Lipids are required for the development of <scp>B</scp>razil nut allergy: the role of mouse and human i<scp>NKT</scp> cells

Mirotti, Luciana; Florsheim, Esther; Rundqvist, Louise; Larsson, Göran; Spinozzi, Fabrizio; Leite‐de‐Moraes, Maria; Russo, Marco; Alcocer, Marcos

doi:10.1111/all.12057

Cited by 47 publications

(72 citation statements)

References 37 publications

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“…Lipids from the pollen coat are codelivered with the allergens and can modulate the immune responses of predisposed subjects by interacting with the innate immune system and iiNKT cells, and they can potentially induce NKT cell-dependent allergy disease and asthma [7,11,12]. …”

Section: Discussionmentioning

confidence: 99%

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Human Invariant Natural Killer T Cells Respond to Antigen-Presenting Cells Exposed to Lipids from Olea europaea Pollen

Gracia

López-Relaño²,

Revilla³

et al. 2017

Int Arch Allergy Immunol

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Background: Allergic sensitization might be influenced by the lipids present in allergens, which can be recognized by natural killer T (NKT) cells on antigen-presenting cells (APCs). The aim of this study wasto analyze the effect of olive pollen lipids in human APCs, including monocytes as well as monocyte-derived macrophages (Mϕ) and dendritic cells (DCs). Methods: Lipids were extracted from olive (Olea europaea) pollen grains. Invariant (i)NKT cells, monocytes, Mϕ, and DCs were obtained from buffy coats of healthy blood donors, and their cell phenotype was determined by flow cytometry. iNKT cytotoxicity was measured using a lactate dehydrogenase assay. Gene expression of CD1A and CD1D was performed by RT-PCR, and the production of IL-6, IL-10, IL-12, and TNF-α cytokines by monocytes, Mϕ, and DCs was measured by ELISA. Results: Our results showed that monocytes and monocyte-derived Mϕ treated with olive pollen lipids strongly activate iNKT cells. We observed several phenotypic modifications in the APCs upon exposure to pollen-derived lipids. Both Mϕ and monocytes treated with olive pollen lipids showed an increase in CD1D gene expression, whereas upregulation of cell surface CD1d protein occurred only in Mϕ. Furthermore, DCs differentiated in the presence of human serum enhance their surface CD1d expression when exposed to olive pollen lipids. Finally, olive pollen lipids were able to stimulate the production of IL-6 but downregulated the production of lipopolysaccharide- induced IL-10 by Mϕ. Conclusions: Olive pollen lipids alter the phenotype of monocytes, Mϕ, and DCs, resulting in the activation of NKT cells, which have the potential to influence allergic immune responses.

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Section: Discussionmentioning

confidence: 99%

“…For example, an increased frequency of bee and wasp venom responsive to CD1a-restricted T cells in allergic individuals was recently described [31]. Furthermore, endogenous lipids from Brazil nut can act as adjuvants for the immune response against Ber e 1 allergen and iNKT cells play a crucial role in this response [12]. …”

Section: Discussionmentioning

confidence: 99%

Human Invariant Natural Killer T Cells Respond to Antigen-Presenting Cells Exposed to Lipids from Olea europaea Pollen

Gracia

López-Relaño²,

Revilla³

et al. 2017

Int Arch Allergy Immunol

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“…64 However, it should be noted that some preliminary data suggest that plants and their pollen might contain iNKT cell antigens as well. [83][84][85] Altogether, recent findings indicate that antigens for iNKT cells are almost ubiquitous indoors and in the environment. As they will reach the airways with the inhaled air, it seems likely that iNKT cell antigens are present under normal conditions in the lung at low levels and could under certain conditions contribute to airway inflammation.…”

Section: Inkt Cells and The Microbiota In The Respiratory Tractmentioning

confidence: 99%

The interaction between invariant Natural Killer T cells and the mucosal microbiota

Hapil¹,

Wingender

2018

Immunology

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SummaryThe surface of mammalian bodies is colonized by a multitude of microbial organisms, which under normal conditions support the host and are considered beneficial commensals. This requires, however, that the composition of the commensal microbiota is tightly controlled and regulated. The host immune system plays an important role in the maintenance of this microbiota composition. Here we focus on the contribution of one particular immune cell type, invariant Natural Killer T (iNKT) cells, in this process. The iNKT cells are a unique subset of T cells characterized by two main features. First, they express an invariant T-cell receptor that recognizes glycolipid antigens presented by CD1d, a non-polymorphic major histocompatibility complex class I-like molecule. Second, iNKT cells develop as effector/memory cells and swiftly exert effector functions, like cytokine production and cytotoxicity, after activation. We outline the influence that the mucosal microbiota can have on iNKT cells, and how iNKT cells contribute to the maintenance of the microbiota composition.

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“…are not iNKT cells (Exley et al 2008;Montoya et al 2007), it is unclear at this time whether iNKT cells can respond to pollen derived lipids. Another study implicated recognition of plant lipids by iNKT cells (Mirotti et al 2013), but this recognition was not formally demonstrated.…”

Section: Further Aspects Of Environmental Inkt Cell Antigensmentioning

confidence: 99%

From the Deep Sea to Everywhere: Environmental Antigens for iNKT Cells

Wingender

2015

Arch. Immunol. Ther. Exp.

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Invariant natural killer T (iNKT) cells are a unique subset of innate T cells that share features with innate NK cells and adaptive memory T cells. The first iNKT cell antigen described was found 1993 in a marine sponge and it took over 10 years for other, bacterial antigens to be described. Given the paucity of known bacterial iNKT cell antigens, it appeared as if iNKT cells play a very specialist role in the protection against few, rare and unusual pathogenic bacteria. However, in the last few years several publications painted a very different picture, suggesting that antigens for iNKT cells are found almost ubiquitous in the environment. These environmental iNKT cell antigens can shape the distribution, phenotype and function of iNKT cells. Here, these recent findings will be reviewed and their implications for the field will be outlined.

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Lipids are required for the development of Brazil nut allergy: the role of mouse and human iNKT cells

Abstract: Lipid fraction C from Brazil nut presents an essential adjuvant activity to Ber e 1 sensitization, and iNKT cells play a critical role in the development of Brazil nut-allergic response.

Cited by 47 publications

References 37 publications

Human Invariant Natural Killer T Cells Respond to Antigen-Presenting Cells Exposed to Lipids from Olea europaea Pollen

Human Invariant Natural Killer T Cells Respond to Antigen-Presenting Cells Exposed to Lipids from Olea europaea Pollen

The interaction between invariant Natural Killer T cells and the mucosal microbiota

From the Deep Sea to Everywhere: Environmental Antigens for iNKT Cells

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