Lipid productivity and cell wall ultrastructure of six strains of Nannochloropsis: Implications for biofuel production and downstream processing

Beacham, Tracey A.; Bradley, Claire; White, Daniel A.; Bond, Peter; Ali, S. Twareque

doi:10.1016/j.algal.2014.09.003

Cited by 69 publications

(38 citation statements)

References 26 publications

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“…The strain investigated in this study showed an analogous total lipid percentage, which was in accordance with previous results. The EPA content of the studied N. gaditana strain (7.4-28.8%) was found to be comparatively superior to that of the previously mentioned species of this genus [7,14,15].…”

Section: Resultsmentioning

confidence: 95%

A euryhaline Nannochloropsis gaditana with potential for nutraceutical (EPA) and biodiesel production

et al. 2015

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Section: Resultsmentioning

confidence: 95%

A euryhaline Nannochloropsis gaditana with potential for nutraceutical (EPA) and biodiesel production

et al. 2015

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“…Nannochloropsis is a genus of unicellular Eustigmatophyceae found in various habitats (Beacham et al, 2014). Currently, draft whole-genome sequences of N. oceanica CCMP1779 (Vieler et al, 2012) and Nannochloropsis gaditana CCMP526 (Radakovits et al, 2012) have been assembled and several genetic engineering tools established.…”

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confidence: 99%

Mechanisms of Phosphorus Acquisition and Lipid Class Remodeling under P Limitation in a Marine Microalga

et al. 2017

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. Previous studies point to P limitation-induced changes in lipid composition. As, in microalgae, the molecular mechanisms of this specific P stress adaptation remain unresolved, we reveal a detailed phospholipid-recycling scheme in Nannochloropsis oceanica and describe important P acquisition genes based on highly corresponding transcriptome and lipidome data. Initial responses to P limitation showed increased expression of genes involved in P uptake and an expansion of the P substrate spectrum based on purple acid phosphatases. Increase in P trafficking displayed a rearrangement between compartments by supplying P to the chloroplast and carbon to the cytosol for lipid synthesis. We propose a novel phospholipid-recycling scheme for algae that leads to the rapid reduction of phospholipids and synthesis of the P-free lipid classes. P mobilization through membrane lipid degradation is mediated mainly by two glycerophosphoryldiester phosphodiesterases and three patatin-like phospholipases A on the transcriptome level. To compensate for low phospholipids in exponential growth, N. oceanica synthesized sulfoquinovosyldiacylglycerol and diacylglyceroltrimethylhomoserine. In this study, it was shown that an N. oceanica strain has a unique repertoire of genes that facilitate P acquisition and the degradation of phospholipids compared with other stramenopiles. The novel phospholipid-recycling scheme opens new avenues for metabolic engineering of lipid composition in algae.Phosphorus (P) availability is often a limiting factor in aquatic ecosystems (Van Mooy et al., 2009). In some microalgae, nutrient starvation limits cell division and leads to the accumulation of organic carbon (e.g. in the form of triacylglycerols [TAGs]). To cope with P limitation, the interplay of P recycling and changes in lipid classes is observed in the open sea and in culture studies of several stramenopiles and green algae (Van Mooy et al., 2009;Martin et al., 2011;Cañavate et al., 2017b). These studies showed a taxonomically diverse lipid response under P stress with unresolved questions related to the diversified mechanism behind the lipid responses. So far, only cursory insights into the lipidremodeling process have been provided by either transcriptomic or lipidomic studies on P limitation (Riekhof et al., 2005;Cruz de Carvalho et al., 2016;Shemi et al., 2016;Cañavate et al., 2017b). To capture the taxonomically diverse response and niche partitioning, studies include ecological successful algae, such as Emiliania huxleyi and Thalassiosira pseudonana (Lessard et al., 2005;Van Mooy et al., 2009), and algae featuring high resilience and lipid content during low P, like Phaeodactylum tricornutum and Nannochloropsis spp. (Rodolfi et al., 2009;Yang et al., 2013;Mayers et al., 2014;Abida et al., 2015). During these studies, focus is often on changes of long-chain fatty acids (FAs) and their contribution to lipid classes in specific pathways [OPEN] Articles can be viewed without a subscription. www.plantphysiol.org/cgi

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“…Several species in the genus Nannochloropsis are recognized as oleaginous algae owing to their ability to accumulate large quantities of lipid. However, there is inter-and intra-species variation in the lipid productivity and fatty acid composition (Ma et al 2014, Beacham et al 2014. In a study of nine Nannochloropsis strains, specific growth rates were found to range from 0.07 to 0.21 day -1 while the lipid content varied from 37 to 60% of dry weight (Ma et al 2014).…”

Section: Enhancing the Lipid Profile In Nannochloropsis: Strain Choicmentioning

confidence: 99%

“…The most suitable strain depends on the desired product; strains for fish oil production should have high levels of EPA either in polar lipids for good bioavailability or as neutral TAGs for ease of extraction, whereas those for biodiesel need shorter saturated and monounsaturated fatty acids in TAGs. Significant variation is also seen in cell wall thickness among Nannochloropsis species, which could impact on both the efficiency and cost of lipid extraction, as well as the ease with which a strain can be genetically engineered (Beacham et al 2014). Furthermore the salinity of the culture medium also influences the thickness of the wall within a species, so growth conditions could be optimized to favour downstream processing (Beacham et al 2014).…”

Section: Enhancing the Lipid Profile In Nannochloropsis: Strain Choicmentioning

confidence: 99%

The biotechnological potential of Nannochloropsis

Al-Hoqani¹,

Young²,

Purton³

2017

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Oleaginous microalgae have commercial potential as photoautotrophic cell factories capable of producing advanced biofuels and high-value speciality oils. One genus of particular interest is Nannochloropsis, which includes a number of robust marine species well suited to industrial-scale cultivation. Advances in bioprocess technology, together with strain enhancement through traditional mutagenesis or genetic engineering approaches, now offer the possibility of improving the economics of oil production from Nannochloropsis. In this review we describe the current and potential industrial applications of this genus, consider the present status of genetic enhancement methods, and highlight the need for new advances in this area -including the development of techniques for engineering the chloroplast genome.

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Lipid productivity and cell wall ultrastructure of six strains of Nannochloropsis: Implications for biofuel production and downstream processing

Cited by 69 publications

References 26 publications

A euryhaline Nannochloropsis gaditana with potential for nutraceutical (EPA) and biodiesel production

A euryhaline Nannochloropsis gaditana with potential for nutraceutical (EPA) and biodiesel production

Mechanisms of Phosphorus Acquisition and Lipid Class Remodeling under P Limitation in a Marine Microalga

The biotechnological potential of Nannochloropsis

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