Lipidomic profiles as a tool to search for new biomarkers

Pisarska, Anna; Wąsowicz, Wojciech; Gromadzińska, Jolanta

doi:10.13075/ijomeh.1896.01857

Cited by 9 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lipids play one of the most significant roles in any living organism, facilitating energy metabolism and signaling processes. Lipidomics profiling is widely applied in studies of diseases based on inborn metabolic errors [ 44 ]. The methods of lipidomics enable the identification of specific lipid classes that accumulate in the body during these diseases, which could later be used as diagnostic targets.…”

Section: Research Field Landscapementioning

confidence: 99%

Advances in Mass Spectrometry-Based Blood Metabolomics Profiling for Non-Cancer Diseases: A Comprehensive Review

Demicheva,

Dordiuk,

Polanco Espino

et al. 2024

Metabolites

View full text Add to dashboard Cite

Blood metabolomics profiling using mass spectrometry has emerged as a powerful approach for investigating non-cancer diseases and understanding their underlying metabolic alterations. Blood, as a readily accessible physiological fluid, contains a diverse repertoire of metabolites derived from various physiological systems. Mass spectrometry offers a universal and precise analytical platform for the comprehensive analysis of blood metabolites, encompassing proteins, lipids, peptides, glycans, and immunoglobulins. In this comprehensive review, we present an overview of the research landscape in mass spectrometry-based blood metabolomics profiling. While the field of metabolomics research is primarily focused on cancer, this review specifically highlights studies related to non-cancer diseases, aiming to bring attention to valuable research that often remains overshadowed. Employing natural language processing methods, we processed 507 articles to provide insights into the application of metabolomic studies for specific diseases and physiological systems. The review encompasses a wide range of non-cancer diseases, with emphasis on cardiovascular disease, reproductive disease, diabetes, inflammation, and immunodeficiency states. By analyzing blood samples, researchers gain valuable insights into the metabolic perturbations associated with these diseases, potentially leading to the identification of novel biomarkers and the development of personalized therapeutic approaches. Furthermore, we provide a comprehensive overview of various mass spectrometry approaches utilized in blood metabolomics research, including GC-MS, LC-MS, and others discussing their advantages and limitations. To enhance the scope, we propose including recent review articles supporting the applicability of GC×GC-MS for metabolomics-based studies. This addition will contribute to a more exhaustive understanding of the available analytical techniques. The Integration of mass spectrometry-based blood profiling into clinical practice holds promise for improving disease diagnosis, treatment monitoring, and patient outcomes. By unraveling the complex metabolic alterations associated with non-cancer diseases, researchers and healthcare professionals can pave the way for precision medicine and personalized therapeutic interventions. Continuous advancements in mass spectrometry technology and data analysis methods will further enhance the potential of blood metabolomics profiling in non-cancer diseases, facilitating its translation from the laboratory to routine clinical application.

show abstract

Section: Research Field Landscapementioning

confidence: 99%

Advances in Mass Spectrometry-Based Blood Metabolomics Profiling for Non-Cancer Diseases: A Comprehensive Review

Demicheva,

Dordiuk,

Polanco Espino

et al. 2024

Metabolites

View full text Add to dashboard Cite

show abstract

“…Lipids and lipid metabolites in blood and saliva have been used as markers of hazardous chemical exposure 101,102 . The tear uid lipidome may also retain identi able changes associated with environmental exposures.…”

Section: Biological Markers Of Exposure In the Tear Fluidmentioning

confidence: 99%

Tear Fluid as a Matrix for Biomonitoring Environmental and Chemical Exposures

Amini,

Okeme

2023

Preprint

View full text Add to dashboard Cite

Purpose: Exposures to hazardous chemicals have been linked to many detrimental health effects. It is critical to have effective biomonitoring methods to measure the levels of these chemicals in humans to better evaluate key environmental exposures that increase the risk of chronic disease and death. Traditional biomonitoring utilizing blood and urine is limited due to the specialized skills and invasiveness of collecting these fluid samples. The systematic review focuses on the tear fluid, which is largely under-researched, as promising alternative to these traditional fluids. The objective is to evaluate the practicability of using human tear fluid for biomonitoring environmental exposures, highlighting potential pitfalls and opportunities. Recent finding: Tear fluid biomonitoring represents a promising method for assessing exposures. Tear fluid uniquely interfaces with the external environment at the air-tear interface, providing a surface for airborne chemicals to diffuse into the ocular environment and interact with biomolecules. Tear fluid also contains molecules from the internal environment that have travelled from the blood to tears by crossing the blood-tear barrier. Our review shows that tear fluid can be used to identify hazardous chemicals from the external and environment environments and to differentiate between different exposure groups. Summary: The review provides a critical analysis of the current state of knowledge in tear-based biomonitoring and draws attention to the promise of using the method for noninvasively increasing access to exposure assessment. The method will become acceptable for routine use as more individual and inter lab studies focus on broadly identifying and quantify exposure markers in tears, understanding factors that influence reliability of the method and standardizing the tear fluid method.

show abstract

“…Hence, in this study, we investigate the time-dependent changes of lipid profiles in various murine tissue homogenates, i.e., liver, kidney, heart, and spleen homogenates that were stored at RT as well as in ice water (IW). Liver, kidney, heart, and spleen samples were chosen as they all represent tissue types with high metabolic activity [18][19][20][21] as well as (patho-)physiological relevance [3] and are also found in numerous studies [22][23][24][25]. We opted for 25% ethanol in water with the addition of 10 µM indometacin as extraction solvent since we found 25% ethanol a compromise between lipid solubility and avoiding protein precipitation.…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Storage Conditions on Lipid Stability in Mice Tissue Homogenates

et al. 2023

View full text Add to dashboard Cite

Lipids are biomolecules involved in numerous (patho-)physiological processes and their elucidation in tissue samples is of particular interest. However, tissue analysis goes hand in hand with many challenges and the influence of pre-analytical factors can intensively change lipid concentrations ex vivo, compromising the results of the whole research project. Here, we study the influence of pre-analytical factors on lipid profiles during the processing of homogenized tissues. Homogenates from four different mice tissues (liver, kidney, heart, spleen) were stored at room temperature as well as in ice water for up to 120 min and analyzed via ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS). Lipid class ratios were calculated since their suitability as indicators for sample stability has been previously illustrated. Only approx. 40% of lipid class ratios were unchanged after 35 min, which was further reduced to 25% after 120 min during storage at room temperature. In contrast, lipids in tissue homogenates were generally stable when samples were kept in ice water, as more than 90% of investigated lipid class ratios remained unchanged after 35 min. Ultimately, swift processing of tissue homogenates under cooled conditions represents a viable option for lipid analysis and pre-analytical factors require more attention to achieve reliable results.

show abstract

Lipidomic profiles as a tool to search for new biomarkers

Cited by 9 publications

References 35 publications

Advances in Mass Spectrometry-Based Blood Metabolomics Profiling for Non-Cancer Diseases: A Comprehensive Review

Advances in Mass Spectrometry-Based Blood Metabolomics Profiling for Non-Cancer Diseases: A Comprehensive Review

Tear Fluid as a Matrix for Biomonitoring Environmental and Chemical Exposures

Effects of Different Storage Conditions on Lipid Stability in Mice Tissue Homogenates

Contact Info

Product

Resources

About