Lipidomic analysis revealed n‐3 polyunsaturated fatty acids suppressed choroidal thinning and myopia progression in mice

Mori, Kiwako; Kuroha, Sayoko; Hou, Jing; Jeong, Heonuk; Ogawa, Mamoru; Ikeda, Shin‐ichi; Kang, Jing; Negishi, Kazuno; Torii, Hidemasa; Arita, Makoto; Kurihara, Toshihide; Tsubota, Kazuo

doi:10.1096/fj.202101947r

Cited by 19 publications

(8 citation statements)

References 69 publications

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“…In addition, the effect of dietary supplementation of n-3 PUFAs on myopia, a condition that is increasing worldwide, was investigated in experiments conducted on mice, in particular on a model of lensinduced myopia (LIM). The results obtained showed that EPA and its metabolites are capable of inhibiting the choroidal thinning and the myopia progression [176].…”

Section: Curcumin and Quercetinmentioning

confidence: 96%

Potential Properties of Natural Nutraceuticals and Antioxidants in Eye Protection

Maiuolo¹,

Bulotta²,

Oppedisano³

et al. 2022

Preprint

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Eye health is crucial and the onset of diseases can reduce vision and affect the quality of life of patients. The main causes of progressive and irreversible vision loss include various pathologies such as cataracts, ocular atrophy, corneal opacity, age-related macular degeneration, uncorrected refractive error, posterior capsular opacification, uveitis, glaucoma, diabetic retinopathy, retinal detachment, undetermined disease and other disorders involving oxidative stress and inflammation. The eyes are constantly exposed to the external environment and, for this reason, must be protected from damage from the outside. Many drugs, including cortisonics and antinflammatory drugs have widely been used to counteract eye disorders. However, recent advances have been obtained via supplementation of patients with natural antioxidants and nutraceuticals. In particular, evidence has been accumulated that polyphenols (mostly deriving from Citrus Bergamia) represent a reliable source of antioxidants able to counteract oxidative stress accompanying early stages of eye diseases. Luteolin, in particular, has been found to protect foto-receptors thereby improving vision in many disease states. Moreover, a consistent anti-inflammatory response was found to occur when curcumin is used alone or in combination with other nutraceuticals. On the other hand, CoQ10 has been demonstrated to produce consistent effect in reducing ocular pressure thereby leding to protection in patients undergoing glaucoma. Finally, both grape seed extract rich in anthocyanosides and polynsatured fatty acids (PUFAs) seem to contribute in the prevention of retinal disorders. Thus, combination of nutraceuticals and anti-oxidants may represent the right solution for a multiaction activity in eye protection to be associated to current drug therapies, and this will be of potential interest in early stages of eye disorders.

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Section: Curcumin and Quercetinmentioning

confidence: 96%

Potential Properties of Natural Nutraceuticals and Antioxidants in Eye Protection

Maiuolo¹,

Bulotta²,

Oppedisano³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The flow rate and column temperature were set to 0.3 mL/min and 45°C, respectively. Data‐dependent MS/MS acquisition mode was applied as previously described 17 . Briefly, the temperature and ion spray voltage floating were set to 250°C(+)/300°C(−) and −5.5(+)/4.5(−) kV, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Data-dependent MS/MS acquisition mode was applied as previously described. 17 Briefly, the temperature and ion spray voltage floating were set to 250°C(+)/300°C(−) and −5.5(+)/4.5(−) kV, respectively. The accumulation times for MS1 and MS/MS were set to 250(+)/200(−) ms and 100(+)/70(−) ms, respectively, for scanning a mass range from m/z 70 to m/z 1250(+)/1750(−).…”

Section: Liquid Chromatography-mass Spectrometry-based Lipidomicsmentioning

confidence: 99%

Long chain acyl‐CoA synthetase 6 facilitates the local distribution of di‐docosahexaenoic acid‐ and ultra‐long‐chain‐PUFA‐containing phospholipids in the retina to support normal visual function in mice

