Lipid production from a mixture of sugarcane top hydrolysate and biodiesel-derived crude glycerol by the oleaginous red yeast, Rhodosporidiobolus fluvialis

Poontawee, Rujiralai; Yongmanitchai, Wichien; Limtong, Savitree

doi:10.1016/j.procbio.2017.11.020

Cited by 49 publications

(34 citation statements)

References 43 publications

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“…The oleaginous red yeast, Rhodosporidiobolus fluvialis DMKU-SP314 was proved as a potential lipid producer [9,13]. This strain accumulated a high quantity of lipid and its fatty acid profile was similar to the profiles of plant oils, it mainly consists of oleic acid (36.6%), palmitic acid (30.6%), and linoleic acid (22.7%).…”

Section: Introductionmentioning

confidence: 99%

“…This strain accumulated a high quantity of lipid and its fatty acid profile was similar to the profiles of plant oils, it mainly consists of oleic acid (36.6%), palmitic acid (30.6%), and linoleic acid (22.7%). It produced a high lipid content of 75% of dry cell mass (18.2 g/L lipid and 24.3 g/L cell mass) by batch cultivation in a 2 L stirred-tank fermenter using a mixture of sugarcane top hydrolysate and biodiesel-derived crude glycerol as substrates [9,13]. Sugarcane top is a lignocellulosic biomass, which is very low in cost and plentiful in Thailand because it is the main byproduct of the sugarcane industry and is usually simply left in the field after cane harvesting.…”

Section: Introductionmentioning

confidence: 99%

“…Sugarcane top is a lignocellulosic biomass, which is very low in cost and plentiful in Thailand because it is the main byproduct of the sugarcane industry and is usually simply left in the field after cane harvesting. Poontawee et al [13] reported that this byproduct had to be potentially used as raw materials for lipid production by R. fluvialis DMKU-SP314. Moreover, crude glycerol, a byproduct of biodiesel production, could be used as co-substrate with hydrolysate of sugarcane top by this strain, which greatly reduces the overall cost of lipid production.…”

Section: Introductionmentioning

confidence: 99%

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Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

Poontawee

2020

Microorganisms

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Microbial lipids are able to produce from various raw materials including lignocellulosic biomass by the effective oleaginous microorganisms using different cultivation processes. This study aimed to enhance microbial lipid production from the low-cost substrates namely sugarcane top hydrolysate and crude glycerol by Rhodosporidiobolus fluvialis DMKU-SP314, using two-stage fed-batch cultivation with different feeding strategies in a 3 L stirred-tank fermenter. The effect of two feeding strategies of 147.5 g/L crude glycerol solution was evaluated including pulse feeding at different starting time points (48, 24, and 72 h after initiation of batch operation) and constant feeding at different dilution rates (0.012, 0.020, and 0.033 h−1). The maximum lipid concentration of 23.6 g/L and cell mass of 38.5 g/L were achieved when constant feeding was performed at the dilution rate of 0.012 h−1 after 48 h of batch operation, which represented 1.24-fold and 1.27-fold improvements in the lipid and cell mass concentration, respectively. Whereas, batch cultivation provided 19.1 g/L of lipids and 30.3 g/L of cell mass. The overall lipid productivity increased to 98.4 mg/L/d in the two-stage fed-batch cultivation. This demonstrated that the two-stage fed-batch cultivation with constant feeding strategy has the possibility to apply for large-scale production of lipids by yeast.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

Poontawee

2020

Microorganisms

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“…through biological processes [4,[12][13][14][15]. In terms of microbial oil production, many studies have reported the growth of microorganisms on pure glycerol, crude glycerol, and glycerol-glucose mixture to produce health-beneficial fatty acids, such as arachidonic acid and eicosapentaenoic acid, as well as mixed microbial oils as fuel precursors [14][15][16][17][18][19][20][21].…”

