combined AFM with laser light excitation to perform a mechanical quantitative analysis of a model membrane system labeled with a commonly used fluorophore. Mechanical quantification was additionally validated by finite element simulations. Upon staining, we noticed fluorophores forming a diffuse weakly organized overlayer on phospholipid supported membrane, easily detected by AFM mechanics. The laser was found to cause a degradation of mechanical stability of the membrane synergically with presence of fluorophore. In particular, a 30 min laser irradiation, with intensity similar to that in typical confocal scanning microscopy experiment, was found to result in a ∼40% decrease in the breakthrough force of the stained phospholipid bilayer along with a ∼30% reduction in its apparent elastic modulus. The findings highlight the significance of analytical power provided by AFM, which will allow us to “see” the “unseen” in correlative microscopy, as well as the necessity to consider photothermal effects when using fluorescent dyes to investigate, for example, the deformability and permeability of phospholipid membranes.

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“…It was determined that the gold-plated sensor surface was stable before the coated process, by detecting À 0.23 (� 0.11) Hz resonance frequency shift with the measuring system. [26] Then the coating was started. The stratification stage MPS coating monitored by QCMÀ D device took 60 minutes, consequently the frequency shift due to MPS coating was measured at an average of À 5.42 (� 1.60) Hz.…”

Section: Resultsmentioning

confidence: 99%

The Effect of (3‐Mercaptopropyl)trimethoxysilane (MPS) Coating on the Genetic Detection Performance of Quartz Crystal Microbalance‐Dissipation (QCM‐D) Biosensor: Novel Intact Double‐Layered Surface Modification on QCM‐D

Soysaldı

Soylu

2021

ChemistrySelect

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Biosensor's ability depends on both platform performance and surface modification. In mass sensitive biosensors, a stable coating with molecular structural dynamism increases sensitivity. Also, its orientation and smoothness directly affect the performance of cross-linker molecules and receptors. In this study, the performance of intact double-layered (ID) coating used on QCMÀ D for the first time in DNA detection of MPS was investigated. The sensitivity of the QCMÀ D with ID-MPS was compared to the uncoated. At the concentrations of 10 10 and 10 13 copies/mL, the method with ID-MPS was approximately 6.4 times more sensitive. A shift of 1.22(� 0.42) Hz was observed at the detection limit at 10 8 copies/mL, while it was 0.19(� 0.05) Hz in the negative control. Thus, a signal-to-noise-ratio greater than 3, which is a measure of signal quality in biosensors, was exceeded with a ratio of 3.33, and it was shown that the detection signal at the sensitivity limit was sufficient. In addition, the two methods were compared visually with atomic force microscopy in terms of the amount and distribution of captured molecules, and it was determined that the ID-MPS was more efficient.

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Lipid bilayers: Phase behavior and nanomechanics

Cited by 20 publications

References 184 publications

Physical Properties and Reactivity of Microdomains in Phosphatidylinositol-Containing Supported Lipid Bilayer

Physical Properties and Reactivity of Microdomains in Phosphatidylinositol-Containing Supported Lipid Bilayer

Unveiling a Hidden Event in Fluorescence Correlative Microscopy by AFM Nanomechanical Analysis

The Effect of (3‐Mercaptopropyl)trimethoxysilane (MPS) Coating on the Genetic Detection Performance of Quartz Crystal Microbalance‐Dissipation (QCM‐D) Biosensor: Novel Intact Double‐Layered Surface Modification on QCM‐D

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