Lipidomic changes in mouse oocytes vitrified in PEG 8000-supplemented vitrification solutions

Jung, Gun Tae; Park, Dayoung; Ahn, Jeong Min; Um, Da-Eun; Shin, Hyejin; Lee, Jae Won; Kim, Ja‐Yeon; Song, Haengseok; Kim, Kwang Pyo; Lim, Hyunjung Jade

doi:10.1016/j.cryobiol.2020.11.004

Cited by 8 publications

(7 citation statements)

References 29 publications

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“…This high sensitivity of LPC is not reported in human plasma, serum and urine where they seemed to remain stable after several freezing (− 20 °C)/warming cycle 42 . LPCs are not only sensitive to the slow freezing protocol but also to vitrification 43 . Indeed, in mice oocytes after vitrification the lysophospholipid content also decreased 43 .…”

Section: Discussionmentioning

confidence: 99%

“…LPCs are not only sensitive to the slow freezing protocol but also to vitrification 43 . Indeed, in mice oocytes after vitrification the lysophospholipid content also decreased 43 . Lysophospholipids allow the production of lysophosphatidic acid by enzymes like phospholipase A1, A2 and autotaxin in the bovine embryos and/or from the bovine endometrial and ovarian cells 44,45 .…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, the great reduction in lysophosphatidylcholine evidenced in both cases might indicate the importance of these lipids for the ability to recover from the cryopreservation process. Their specific cryoprotection might be of interest and the cryoprotectant PEG8000 might be a good candidate to assess in future studies as it was able to prevent LPC degradation in murine oocytes after vitrification 43 .…”

Section: Discussionmentioning

confidence: 99%

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Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process

Idrissi

Bourhis

Lefèvre

et al. 2021

Sci Rep

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Currently, in vitro embryo production (IVP) is successfully commercially applied in cattle. However, the high sensitivity of embryos to cryopreservation in comparison to in vivo (IVD) embryos slows the dissemination of this biotechnology. Reduced cryotolerance is frequently associated with lipid accumulation in the cytoplasm mainly due to in vitro culture conditions. The objective of this study was to evaluate the lipid composition of biopsied and sexed embryos, produced either in vivo or in vitro from the same Holstein heifers before and after a slow freezing protocol. Lipid extracts were analysed by liquid chromatography-high resolution mass spectrometry, which enabled the detection of 496 features. Our results highlighted a lipid enrichment of IVP embryos in triglycerides and oxidised glycerophospholipids and a reduced abundance in glycerophospholipids. The slow freezing process affected the lipid profiles of IVP and IVD embryos similarly. Lysophosphatidylcholine content was reduced when embryos were frozen/thawed. In conclusion, the embryonic lipid profile is impacted by IVP and slow freezing protocols but not by sex. Lysophosphatidylcholine seemed highly sensitive to cryopreservation and might contribute to explain the lower quality of frozen embryos. Further studies are required to improve embryo freezability by modulating the lipidome.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process

Idrissi

Bourhis

Lefèvre

et al. 2021

Sci Rep

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“…In fact, the lipid phase transition, followed by separation, is one of the major causes of cryo-damages in lipid-rich oocytes and embryos [ 21 , 23 ]. It has also been shown that embryo and oocyte cryopreservation protocols (slow freezing and vitrification) lead to a decrease in the phospholipid and LPC content [ 3 , 24 ]. However, LPCs are precursors of lysophosphatidic acid, which is a modulator of embryos quality markers, such as insulin-like growth factor 2 receptor and placental associated 8 as well as pluripotency factors like sex-determining region Y box 2 and octamer-binding transcription factor 4, indicating that a decrease in LPC content can affect bovine embryo quality and can lead to a dysregulation of pluripotency pathway [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of the donor factors and freezing protocols on the bovine embryonic lipid profile

