Lipid Monolayer and Interaction with Anesthetics

Yamämoto, Yasushi; Taga, Keijiro

doi:10.1002/9781119075691.ch3

Cited by 4 publications

(23 citation statements)

References 75 publications

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“…In previous articles [ 16 , 37 ], more details of the monolayer preparation were presented. The experimental details of the monolayer formation have been reported previously [ 16 , 37 ].…”

Section: Methodsmentioning

confidence: 99%

“…In previous articles [ 16 , 37 ], more details of the monolayer preparation were presented. The experimental details of the monolayer formation have been reported previously [ 16 , 37 ]. After gently spreading a 1 μL droplet of the dropping solution on the water surface, the next drop was added after ≥1 min to ensure that the previous droplet components expanded sufficiently on the water surface.…”

Section: Methodsmentioning

confidence: 99%

“…A Surface Tensiometer (model: CBVP-A3, manufactured by Kyowa Interface Science Corporation Limited, Japan) attached to a platinum Wilhelmy was employed to conduct the surface tension measurement (STm) of prepared pure and mixed monolayers [ 16 , 37 ]. As mentioned in Section 2.2 , each solution for monolayer formation was spread on the purified water surface, and the surface tension (ST) was recorded after the dropping of each droplet and sufficient expansion.…”

Section: Methodsmentioning

confidence: 99%

“…Various model-biomembranes, such as monolayers and bilayers, micelles, vesicles, and liposormes, are used for the elucidation of membrane function [ 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ] including the anesthesia phenomenon. Among them, the monolayers formed on the water surface have the following characteristics: amphiphilic molecules arrange their hydrophobic moieties toward the air phase and their hydrophilic moieties toward the water subphase.…”

Section: Introductionmentioning

confidence: 99%

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The Interaction between Anesthetic Isoflurane and Model-Biomembrane Monolayer Using Simultaneous Quartz Crystal Microbalance (QCM) and Quartz Crystal Impedance (QCI) Methods

Yamamoto,

Ito,

Akatsuka

et al. 2024

Membranes

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The interaction between anesthetic Isoflurane (Iso) and model-biomembrane on the water surface has been investigated using quartz crystal microbalance (QCM) and quartz crystal impedance (QCI) methods. The model-biomembranes used were dipalmitoyl phosphatidyl choline (DPPC), DPPC-palmitic acid (PA) mixture (DPPC:PA = 8:2), DPPC-Alamethicin (Al) mixture (DPPC:Al = 39:1), and DPPC-β-Lactoglobulin (βLG) mixture (DPPC:βLG = 139:1) monolayers, respectively. The quartz crystal oscillator (QCO) was attached horizontally to each monolayer, and QCM and QCI measurements were performed simultaneously. It was found that Iso hydrate physisorbed on each monolayer/water interface from QCM and changed those interfacial viscosities from QCI. With an increase in Iso concentration, pure DPPC, DPPC-PA mixed, and DPPC-Al mixed monolayers showed a two-step process of Iso hydrate on both physisorption and viscosity, whereas it was a one-step for the DPPC-βLG mixed monolayer. The viscosity change in the DPPC-βLG mixed monolayer with the physisorption of Iso hydrate was much larger than that of other monolayers, in spite of the one-step process. From these results, the action mechanism of anesthetics and their relevance to the expression of anesthesia were discussed, based on the “release of interfacial hydrated water” hypothesis on the membrane/water interface.