Section: Practical Applicationsmentioning

confidence: 99%

“…Indeed, as a biodegradable solvent, glycerol has been used to synthesize a number of bioproducts such as microbial oils (including essential polyunsaturated fatty acids), succinic acid, 1,3‐propanediol, and ethanol through biological processes . In terms of microbial oil production, many studies have reported the growth of microorganisms on pure glycerol, crude glycerol, and glycerol–glucose mixture to produce health‐beneficial fatty acids, such as arachidonic acid and eicosapentaenoic acid, as well as mixed microbial oils as fuel precursors .…”

Section: Introductionmentioning

confidence: 99%

Co‐utilization of acidified glycerol pretreated‐sugarcane bagasse for microbial oil production by a novel Rhodosporidium strain

Hassanpour

Cai

Gebbie

et al. 2019

Engineering in Life Sciences

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Acidified glycerol pretreatment is very effective to deconstruct lignocellulosics for producing glucose. Co‐utilization of pretreated biomass and residual glycerol to bioproducts could reduce the costs associated with biomass wash and solvent recovery. In this study, a novel strain Rhodosporidium toruloides RP 15, isolated from sugarcane bagasse, was selected and tested for coconversion of pretreated biomass and residual glycerol to microbial oils. In the screening trails, Rh. toruloides RP 15 demonstrated the highest oil production capacity on glucose, xylose, and glycerol among the 10 strains. At the optimal C:N molar ratio of 140:1, this strain accumulated 56.7, 38.3, and 54.7% microbial oils based on dry cell biomass with 30 g/L glucose, xylose, and glycerol, respectively. Furthermore, sugarcane bagasse medium containing 32.6 g/L glucose from glycerol‐pretreated bagasse and 23.4 g/L glycerol from pretreatment hydrolysate were used to produce microbial oils by Rh. toruloides RP 15. Under the preliminary conditions without pH control, this strain produced 7.7 g/L oil with an oil content of 59.8%, which was comparable or better than those achieved with a synthetic medium. In addition, this strain also produced 3.5 mg/L carotenoid as a by‐product. It is expected that microbial oil production can be significantly improved through process optimization.

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Next‐generation biofuels and platform biochemicals from lignocellulosic biomass

Okolie

Mukherjee

Nanda

et al. 2021

Intl J of Energy Research

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Rapid industrialization, increasing fuel prices, exhausting fossil fuel resources and greenhouse gas emissions are some of the factors instilling the search for alternative sources of energy and chemicals. Lignocellulose biomass-derived biofuels and biochemicals have emerged as clean products to complement fossil-based resources and reduce environmental impacts. Lignocellulosic biomasses are renewable, inexpensive and abundantly available resources to produce a wide variety of liquid, gaseous and solid biofuels and industrially relevant biochemicals. Different biological (e.g., fermentation and anaerobic digestion), thermochemical (e.g., liquefaction, gasification and pyrolysis) and catalytic (e.g., transesterification) conversion technologies can be used to produce fuel and chemical products from lignocellulosic biomass. This article makes a comprehensive review of different biofuels (e.g., biodiesel, bio-oil, bioethanol, biobutanol, biogas, hydrogen, syngas and jetfuel) and value-added biochemicals (e.g., propylene, ethylene, benzene, 5-hydroxymethylfurfural, levulinic acid, succinic acid, maleic acid, fumaric acid, phenols and other aromatic compounds) from lignocellulosic biomass. Additionally, the current status, challenges and future perspectives for the production and utilization of biofuels and biochemicals are systematically discussed.

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Lipid production from a mixture of sugarcane top hydrolysate and biodiesel-derived crude glycerol by the oleaginous red yeast, Rhodosporidiobolus fluvialis

Cited by 49 publications

References 43 publications

Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis

Co‐utilization of acidified glycerol pretreated‐sugarcane bagasse for microbial oil production by a novel Rhodosporidium strain

Next‐generation biofuels and platform biochemicals from lignocellulosic biomass

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