Lipid bodies: structural markers of inflammatory macrophages in innate immunity

Melo, Rossana C. N.; Fabrino, Daniela Leite; Dias, Felipe F.; Parreira, Gleydes G.

doi:10.1007/s00011-006-5205-0

Cited by 68 publications

(115 citation statements)

References 31 publications

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“…[22][23] The association between T. gondii infection and increased concentrations of proteins and lipids is consistent with a previous report where the size and number of lipid droplets, whose major component is TGAs, increased in cells infected by T. gondii. 24 The interaction of parasite proteins with these lipid bodies is important for the replication of intracellular pathogen, such as T. gondii and for the biogenesis of new parasite particles. 24 The intense bands at 788 cm -1 also indicated increased amount of nucleic acids, also confirmed by the intense AO fluorescence intensity.…”

Section: Raman Spectroscopy Of Live T Gondii During Infectionmentioning

confidence: 99%

“…24 The interaction of parasite proteins with these lipid bodies is important for the replication of intracellular pathogen, such as T. gondii and for the biogenesis of new parasite particles. 24 The intense bands at 788 cm -1 also indicated increased amount of nucleic acids, also confirmed by the intense AO fluorescence intensity. The source of the nucleic acids' traces, host or parasite origin, is unknown.…”

Section: Raman Spectroscopy Of Live T Gondii During Infectionmentioning

confidence: 99%

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Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy

Naemat

Elsheikha

Al-sandaqchi

et al. 2015

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Label-free imaging using Raman micro-spectroscopy (RMS) was used to characterize the spatio-temporal molecular changes of T. gondii tachyzoites and their host cell microenvironment. Raman spectral maps were recorded from isolated T. gondii tachyzoites and T. gondii-infected human retinal cells at 6 hr, 24 hr and 48 hr postinfection. Principal component analysis (PCA) of the Raman spectra of paraformaldehyde-fixed infected cells indicated a significant increase in the amount of lipids and proteins in the T. gondii tachyzoites as the infection progresses within host cells. These results were confirmed by experiments carried out on live T. gondii-infected cells and were correlated with an increase in the concentration of proteins and lipids required for the replication of this intracellular pathogen. These findings demonstrate the potential of RMS to characterize time-and spatially-dependent molecular interactions between intracellular pathogens and the host cells. Such information may be useful for discovery of pharmacological targets or screening compounds with potential neuroprotective activity for eminent effects of changes in brain infection control practices. 2

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Section: Raman Spectroscopy Of Live T Gondii During Infectionmentioning

confidence: 99%

Section: Raman Spectroscopy Of Live T Gondii During Infectionmentioning

confidence: 99%

Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy

Naemat

Elsheikha

Al-sandaqchi

et al. 2015

Analyst

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“…Although lipid bodies have central roles in inflammation and are considered structural markers of inflammatory cells in a range of diseases (6,10,11), their identification has methodological limitations because lipid bodies dissipate upon drying or dissolve upon fixation and staining with alcohol-based reagents (12)(13)(14). For example, May-Grunwald-Giemsa staining causes the dissolution of lipid bodies (15).…”

mentioning

confidence: 99%

“…Usually, these organelles are identified by light microscopy using lipophilic fluorescent probes (12,13,16) or staining with osmium (17). At the ultrastructural level, lipid bodies can be observed by transmission electron microscopy (TEM) without any additional labeling, by which they appear as distinct organelles lacking delimiting classical trilaminar membranes (2,10,18). However, TEM preparations are time-consuming and require costly processing, such as embedding and thin sectioning.…”

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confidence: 99%

Leukocyte lipid bodies: inflammation-related organelles are rapidly detected by wet scanning electron microscopy

Melo

Sabban²,

Weller

2006

Journal of Lipid Research

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Leukocyte lipid bodies are dynamic, functionally active organelles with central roles in inflammation. Here, we report that leukocyte lipid bodies are facilely detected by a versatile, potent technique, termed wet scanning electron microscopy (SEM), which combines the rapid preparation of light microscopy with the resolution of SEM. Using as leukocyte models resting and agonist-stimulated human eosinophils, cells that generate prominent numbers of lipid bodies in inflammatory conditions, we demonstrated that lipid bodies can be rapidly imaged as bright, highly contrasted structures under wet SEM and scored by computerized image processing. Critical advantages of this approach are that it permits cell observation in a fully hydrated system and facilitates lipid preservation. These attributes are especially important because lipid bodies are degraded during routine dehydration processes. Moreover, this technology is advantageous over lipophilic fluorescent probes because it allows sustained detection of lipid bodies in contrast to short-lived fluorescent labeling of these organelles. The value of wet SEM in enabling rapid and largescale lipid body imaging and scoring within leukocytes is particularly important because lipid bodies are organelles underlying the heightened functions of inflammatory cells. Wet SEM technology provides new approaches and opportunities for delineations of lipid bodies in inflammatory diseases, including allergic inflammation.-Melo, R. C. N., A. Sabban, and P. F. Weller. Leukocyte lipid bodies: inflammation-related organelles are rapidly detected by wet scanning electron microscopy.

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“…2003) and electron microscopy (Dvorak 2005;Dvorak et al 1983;Melo 2009;Melo, Fabrino, et al 2006;Wan et al 2007), including wet scanning electron microscopy (SEM; Melo, Sabban, et al 2006). In this review, we discuss structural and functional aspects of LBs and imaging techniques to visualize these organelles in cells associated with inflammatory responses.…”

mentioning

confidence: 99%

Lipid Bodies in Inflammatory Cells

Melo

D’Avila

Wan

et al. 2011

J Histochem Cytochem.

149

105

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Lipid bodies (LBs), also known as lipid droplets, have increasingly been recognized as functionally active organelles linked to diverse biological functions and human diseases. These organelles are actively formed in vivo within cells from the immune system, such as macrophages, neutrophils, and eosinophils, in response to different inflammatory conditions and are sites for synthesis and storage of inflammatory mediators. In this review, the authors discuss structural and functional aspects of LBs and current imaging techniques to visualize these organelles in cells engaged in inflammatory processes, including infectious diseases. The dynamic morphological aspects of LBs in leukocytes as inducible, newly formable organelles, elicitable in response to stimuli that lead to cellular activation, contribute to the evolving understanding of LBs as organelles that are critical regulators of different inflammatory diseases, key markers of leukocyte activation, and attractive targets for novel anti-inflammatory therapies.

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Lipid bodies: structural markers of inflammatory macrophages in innate immunity

Cited by 68 publications

References 31 publications

Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy

Analysis of interaction between the apicomplexan protozoan Toxoplasma gondii and host cells using label-free Raman spectroscopy

Leukocyte lipid bodies: inflammation-related organelles are rapidly detected by wet scanning electron microscopy

Lipid Bodies in Inflammatory Cells

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