Lipid droplet levels vary heterogeneously in response to simulated gastrointestinal stresses in different probiotic Saccharomyces cerevisiae strains

Zamith-Miranda, Daniel; Palma, Mauri Sérgio Alves; Matos, Gabriel S.; Schiebel, Johnathon G.; Maya‐Monteiro, Clarissa M.; Aronovich, Marcos; Bozza, Patrı́cia T.; Nimrichter, Leonardo; Montero-Lomelı́, Mónica; Marques, Ernesto T. A.; Martins, Flaviano S.; Douradinha, Bruno

doi:10.1016/j.jff.2015.12.013

Cited by 9 publications

(2 citation statements)

References 39 publications

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“…Currently, there are two main groups of analytical methods that are getting extensively utilized for rapid analysis of microbial lipids: (a) methods based on using lipophilic fluorescent dyes (Sudan black, Nile red, and BODIPY) [11–16] and, (b) methods based on flow cytometry [17]. Both methods are rapid, non-invasive and do not require tedious and time-consuming extraction of lipids, and to some extent have a possibility for high-throughput analysis.…”

Section: Introductionmentioning

confidence: 99%

Biochemical profiling, prediction of total lipid content and fatty acid profile in oleaginous yeasts by FTIR spectroscopy

Shapaval

Brandenburg

Blomqvist

et al. 2019

Biotechnol Biofuels

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Background Oleaginous yeasts are considered as a potential lipid source for food, feed and biofuel production. In order to make the yeast-based lipid production environmentally and economically sustainable, there is a need for screening studies in order to find the best yeast lipid producers on different substrates, and to optimize cultivation conditions. Since the target parameter of such screening studies are lipid amounts and profiles, an analytical technique that is able to perform lipid analyses rapidly, reproducible and with high precision is highly desirable. The main objective of this study was to establish the non-invasive high-throughput Fourier transform infrared (FTIR) spectroscopy analysis for the prediction of lipid content and profile in oleaginous yeasts. Results High-throughput FTIR spectroscopy allowed characterizing the total biochemical profile of oleaginous yeasts and enabled us to identify strains and substrate(s) providing the highest total lipid content. Some of the yeast strains grown under nitrogen-limiting conditions with glucose/xylose/mixture of glucose and xylose as carbon sources were accumulating lipids with a high proportion of free fatty acids. FTIR spectra were used to predict gravimetric and gas chromatography data by establishing multivariate calibration models. Coefficients of determination ( R 2 ) for calibration models were obtained in a range between 0.62 and 0.92 for predicting lipid content. When using an independent test set, R 2 values between 0.53 and 0.79 were achieved for predicting fatty acid profile. The best spectral region(s) for the prediction of total lipid content was 3100–2800 cm −1 combined with 1800–700 cm −1 , and for prediction of summed saturated (SAT), monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids: 3100–2800 cm −1 , 3100–2800 cm −1 combined with 1700–1715 cm −1 and 3100–2800 cm −1 combined with 1800–1715 cm −1 , respectively. The highest lipid accumulation was observed for strains Rhodotorula babjevae DBVPG 8058 on glucose and mixture of glucose and xylose and Lipomyces starkeyi CBS 2512 on xylose. Conclusions Applying FTIR spectroscopy combined with multivariate data analysis allows performing rapid, non-invasive, reproducible and precise quantitative predictions of total lipid content and lipid profile. It allows also detecting different lipid fractions as triacylglycerols (TAGs) and free fatty acids and evaluating the total biochemical profile of cells. Several yeast strains with high lipid accumulation were identified. Electronic supplementary material The online version of this article (10.1186/s13068-019-14...

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Section: Introductionmentioning

confidence: 99%

Biochemical profiling, prediction of total lipid content and fatty acid profile in oleaginous yeasts by FTIR spectroscopy

Shapaval

Brandenburg

Blomqvist

et al. 2019

Biotechnol Biofuels

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“…The bile salt resistance of S. cerevisiae could be attributed to an increase in its lipid content after being exposed to bile salts and low pH. These lipids contents probably act as a protective agent against bile salt stress [ 52 , 53 ].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Characterization and Identification of Potential Probiotic Yeasts Isolated from Fermented Dairy and Non-Dairy Food Products

Alkalbani

Osaili

Al‐Nabulsi

et al. 2022

JoF

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This study is about the isolation of yeast from fermented dairy and non-dairy products as well as the characterization of their survival in in vitro digestion conditions and tolerance to bile salts. Promising strains were selected to further investigate their probiotic properties, including cell surface properties (autoaggregation, hydrophobicity and coaggregation), physiological properties (adhesion to the HT-29 cell line and cholesterol lowering), antimicrobial activities, bile salt hydrolysis, exopolysaccharide (EPS) producing capability, heat resistance and resistance to six antibiotics. The selected yeast isolates demonstrated remarkable survivability in an acidic environment. The reduction caused by in vitro digestion conditions ranged from 0.7 to 2.1 Log10. Bile salt tolerance increased with the extension in the incubation period, which ranged from 69.2% to 91.1% after 24 h. The ability of the 12 selected isolates to remove cholesterol varied from 41.6% to 96.5%, and all yeast strains exhibited a capability to hydrolyse screened bile salts. All the selected isolates exhibited heat resistance, hydrophobicity, strong coaggregation, autoaggregation after 24 h, robust antimicrobial activity and EPS production. The ability to adhere to the HT-29 cell line was within an average of 6.3 Log10 CFU/mL after 2 h. Based on ITS/5.8S ribosomal DNA sequencing, 12 yeast isolates were identified as 1 strain for each Candida albicans and Saccharomyces cerevisiae and 10 strains for Pichia kudriavzevii.

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Microorganisms from Patagonian Aquatic Environments for Use in Aquaculture

Sequeiros

Garcés

Fernández

et al. 2016

Biology and Biotechnology of Patagonian Microorganisms

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Lipid droplet levels vary heterogeneously in response to simulated gastrointestinal stresses in different probiotic Saccharomyces cerevisiae strains

Cited by 9 publications

References 39 publications

Biochemical profiling, prediction of total lipid content and fatty acid profile in oleaginous yeasts by FTIR spectroscopy

Biochemical profiling, prediction of total lipid content and fatty acid profile in oleaginous yeasts by FTIR spectroscopy

In Vitro Characterization and Identification of Potential Probiotic Yeasts Isolated from Fermented Dairy and Non-Dairy Food Products

Microorganisms from Patagonian Aquatic Environments for Use in Aquaculture

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