Lipidomics and Transcriptomics in Neurological Diseases

Post, Julia M.; Lerner, Robert G.; Schwitter, Claudia; Lutz, Beat; Lomazzo, Ermelinda; Bîndilă, Laura

doi:10.3791/59423

Cited by 8 publications

(8 citation statements)

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“…Lipid extraction and lipidomic analyses were performed using established methods as published before ( 26 ). Because of financial and practical reasons, we had to limit the number of metabolites.…”

Section: Methodsmentioning

confidence: 99%

“…( 26 , 29 ) and subsequently extracted using methyl-tert-butyl-ether-based liquid-liquid extraction protocols for PLs and eicosanoids, respectively ( 30 ). Selected PLs and eicosanoid species were analyzed using liquid-chromatography multiple reaction monitoring (MRM) on a QTRAP 5500 (AB Sciex, Darmstadt, Germany) operating in positive negative ion mode switching, using analytical conditions described ( 26 , 29 ). For targeted quantification, MRM transitions of the molecular ions to class-specific ion fragments were used.…”

Section: Methodsmentioning

confidence: 99%

“…For PL analysis also to fatty acyl chain ion fragments to dissect the peaks corresponding to the targeted PL composition. Aqueous phase resulting from liquid-liquid extraction lipid extraction was used for protein content determination of the tissues using a BCA assay (FLUOstar instrument) ( 26 , 31 ). The lipid values determined by LC/MRM were normalized to tissue protein content.…”

Section: Methodsmentioning

confidence: 99%

“…Lipid extraction and lipidomic analyses were performed using established methods as published before (64).…”

Section: Kidney Cortex Lipidomicsmentioning

confidence: 99%

“…Briefly, kidney cortex tissue samples (P39) for both PLs and eicosanoids analysis were spiked with internal standards (Supplemental Table 3). The internal standards were used according to Post et al 2022 andLerner et al 2019 (40, 64) and subsequently extracted using methyl-tert-butyl-ether (MTBE)-based liquid-liquid extraction protocols (LLE) for PLs and eicosanoids, respectively (33). Selected PLs and eicosanoid species (see Table 3, Figure 3 and 4) were analyzed using liquid-chromatography multiple reaction monitoring on a QTRAP 5500 (AB Sciex, Darmstadt, Germany) operating in positive negative ion mode switching, using analytical conditions described in (40,64).…”

Section: Kidney Cortex Lipidomicsmentioning

confidence: 99%

See 4 more Smart Citations

Elevated n-3/n-6 PUFA ratio in early life diet reverses adverse intrauterine kidney programming in female rats

Voggel¹,

Fink²,

Zelck³

et al. 2022

Journal of Lipid Research

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Lipid extraction and lipidomic analyses were performed using established methods as published before (64).…”

Section: Kidney Cortex Lipidomicsmentioning

confidence: 99%

Section: Kidney Cortex Lipidomicsmentioning

confidence: 99%

See 3 more Smart Citations

Elevated n-3/n-6 PUFA ratio in early life diet reverses adverse intrauterine kidney programming in female rats

Voggel¹,

Fink²,

Zelck³

et al. 2022

Journal of Lipid Research

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Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues

Schwitter

Lutz

Bîndilă

2022

Methods in Molecular Biology

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NAPE-PLD deletion in stress-TRAPed neurons results in an anxiogenic phenotype

et al. 2023

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Anandamide (AEA) is an endogenous ligand of the cannabinoid CB1 and CB2 receptors, being a component of the endocannabinoid signaling system, which supports the maintenance or regaining of neural homeostasis upon internal and external challenges. AEA is thought to play a protective role against the development of pathological states after prolonged stress exposure, including depression and generalized anxiety disorder. Here, we used the chronic social defeat (CSD) stress as an ethologically valid model of chronic stress in male mice. We characterized a genetically modified mouse line where AEA signaling was reduced by deletion of the gene encoding the AEA synthesizing enzyme N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D (NAPE-PLD) specifically in neurons activated at the time of CSD stress. One week after the stress, the phenotype was assessed in behavioral tests and by molecular analyses. We found that NAPE-PLD deficiency in neurons activated during the last three days of CSD stress led to an increased anxiety-like behavior. Investigating the molecular mechanisms underlying this phenotype may suggest three main altered pathways to be affected: (i) desensitization of the negative feedback loop of the hypothalamic-pituitary-adrenal axis, (ii) disinhibition of the amygdala by the prefrontal cortex, and (iii) altered neuroplasticity in the hippocampus and prefrontal cortex.

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Lipidomics and Transcriptomics in Neurological Diseases

Cited by 8 publications

References 0 publications

Elevated n-3/n-6 PUFA ratio in early life diet reverses adverse intrauterine kidney programming in female rats

Elevated n-3/n-6 PUFA ratio in early life diet reverses adverse intrauterine kidney programming in female rats

Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues

NAPE-PLD deletion in stress-TRAPed neurons results in an anxiogenic phenotype

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