Kuroha,

Katada,

Isobe

et al. 2023

The FASEB Journal

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Docosahexaenoic acid (DHA) and ultra‐long‐chain polyunsaturated fatty acids (ULC–PUFAs) are uniquely enriched in membrane phospholipids of retinal photoreceptors. Several studies have shown that di‐DHA‐ and ULC–PUFA‐containing phospholipids in photoreceptors have an important role in maintaining normal visual function; however, the molecular mechanisms underlying the synthesis and enrichment of these unique lipids in the retina, and their specific roles in retinal function remain unclear. Long‐chain acyl‐coenzyme A (CoA) synthetase 6 (ACSL6) preferentially converts DHA into DHA–CoA, which is a substrate during DHA‐containing lipid biosynthesis. Here, we report that Acsl6 mRNA is expressed in the inner segment of photoreceptor cells and the retinal pigment epithelial cells, and genetic deletion of ACSL6 resulted in the selective depletion of di‐DHA– and ULC–PUFA‐containing phospholipids, but not mono‐DHA‐containing phospholipids in the retina. MALDI mass spectrometry imaging (MALDI–MSI) revealed the selective distribution of di‐DHA– and ULC–PUFA‐containing phospholipids in the photoreceptor outer segment (OS). Electroretinogram of Acsl6−/− mice exhibited photoreceptor cell‐derived visual impairment, whereas the expression levels and localization of opsin proteins were unchanged. Acsl6−/− mice exhibited an age‐dependent progressive decrease of the thickness of the outer nuclear layers, whereas the inner nuclear layers and OSs were normal. These results demonstrate that ACSL6 facilitates the local enrichment of di‐DHA– and ULC–PUFA‐containing phospholipids in the retina, which supports normal visual function and retinal homeostasis.

show abstract

“…In addition, the effect of dietary supplementation of n-3 PUFAs on myopia, a condition that is increasing worldwide, was investigated in experiments conducted on mice modelling lens-induced myopia (LIM). The results obtained showed that EPA and its metabolites are capable of inhibiting choroidal thinning and myopia progression [ 229 ].…”

Section: Role Of Antioxidants and Nutraceuticals In Maintaining Eye H...mentioning

confidence: 99%

Potential Properties of Natural Nutraceuticals and Antioxidants in Age-Related Eye Disorders

Maiuolo

Bulotta

Oppedisano

et al. 2022

Life

View full text Add to dashboard Cite

Eye health is crucial, and the onset of diseases can reduce vision and affect the quality of life of patients. The main causes of progressive and irreversible vision loss include various pathologies, such as cataracts, ocular atrophy, corneal opacity, age-related macular degeneration, uncorrected refractive error, posterior capsular opacification, uveitis, glaucoma, diabetic retinopathy, retinal detachment, undetermined disease and other disorders involving oxidative stress and inflammation. The eyes are constantly exposed to the external environment and, for this reason, must be protected from damage from the outside. Many drugs, including cortisonics and antinflammatory drugs have widely been used to counteract eye disorders. However, recent advances have been obtained via supplementation with natural antioxidants and nutraceuticals for patients. In particular, evidence has accumulated that polyphenols (mostly deriving from Citrus Bergamia) represent a reliable source of antioxidants able to counteract oxidative stress accompanying early stages of eye diseases. Luteolin in particular has been found to protect photoreceptors, thereby improving vision in many disease states. Moreover, a consistent anti-inflammatory response was found to occur when curcumin is used alone or in combination with other nutraceuticals. Additionally, Coenzyme Q10 has been demonstrated to produce a consistent effect in reducing ocular pressure, thereby leading to protection in patients undergoing glaucoma. Finally, both grape seed extract, rich in anthocyanosides, and polynsatured fatty acids seem to contribute to the prevention of retinal disorders. Thus, a combination of nutraceuticals and antioxidants may represent the right solution for a multi-action activity in eye protection, in association with current drug therapies, and this will be of potential interest in early stages of eye disorders.

show abstract

Lipidomic analysis revealed n‐3 polyunsaturated fatty acids suppressed choroidal thinning and myopia progression in mice

Cited by 19 publications

References 69 publications

Potential Properties of Natural Nutraceuticals and Antioxidants in Eye Protection

Potential Properties of Natural Nutraceuticals and Antioxidants in Eye Protection

Long chain acyl‐CoA synthetase 6 facilitates the local distribution of di‐docosahexaenoic acid‐ and ultra‐long‐chain‐PUFA‐containing phospholipids in the retina to support normal visual function in mice

Potential Properties of Natural Nutraceuticals and Antioxidants in Age-Related Eye Disorders

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