Lipid peroxidation measurement by thiobarbituric acid assay in rat cerebellar slices

García, Y; Rodrı́guez-Malaver, Antonio J.; Peñaloza, Nancy

doi:10.1016/j.jneumeth.2004.10.018

Cited by 175 publications

(93 citation statements)

References 31 publications

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“…Thereafter, 0.5 ml of 30% trichloroacetic acid (TCA) and 0.5 ml of 52mM thiobarbituric acid (TBA) was added to the mixture. Desferal was added to prevent further iron-catalyzed TBARS formation [5] and BHT was used to prevent further lipid peroxidation [10]. The reaction mixture was processed for the measurement of TBARS as described [30].…”

Section: Assay Methodsmentioning

confidence: 99%

Protective effect of arginine on oxidative stress in transgenic sickle mouse models

Dasgupta

Hebbel

Kaul

2006

Free Radical Biology and Medicine

129

101

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Sickle cell disease (SCD) is characterized by reperfusion injury and chronic oxidative stress. Oxidative stress and hemolysis in SCD result in inactivation of nitric oxide (NO) and depleted arginine levels. We hypothesized that augmenting NO production by arginine supplementation will reduce oxidative stress in SCD. To this end, we measured the effect of arginine (5% in mouse chow) on NO metabolites (NOx), lipid peroxidation (LPO) and selected antioxidants in transgenic sickle mouse models. Untreated transgenic sickle (NY1DD) mice (expressing ~75% β S -globin of all β-globins; mild pathology) and knockout sickle (BERK) mice (expressing exclusively hemoglobin S; severe pathology) showed reduced NOx levels and significant increases in the liver LPO compared with C57BL mice, with BERK mice showing maximal LPO increase in accordance with the disease severity. This was accompanied by reduced activity of antioxidants (glutathione [GSH], total superoxide dismutase [SOD], catalase and glutathione peroxidase [GPx]). However, GSH levels in BERK were higher than in NY1DD mice indicating a protective response to greater oxidative stress. Importantly, dietary arginine significantly increased NOx levels, reduced LPO and increased antioxidants in both sickle mouse models. In contrast, L-NAME, a potent non-selective NOS inhibitor, worsened the oxidative stress in NY1DD mice. Thus, attenuating effect of arginine on oxidative stress in SCD mice suggests its potential application in the management of this disease.

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Section: Assay Methodsmentioning

confidence: 99%

Protective effect of arginine on oxidative stress in transgenic sickle mouse models

Dasgupta

Hebbel

Kaul

2006

Free Radical Biology and Medicine

129

101

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“…The end products of lipid peroxidation were assessed through the levels of TBARS, a well-known biomarker of lipid peroxidation and oxidative stress (15). Figure 3 shows TBARS levels in vastus lateralis homogenates of rats subjected to acute physical exercise in the absence or presence of SF treatment.…”

Section: Figures 1 Andmentioning

confidence: 99%

Sulforaphane treatment protects skeletal muscle against damage induced by exhaustive exercise in rats

Malaguti

Angeloni

Garatachea

et al. 2009

Journal of Applied Physiology

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“…5) Lipid peroxidation by reactive oxygen species (ROS) is known to be involved in the damaging mechanism of several acute maladies such as hyperbaric oxygenation and brain ischemia as well as in chronic brain disorders such as Parkinson's and Alzheimer's diseases. 6) The most common assay currently used as an index for lipid peroxidation in biological systems is measurement of thiobarbituric acid-reactive substances (TBARS) upon the formation of a red adduct between thiobarbituric acid (TBA) and malondialdehyde (MDA). 7) The traditional use of medicinal plants in different maladies, such as reproductive-and fertilityrelated symptoms, is widespread throughout the world, especially in Asian countries.…”

Section: Introductionmentioning

confidence: 99%

Impact of Pueraria candollei Root Cultures on Cytochrome P450 2B9 Enzyme and Lipid Peroxidation in Mice

Udomsuk

Churikawit

Putalun

et al. 2010

JOURNAL OF HEALTH SCIENCE

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Pueraria candollei (Leguminosae) has long been used as a traditional medicine for rejuvenation, and the major constituents in the tuberous root are isoflavonoids. The effects of the root cultures of P. candollei (r-PC) on cytochrome P450 enzymes in mouse liver and antioxidant activity in mouse brain including gross anatomy were examined. r-PC significantly enlarged the uterus length while no change in weight was noted. The total P450 content was not significantly altered by either estradiol benzoate (ES) or r-PC. Benzyloxyresorufin O-dealkylase activity was significantly increased by r-PC, comparable to the increase induced by ES. Correspondingly, r-PC demonstrated a tendency to upregulate CYP2B9 mRNA expression. Furthermore, assessment of lipid peroxidation in mouse brain using the thiobarbituric acid assay demonstrated that r-PC remarkably decreased the level of malondealdehyde formation. These observations suggest r-PC as a potential alternative to ES with the extrabenefit of its antioxidant activity.

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Lipid peroxidation measurement by thiobarbituric acid assay in rat cerebellar slices

Cited by 175 publications

References 31 publications

Protective effect of arginine on oxidative stress in transgenic sickle mouse models

Protective effect of arginine on oxidative stress in transgenic sickle mouse models

Sulforaphane treatment protects skeletal muscle against damage induced by exhaustive exercise in rats

Impact of Pueraria candollei Root Cultures on Cytochrome P450 2B9 Enzyme and Lipid Peroxidation in Mice

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