Lipidomic analysis reveals a radiosensitizing role of gamma-linolenic acid in glioma cells

Antal, Otília; Péter, Mária; Hackler, László; Mán, Imola; Szebeni, Gábor J.; Ayaydin, Ferhan; Hidéghety, Katalin; Vı́gh, László; Kitajka, Klára; Balogh, Gábor; Puskás, László G.

doi:10.1016/j.bbalip.2015.06.003

Cited by 22 publications

(16 citation statements)

References 77 publications

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“…In recent years, it has been found that γ-linolenic acid is cytotoxic to glioma cells, and it can enhance gamma radiosensitivity [ 35 , 36 ]. It has been suggested that this effect is related to the accumulation of the toxic products of lipid peroxidation, which are cytotoxic to glioma cells.…”

Section: Biological Activity Of Evening Primrose Oil ( Omentioning

confidence: 99%

Evening Primrose (Oenothera biennis) Biological Activity Dependent on Chemical Composition

2018

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Evening primrose (Oenothera L.) is a plant belonging to the family Onagraceae, in which the most numerous species is Oenothera biennis. Some plants belonging to the genus Oenothera L. are characterized by biological activity. Therefore, studies were conducted to determine the dependence of biological activity on the chemical composition of various parts of the evening primrose, mainly leaves, stems, and seeds. Common components of all parts of the Oenothera biennis plants are fatty acids, phenolic acids, and flavonoids. In contrast, primrose seeds also contain proteins, carbohydrates, minerals, and vitamins. Therefore, it is believed that the most interesting sources of biologically active compounds are the seeds and, above all, evening primrose seed oil. This oil contains mainly aliphatic alcohols, fatty acids, sterols, and polyphenols. Evening primrose oil (EPO) is extremely high in linoleic acid (LA) (70–74%) and γ-linolenic acid (GLA) (8–10%), which may contribute to the proper functioning of human tissues because they are precursors of anti-inflammatory eicosanoids. EPO supplementation results in an increase in plasma levels of γ-linolenic acid and its metabolite dihomo-γ-linolenic acid (DGLA). This compound is oxidized by lipoxygenase (15-LOX) to 15-hydroxyeicosatrienoic acid (15-HETrE) or, under the influence of cyclooxygenase (COX), DGLA is metabolized to series 1 prostaglandins. These compounds have anti-inflammatory and anti-proliferative properties. Furthermore, 15-HETrE blocks the conversion of arachidonic acid (AA) to leukotriene A4 (LTA4) by direct inhibition of 5-LOX. In addition, γ-linolenic acid suppresses inflammation mediators such as interleukin 1β (IL-1β), interleukin 6 (IL-6), and cytokine - tumor necrosis factor α (TNF-α). The beneficial effects of EPO have been demonstrated in the case of atopic dermatitis, psoriasis, Sjögren’s syndrome, asthma, and anti-cancer therapy.

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Section: Biological Activity Of Evening Primrose Oil ( Omentioning

confidence: 99%

Evening Primrose (Oenothera biennis) Biological Activity Dependent on Chemical Composition

2018

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“…For example, accumulating evidence points to their importance in inflammation [ 24 ] and in neurodegeneration [ 25 , 26 ]. With respect to stress response, the synthesis of TG and concomitant LD biogenesis was reported to be a hallmark of mammalian cellular stress response [ 24 , 27 , 28 ] and stress response in plants [ 29 ]. In Saccharomyces cerevisiae ( S .…”

Section: Introductionmentioning

confidence: 99%

Metabolic crosstalk between membrane and storage lipids facilitates heat stress management in Schizosaccharomyces pombe

et al. 2017

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Cell membranes actively participate in stress sensing and signalling. Here we present the first in-depth lipidomic analysis to characterize alterations in the fission yeast Schizosaccharomyces pombe in response to mild heat stress (HS). The lipidome was assessed by a simple one-step methanolic extraction. Genetic manipulations that altered triglyceride (TG) content in the absence or presence of HS gave rise to distinct lipidomic fingerprints for S. pombe. Cells unable to produce TG demonstrated long-lasting growth arrest and enhanced signalling lipid generation. Our results reveal that metabolic crosstalk between membrane and storage lipids facilitates homeostatic maintenance of the membrane physical/chemical state that resists negative effects on cell growth and viability in response to HS. We propose a novel stress adaptation mechanism in which heat-induced TG synthesis contributes to membrane rigidization by accommodating unsaturated fatty acids of structural lipids, enabling their replacement by newly synthesized saturated fatty acids.

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“…In a similar fashion, PUFAs may also be administered in combination with radiotherapy to enhance the cytotoxic action of radiation on cancer cells and at the same time protect normal cells from the cytotoxic actions of radiation since PUFAs have cytoprotective action on normal cells [ 77 , 78 ].…”

Section: Discussionmentioning

confidence: 99%

Differential action of polyunsaturated fatty acids and eicosanoids on bleomycin-induced cytotoxicity to neuroblastoma cells and lymphocytes

Polavarapu¹,

Dwarakanath

Das³

2018

aoms

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IntroductionThis study was conducted to examine whether bleomycin-induced growth inhibitory action on human neuroblastoma cells (IMR-32) is influenced by anti-inflammatory metabolites of polyunsaturated fatty acids (PUFAs): lipoxin A4 (LXA4), resolvin D1 and protectin D1 in vitro.Material and methodsThe in vitro study was conducted using monolayer cultures of exponentially growing IMR-32 cells. The effects of various PUFAs and eicosanoids and anti-inflammatory metabolites of PUFAs such as lipoxin A4 (LXA4), resolvin D1 and protectin D1 on the growth of IMR-32 cells and human lymphocytes in vitro were investigated. The potential of PUFAs, eicosanoids and LXA4, resolvin D1 and protectin D1 to modify the growth inhibitory effects of bleomycin was also studied in IMR-32 cells and human lymphocytes.ResultsPUFAs inhibited the growth of IMR-32 cells (EPA > DHA = AA > GLA = ALA > DGLA = LA) significantly (p < 0.001) while prostaglandins were found to be not effective. Bleomycin-induced growth inhibitory action on IMR-32 cells was augmented by PUFAs and its metabolites (p < 0.05). PUFAs and LXA4 did not inhibit the growth of human lymphocytes and bleomycin-induced growth inhibitory action was also not enhanced by these bioactive lipids.ConclusionsBioactive lipids have differential action on normal human lymphocytes and tumor cells in vitro. The apparent lack of effect of PUFAs in combination with bleomycin on the growth of human lymphocytes in comparison to their growth inhibitory action on IMR-32 cells suggests that PUFAs can be used in combination with bleomycin to target tumor cells with little concern over this combination’s effect on the growth of human lymphocytes. Further studies are warranted to evaluate these differential effects under in vivo conditions.

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Lipidomic analysis reveals a radiosensitizing role of gamma-linolenic acid in glioma cells

Cited by 22 publications

References 77 publications

Evening Primrose (Oenothera biennis) Biological Activity Dependent on Chemical Composition

Evening Primrose (Oenothera biennis) Biological Activity Dependent on Chemical Composition

Metabolic crosstalk between membrane and storage lipids facilitates heat stress management in Schizosaccharomyces pombe

Differential action of polyunsaturated fatty acids and eicosanoids on bleomycin-induced cytotoxicity to neuroblastoma cells and lymphocytes

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