Lipid nanoparticles assessment by flow cytometry

Bryła, Anna; Juzwa, Wojciech; Weiss, Marek; Lewandowicz, G.

doi:10.1016/j.ijpharm.2017.01.047

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…Cellular uptake of the PTX-Lip2N/PTX-Lip was quantitatively measured by using DiD as a fluorescent probe instead of PTX loaded in the liposomes. 31,32 Briefly, BxPC-3 cells were cultured in a 12-well plate at a density of 1×10 5 cells per well and incubated for 24 h at 37°C in a humidified atmosphere containing 5% CO 2 . After that, serum-free culture media containing free DiD, DiD-Lip and DiD-Lip2N with the same final DiD concentration of 0.5 μg/mL were applied to replace the original medium and incubated at 37°C.…”

Section: In Vitro Cellular Uptakementioning

confidence: 99%

<p>Enhancement of Antitumor Efficacy of Paclitaxel-Loaded PEGylated Liposomes by N,N-Dimethyl Tertiary Amino Moiety in Pancreatic Cancer</p>

Yang¹,

Wang²,

Luo

et al. 2020

DDDT

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Introduction: Pancreatic cancer, or pancreatic duct adenocarcinoma (PDAC), remains one of the most lethal cancers and features insidious onset, highly aggressive behavior and early distant metastasis. The dense fibrotic stroma surrounding tumor cells is thought to be a shield to resist the permeation of chemotherapy drugs in the treatment of PDAC. Thus, we synthesized a pancreas-targeting paclitaxel-loaded PEGylated liposome and investigated its antitumor efficacy in the patient-derived orthotopic xenograft (PDOX) nude mouse models of PDAC. Methods: The PTX-loaded PEGylated liposomes were prepared by film dispersionultrasonic method and modified by an N,N-dimethyl tertiary amino residue. Morphology characteristics of the PTX-loaded liposomes were observed by transmission electron microscope (TEM). The PDOX models of PDAC were established by orthotopic implantation and imaged by a micro positron emission tomography/computed tomography (PET/CT) imaging system. The in vivo distribution and antitumor study were then carried out to observe the pancreas-targeting accumulation and the antitumor efficacy of the proposed PTX liposomes. Results: PTX loaded well into both modified (PTX-Lip2N) and unmodified (PTX-Lip) PEGylated liposomes with spherical shapes and suitable parameters for the endocytosis process. The PDOX nude mouse models were successfully created in which high 18 F-FDG intaking regions were observed by micro-PET/CT. In addition to higher cellular uptakes of PTX-Lip2N by the BxPC-3 cells, the proposed nanoparticle had a notable penetrating ability towards PDAC tumor tissues, and consequently, the antitumor ability of PTX-Lip2N was significantly superior to the unmodified PTX-Lip in vivo PDOX models and even more effective than nab-PTX in restraining tumor growth. Conclusion: The modified pancreas-targeting PTX-loaded PEGylated liposomes provide a promising platform for the treatment of pancreatic cancer.

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Section: In Vitro Cellular Uptakementioning

confidence: 99%