Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes

Fraga, César G.; Leibovitz, Brian; Tappel, Al L.

doi:10.1016/0891-5849(88)90023-8

Cited by 656 publications

(339 citation statements)

References 15 publications

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“…47,48) Another possibility for the corrective role of this formulated drug on testis may be the protection in testicular oxidative stress noted here in diabetic state by the measurement of testicular catalase and peroxidase activities, important scavenging enzymes of reactive oxygen species, 49) and quantification of testicular TBARS and CD, the products of free radical. 50) The sperm count, which was decreased in diabetes, may be due to inhibition in spermatogenesis as reflected here by the quantification of different generation of germ cells at stage VII of spermatogenic cycle, an important reflector of holistic approach of spermatogenesis. 51) Another possibility of low sperm count may be the effect of testicular oxidative stress in diabetic state as reported by other.…”

Section: Discussionmentioning

confidence: 99%

Protection of Testicular Dysfunctions by MTEC, a Formulated Herbal Drug, in Streptozotocin Induced Diabetic Rat

Mallick

Mandal

Barik

et al. 2007

Biological & Pharmaceutical Bulletin

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Diabetes is associated with declining sexual function in both male 1) and female 2) individuals. In case of female, diabetic symptoms consistent with autonomic neuropathy associated with decreased subjective sexual arousal. 3) In recent study Erol et al. noted that women with type 2 diabetes experienced higher prevalence rates of sexual dysfunction when compared to non diabetic. 4) Approximately 80% of diabetic patients complained loss of libido, 60% had diminished clitoral sensitivity, 50% experienced organic dysfunction, 40% experienced vaginal discomfort, and 40% experienced vaginal dryness. 5) Type 1 diabetic women experienced higher prevalence of reduced vasocongestion and reduced vaginal lubrication to erotic stimuli. 6) Thus these clinical studies suggest that both type 1 and type 2 impede the sexual arousal responses in women.Male reproductive alterations have been widely reported in model animals and human with diabetes. 7) Streptozotocin induced diabetes in male rats resulted atrophy of sex organ, changes in histoarchitecture of ventral prostate, 8) diminution in sperm count, 8) along with low levels in plasma gonadotrophins 9,10) and testosterone. 11) The copulatory behavior of diabetic rat has been collapsed 12) along with low fertility in induced normal mating. 12) In human, diabetes is associated with erectile dysfunction 13,14) along with loss of libido 1,5) and abnormal morphology of sperm, low plasma levels of gonadotrophins and testosterone. 15) Though there are several reports about dysfunctions of the male reproductive activity in diabetic state but the data are confusing, and the exact role that insulin plays in the regulation of male reproductive function is still unclear.In traditional practice, medicinal plants are used in many countries to control diabetes mellitus. The National Centre for Complementary and Alternative Medicine, established in 1998 by the United States Government where development of herbal medicine is one of the important subjects of study. 16,17) Moreover the herbal drug is of first choice at present because plant drugs are to be less toxic and more free from side effect than synthetic one. 18) M. paradisiaca is a tree like herb belonging to the Musaceae family and different parts of the plant have medicinal value. 19,20) T. indica LINN is tree type of plant belonging to the Caesalpiniaceae family was used as a traditional medicine for the management of diabetes mellitus. 21) E. Jambolana is used as traditional medicine for the management of diabetes. 19) This plant is found all over India and belongs to the Myrtaceae family. Jambu bark cure hemorrhages, burning sensation, dysentery, diarrhea, diabetes, excessive thirst, dyspepsia, cough and asthma and is used in preparation of astringent decoction for gargles and washes. 20) Seeds are used for the treatment of diabetes. 20) Coccinia indica is under Cucurbitaceae family and has medicinal importance as observed earlier by others. 22) In our previous work we have reported the antidiabetic effect of some of these plant pa...

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Section: Discussionmentioning

confidence: 99%

Protection of Testicular Dysfunctions by MTEC, a Formulated Herbal Drug, in Streptozotocin Induced Diabetic Rat

Mallick

Mandal

Barik

et al. 2007

Biological & Pharmaceutical Bulletin

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“…The lipid peroxidation indices namely TBARS [16] and lipid hydroperoxides [17] were estimated in plasma and liver homogenate.…”

Section: Assay Of Lipid Peroxidationmentioning

confidence: 99%

Antioxidant Effect of Caffeic Acid on Oxytetracycline Induced Lipid Peroxidation in Albino Rats

Rajendran

Subash

2010

Ind J Clin Biochem

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Caffeic acid is a well-known phenolic compound widely present in plant kingdom. The aim of this study was to investigate the possible protective effect of caffeic acid (CA) against oxytetracycline (OXT) induced hepatotoxicity in male Albino Wistar rats. A total of 30 rats weighing 150-170 g were randomly divided into five groups of six rats in each group. Oral administration of OXT (200 mg/kg body weight/day) for 15 days produced hepatic damage as manifested by a significant increase in serum hepatic markers namely aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), bilirubin and increased plasma and hepatic lipid peroxidation indices (TBARS and hydroperoxide). The present finding shows that the levels of enzymatic antioxidants namely superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were significantly decreased in OXT intoxicated rats. Upon oral administration of caffeic acid (40 mg/kg body weight/day) there were decreased hepatic marker activities, bilirubin and lipid peroxidation and increased enzymatic antioxidants in OXT ? Caffeic acid group compared to Normal ? OXT group(P \ 0.05). Our study suggests that caffeic acid has antioxidant property and hepatoprotective ability against OXT induced toxicity.

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“…The extent of lipid peroxidation was evaluated as 2-thibarbituric acid-reactive substances (TBARS) (Fraga et al, 1988). The measurement of TBARS formation was performed as described in Ohyashiki et al (1991) with some modification.…”

Section: Determination Of 2-thiobarbituric Acid-reactive Substances (mentioning

confidence: 99%

The Protective Effects of Insulin on Hydrogen Peroxide-Induced Oxidative Stress in C6 Glial Cells

Mahesh¹,

Kim²

2009

Biomolecules and Therapeutics

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-Insulin appears to play a role in brain physiology, and disturbances of cerebral insulin signalling and glucose homeostasis are implicated in brain pathology. The objective of the present study was to investigate the protective effects of insulin under conditions of oxidative stress induced by hydrogen peroxide (H2O2) in C6 glial cells. Insulin at concentration of 10 −7 M could prevent 12 h H2O2-induced cell death. The formation of reactive oxygen species (ROS), nitric oxide (NO) and 2-thiobarbituric acid-reactive substances (TBARS) were significantly scavenged by insulin pre-treatment in C6 glial cells after H 2 O 2 -induced oxidative stress. Insulin significantly stimulated the phosphorylation of Akt in the cells and the activation of Akt was maintained in response to insulin under H2O2 incubation for 12 h. In conclusion, these results provide evidence that insulin acts as a free radical scavenger and stimulating Akt activity. These data suggest that insulin may be effective in degenerative diseases with oxidative stress.

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Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes

Cited by 656 publications

References 15 publications

Protection of Testicular Dysfunctions by MTEC, a Formulated Herbal Drug, in Streptozotocin Induced Diabetic Rat

Protection of Testicular Dysfunctions by MTEC, a Formulated Herbal Drug, in Streptozotocin Induced Diabetic Rat

Antioxidant Effect of Caffeic Acid on Oxytetracycline Induced Lipid Peroxidation in Albino Rats

The Protective Effects of Insulin on Hydrogen Peroxide-Induced Oxidative Stress in C6 Glial Cells

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