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“…In previous articles [ 16 , 37 ], more details of the monolayer preparation were presented. The experimental details of the monolayer formation have been reported previously [ 16 , 37 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Interaction between Anesthetic Isoflurane and Model-Biomembrane Monolayer Using Simultaneous Quartz Crystal Microbalance (QCM) and Quartz Crystal Impedance (QCI) Methods

Yamamoto,

Ito,

Akatsuka

et al. 2024

Membranes

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show abstract

“…π increased gradually and monotonously up to 28 mN/m with completion of monolayer formation. The shapes of these isotherm curves were somewhat smooth but did not always resemble with a typical LE state such as dimyristoyl phosphatidyl choline (DMPC) monolayer [21] [22]. 1-OP had an intermediate isotherm curve between LE and LC states monolayers, a gradual increase in the beginning and subsequently a steep increase in π was noticed with monolayer formation at around 15 mN/m (Figure 2).…”

Section: Density Functional Theory (Dft) Methods Calculation With B3lymentioning

confidence: 99%

Surface Properties of Novel Surfactant, Dihexadecyl Gemini Phosphate, Monolayers on Water Surface by Dropping Method

Kobayashi¹,

Amano²,

Taga³

et al. 2017

JBPC

Self Cite

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We have synthesized first novel dihexadecyl phosphate (DHGP-n) gemini surfactants containing alkyl chain ((C n H 2n ); n = 3, 4, 5, and 6) spacers. Surfactants were used to form monolayers on water surface. Surface tension measurement (STm), Brewster angle microscopy (BAM), and density functional theory (DFT) calculation were used to investigate these monolayers. Surface pressure (π) and molecular area (A); π-A isotherm curves of all DHGP-n gemini surfactants showed a gradual increase in surface pressure without any break points in the curves, confirmed the formation of liquid-expand (LE) type monolayers. BAM observations also supported the STm results. Limited molecular area (A 0 ) of these monolayers depended on the spacer of gemini surfactants without spacer n = 4. Longer alkyl chain spacer in the series had higher value of A 0 in π-A isotherm plots. In the series, A 0 of spacer n = 4 was smallest among A 0 values of other spacers. Density functional theory (DFT) method calculation also confirmed π-A isotherm curves pattern of these surfactants. Calculation showed that both odd and even numbers and length of the alkyl chain spacer influenced the structure of DHGP-n monolayer formation on the water surface.

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Morphology Observation of Two-Dimensional Monolayers of Model Proteins on Water Surface as Revealed by Dropping Method

Asada,

Tanaka,

Nagano

et al. 2024

Bioengineering

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We have investigated the morphology of two-dimensional monolayers of gramicidin-D (GD) and alamethicin (Al) formed on the water surface by the dropping method (DM) using surface tension measurement (STm), Brewster angle microscopy (BAM), and atomic force microscopy (AFM). Dynamic light scattering (DLS) revealed that GD in alcoholic solutions formed a dimeric helical structure. According to the CD and NMR spectroscopies, GD molecules existed in dimer form in methanol and lipid membrane environments. The STm results and BAM images revealed that the GD dimer monolayer was in a liquid expanded (LE) state, whereas the Al monolayer was in a liquid condensed (LC) state. The limiting molecular area (A0) was 6.2 ± 0.5 nm2 for the GD-dimer and 3.6 ± 0.5 nm2 for the Al molecule. The AFM images also showed that the molecular long axes of both the GD-dimer and Al were horizontal to the water surface. The stability of each monolayer was confirmed by the time dependence of the surface pressure (π) observed using the STm method. The DM monolayer preparation method for GD-dimer and Al peptide molecules is a useful technique for revealing how the model biological membrane’s components assemble in two dimensions on the water surface.

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Lipid Monolayer and Interaction with Anesthetics

Cited by 4 publications

References 75 publications

The Interaction between Anesthetic Isoflurane and Model-Biomembrane Monolayer Using Simultaneous Quartz Crystal Microbalance (QCM) and Quartz Crystal Impedance (QCI) Methods

The Interaction between Anesthetic Isoflurane and Model-Biomembrane Monolayer Using Simultaneous Quartz Crystal Microbalance (QCM) and Quartz Crystal Impedance (QCI) Methods

Surface Properties of Novel Surfactant, Dihexadecyl Gemini Phosphate, Monolayers on Water Surface by Dropping Method

Morphology Observation of Two-Dimensional Monolayers of Model Proteins on Water Surface as Revealed by Dropping Method

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