Lipid peroxidation in liver tissue of ovariectomized and pinealectomized rats: effect of estradiol and progesterone supplementation

Abstract

The present study aimed to determine the effect of estradiol-progesterone supplementation and pinealectomy on lipid peroxidation of liver tissue in ovariectomized rats. The study was carried out on 36 adult Sprague-Dawley female rats, which weighed 200-250 g. The rats were divided into 6 groups: Group 1: Sham Ovariectomy (Sham-Ovx), Group 2: Ovariectomy (Ovx), Group 3: Ovx + Estradiol-Progesterone supplementation (Ovx + H), Group 4: Sham Pinealectomy and Ovx (Sham Pnx -Ovx), Group 5: Ovx -Pnx, Group 6: Ovx -Pnx + H. Malondialdehyde (MDA), reduced form of glutathione (GSH) and glutathione peroxidase (GSH-Px) levels were determined in liver tissue of rats. The highest MDA levels and the lowest GSH-Px levels were determined in the ovariectomized-pinealectomized group, whereas the lowest MDA was in the Sham-Ovx group, and the highest GSH-Px levels were found in the Sham-Ovx and Ovx + Hormone supplemented group. Furthermore, the highest GSH levels were in group 1 and lowest levels were in group 5. The findings of this study demonstrate that ovariectomy led to lipid peroxidation in liver tissues of rats. Pinealectomy in addition to ovariectomy, increases lipid peroxidation, but, estradiol and progesterone supplementations to the ovariectomized-pinealectomized rats protect against lipid peroxidation to a significant extent.

Estradiol affects liver mitochondrial function in ovariectomized and tamoxifen-treated ovariectomized female rats

“…4A, B, C and 5). Accordingly, recent studies have shown that although ovariectomy leads to oxidative damage in liver (Oztekin et al, 2006a) and renal tissue (Oztekin et al, 2006b), female sex hormones (E2 and progesterone) supplementation protect against lipid peroxidation by activating the antioxidant system. In the same line, it has been shown that postmenopausal women tend to have high levels of plasma malondialdehyde (MDA) that are reversed by estrogen replacement therapy (Kumru et al, 2005).…”

Relationship between Oxidative Stress and Cardiomyopathic Changes in Ovariectomized Rats

“…Estrogen by itself is a critical regulator hormone for oxidative stress in that it alters serum lipid concentrations, coagulation and fibrinolytic systems, antioxidant systems and the production of other vasoactive molecules, such as nitric oxide and prostaglandins, all of which can influence the development of vascular disease [5]. It is known that sex hormones are associated with oxidative status and lipid peroxidation (LPO) [6,7,8,9]. Many studies have used ovariectomized rat models to demonstrate the relationship between decreased sex hormones and oxidative stress in various tissues and focused on LPO.…”

The Effect of Long-Term Controlled Fasting (The Ramadan Model) on Body Mass Index, Blood Biochemistry and Oxidative Stress Factors

“…Thema jores tro geninrep ro duc ti onpe ri odoffema lesises tra di ol(E2).Inthestu di escar ri edout,it wassta tedthatinova ri ec to mi zedpa ti entsE2tre -at mentre du cedthein cre a singoxi da ti vestressand func ti o nedasanan ti o xi dantthankstothisfe a ture. 37,38 Phe no licstruc tu reofes tro genwaspo in ted outasare a sonofthisef fect. 39 Yet,onthecon trary, inanex pe ri men talstudyad mi nis te redbyAy di lek etal.itwasex pres sedthatthetes tos te ro ne,which isado mi nanthor mo neinma les,in cre a sedoxi dati vestressanddec re a sedGSH.…”