Idrissi¹,

Bourhis²,

Lefèvre

et al. 2021

Biology of Reproduction

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Embryo lipid profile is affected by in vitro culture conditions, that lead to an increase in lipids. Efforts have been made to optimize embryo lipid composition as it is associated with their quality. The objective of this study was to evaluate whether the diet supplementation of donor cows (n-3 or n-6 PUFA), or the slow freezing protocols (ethylene glycol sucrose EG-S vs. glycerol trehalose GLY-TRE), or the physiological stage of the donor (nulliparous heifers vs. primiparous lactating cows) may impact the bovine embryo lipid profile. Lipid extracts of 97 embryos were individually analysed by liquid chromatography-high resolution mass spectrometry, highlighting 246 lipids including 85% being overabundant in cow embryos compared to heifer embryos. Among 105 differential lipids, 72 were overabundant after EG-S protocol, including a single glycerophosphate PA(32:1) representing 27.3% of the significantly modulated lipids, suggesting that it is degraded when GLY-TRE is used. No lipids were different according to the n-3 or n-6 supplementation of the donor cows. In conclusion, the embryonic lipid profile was mainly affected by the physiological stage of the donors and the slow freezing protocols. The overabundance of lipids in lactating cow embryos and the resulting lower quality of these embryos is consistent with the lower pregnancy rate observed in cows compared to heifers. Unlike GLY-TRE protocol, EG-S freezing allowed to preserve glycerophospholipids potentially improving the slow freezing of in vitro-produced embryos. Further studies are required to modulate embryo quality and freezability by modulating the lipidome and integrating all stages of embryonic production.

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“…Desta forma, uma combinação do efeito da estimulação ovariana com gonadotrofinas (WANG et al, 2015) e condições de cultivo in vitro, como suplementação de proteína e soro nos meios de cultivo (ABE et al, 2002;BARCELÓ-FIMBRES et al, 2011;DEL COLLADO et al, 2015;VIREQUE et al, 2017), possivelmente, resultou na super-representação de PL no grupo de FIV observada no presente estudo. Além disso, a neossíntese de triglicerídeos devido à presença de soro (ABD- EL-RAZEK et al, 2000), ou uma redução na β-oxidação na mitocôndria, pode comprometer a capacidade do embrião de metabolizar lipídios (ABE et al, 2002;CROSIER et al, 2001) (JUNG et al, 2021;JUNG et al, 2014).…”

Section: Plsunclassified

O impacto da estimulação ovariana, fertilização in vitro, vitrificação de oócitos e suplementação dos meios de vitrificação com L-carnitina e ácidos graxos no perfil lipídico de oócitos e blastocistos murinos

Berteli¹

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AGREDECIMENTOSÀ Deus, minha fé que foi força, esperança e proteção.À minha orientadora Profª. Drª. Dra. Paula Andrea de Albuquerque Salles Navarro, um exemplo de profissional e ser humano a qual me espelho diariamente; pela oportunidade e por acreditar em meu potencial, pelos ensinamentos, paciência, apoio, e pela dedicação inesgotável ao meu crescimento científico e de todos que a acompanham. Certamente foi um presente tê-la como mentora. Ao meu orientador durante o período de sanduíche, Dr. David L. Keefe, um grande pesquisador e líder, pela generosidade em me oferecer o máximo de oportunidades que estão sendo fundamentais para meu aprimoramento profissional. À Dra. Fang Helen Wang, responsável pelo Laboratório de pesquisa do Dr. Keefe na New York University, pela paciência e por todo conhecimento compartilhado durante meu período de sanduíche. À Dra. Fabiana Barzotto Kohlrausch, pelos ensinamentos, parceria e amizade durante o sanduíche. Seu incentivo e apoio foram muito importantes. À Dra. Alessandra A. Vireque, pela orientação, parceria, apoio, paciência, pela amizade que este trabalho trouxe e principalmente pela grande paixão que tens pela pesquisa que me motivaram a não desistir frente aos desafios e frustrações. À Caroline Mantovani da Luz que caminhou comigo nesta jornada, partilhando das lutas, frustrações e vitórias nesta longa trajetória de experimentos. Obrigada pelo incentivo, apoio e amizade ao longo destes anos. Ao Eduardo Domingos Borges por todo auxílio com o trabalho, pelo incentivo e amizade que foram essenciais para que este trabalho fosse concluído. À Dra. Christina Ramires Ferreira pelo conhecimento compartilhado, apoio e análises que foram realizadas na Purdue University sob sua supervisão -EUA. À Fernanda Veronese de Oliveira que participou ativamente do início desta jornada, meu muito obrigado pelo apoio e amizade. À Marília Alves e a Maria Aparecida Vasconcelos pela ajuda nos experimentos. Obrigada pela amizade e colaboração.A todos os amigos da pós-graduação, obrigada pelo carinho, amizade e por todos os momentos de descontração.A todas as funcionárias e amigas do laboratório de GO:

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Lipidomic changes in mouse oocytes vitrified in PEG 8000-supplemented vitrification solutions

Cited by 8 publications

References 29 publications

Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process

Lipid profile of bovine grade-1 blastocysts produced either in vivo or in vitro before and after slow freezing process

Effects of the donor factors and freezing protocols on the bovine embryonic lipid profile

O impacto da estimulação ovariana, fertilização in vitro, vitrificação de oócitos e suplementação dos meios de vitrificação com L-carnitina e ácidos graxos no perfil lipídico de oócitos e blastocistos murinos

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