Lipolytic activity toward tri- and monoglycerides in postheparin plasma

Response of Plasma Histaminase Activity to Small Doses of Heparin in Normal Subjects and Patients with Hyperlipoproteinemia

“…These include enzymes with lipolytic activity such as lipoprotein lipase (glycerol-ester hydrolase, EC 3.1.1.3) (1, 2), monoglyceride hydrolase (2,3), and phospholipase (4), as well as diamine oxidase (5,6), an enzyme that catalyzes the deamination of histamine (histaminase activity), and diamines such as putrescine (7). The release of plasma lipolytic activity occurs after the injection of as little as 10 U heparin/kg (2).…”

Abnormal Lipoprotein Lipase in Familial Exogenous Hypertriglyceridemia

“…Heparin releases at least three other enzyme activities into the circulation: monoglyceridase (26,27), phospholipase (7), and a nonspecific esterase (26,27). We have measured the first two enzymes and found monoglyceridase activity to be normal or low normal in all patients with familial or acquired hyperchylomicronemia.…”

“…Our findings suggest that these are distinct enzymes but do not exclude a mutant lipoprotein lipase with normal affinity for monoolein or phosphatidyl ethanolamine micelles but decreased activity for chylomicron triglyceride. Other workers have previously reported the varying characteristics of the postheparin lipases (7,(27)(28)(29) (31). Perhaps the simplest explanation for these data is that lipoprotein lipase has activity against each of these glycerides but other separate heparin-released lipases also exist.…”

A biochemical and morphologic study of very low density lipoproteins in carbohydrate-induced hypertriglyceridemia

“…In both groups of subjects, the increase in VLDL protein during carbohydrate induction was associated with a significant decrease in the protein content of 1.006 < d < .S_ I1F 12 14 16 18 Days of study FIGURE 3 Relation between plasma triglyceride levels (0), mean particle diameter (A) and the triglyceride/protein ratio (0) of d < 1.006 lipoprotein in two hypertriglyceridemic subjects on a high carbohydrate diet and during a fast.…”

“…Four hypertriglyceridemic patients with normal glucose tolerance and normal levels of plasma postheparin lipolytic activity (14) were also investigated (Table I) identical with that of the control group, except that their induction period was started after a 36 hr fast to minimize the presence of chylomicra in the circulation. Two of the hypertriglyceridemic subjects (P. D. and E. L.) were induced while on a metabolic ward, and the remainder as outpatients.…”