Lipopeptides Produced by a Soil Bacillus Megaterium Strain

Pueyo, Manuel Troyano; Bloch, Carlos; Carmona-Ribeiro, Ana Maria; Mascio, Paolo Di

doi:10.1007/s00248-008-9464-x

Cited by 75 publications

(62 citation statements)

References 47 publications

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“…The IR spectrum obtained from the ultrafiltered products showed close resemblance to previously reported lipopeptides spectrum previously, as in the case of fengycin from B. circulans DMS-2 [4,13] and lipopeptides from B. subtilis [15]. IR spectrum of also showed a close resemblance of surfactin, lichenysins, fengycins from B. magaterium [17].…”

Section: Ftirsupporting

confidence: 84%

“…Most of the Bacillus sp. has been reported to produce both groups of lipopeptides in same medium during its metabolic pathway [11,17]. The production of different groups of lipopeptides by the same microorganism has been established in previous reports [11,17,20].…”

Section: Maldi-tofmentioning

confidence: 80%

“…2). The IR pattern of the both the products revealed the presence of lipopeptide groups present in the produced molecules [13][14][15][16][17]. In spectrum A (UF-I product), major peaks were observed in transmittance mode at the range 3,270, 2,923, 1,650, 1,542 cm -1 and some minor peaks were observed at 3,062, 2,962, 2,850, 1,454 and 1,234 cm -1 .…”

Section: Ftirmentioning

confidence: 99%

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Single step concomitant concentration, purification and characterization of two families of lipopeptides of marine origin

Sivapathasekaran

Mukherjee

Sen

et al. 2010

Bioprocess Biosyst Eng

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The extracellular biosurfactant product secreted by a marine bacterium was concentrated and purified directly from the fermentation broth in a single step by ultrafiltration (UF) employing YM 30 kDa (UF-I) and Omega 10 kDa (UF-II) polyethersulfone membranes. The optimum operating pressure required for both membranes, UF-I and UF-II, were found to be 30 and 35 psi, respectively. The biosurfactant from the fermentation broth was recovered in higher amounts using UF-II (89%) than using UF-I (73%). An analysis of the critical micelle concentrations (CMC) of the recovered lipopeptides showed a lower CMC value of 15 mg L⁻¹ for the UF-II product, indicating higher degree of purity (83%) when compared to that of the UF-I product (78%). The ultrafiltered products were characterized using Fourier transformed infrared spectroscopy and matrix-assisted laser desorption ionization time of flight mass spectral analysis, which demonstrated the presence of two families of lipopeptides.

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Section: Ftirsupporting

confidence: 84%

Section: Maldi-tofmentioning

confidence: 80%

Section: Ftirmentioning

confidence: 99%

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Single step concomitant concentration, purification and characterization of two families of lipopeptides of marine origin

Sivapathasekaran

Mukherjee

Sen

et al. 2010

Bioprocess Biosyst Eng

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“…However, biosurfactants have not yet been employed extensively in industry because their production is not optimized [1,30]. Among the reasons they have not yet been commercialized extensively is the high costs of feedstocks [10,21,25].…”

Section: Introductionmentioning

confidence: 99%

Purification and structural characterization of fengycin homologues produced by Bacillus subtilis LSFM-05 grown on raw glycerol

Faria

Stéfani

Vaz

et al. 2011

J Ind Microbiol Biotechnol

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Raw glycerol is a byproduct of biodiesel production that currently has low to negative value for biodiesel producers. One option for increasing the value of raw glycerol is to use it as a feedstock for microbial production. Bacillus subtilis LSFM 05 was used for the production of fengycin in a mineral medium containing raw glycerol as the sole carbon source. Fengycin was isolated by acid precipitation at pH 2 and purified by silica gel column chromatography and characterized using electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS) with collision-induced dissociation (CID). The mass spectrum revealed the presence of the ions of m/z 1,435.7, 1,449.9, 1,463.8, 1,477.8, 1,491.8 and 1,505.8, which were further fragmented by ESI-MS/MS. The CID profile showed the presence of a series of ions (m/z 1,080 and 966) and (m/z 1,108 and 994) that represented the different fengycin homologues A and B, respectively. Fengycin homologues A and B are variants that differ at position 6 of the peptide moiety, having either Ala or Val residues, respectively. Mass spectrometry analyses identified four fengycin A and three fengycin B variants with fatty acid components containing 14-17 carbons. These results demonstrate that raw glycerol can be used as feedstock to produce fengycin, and additional work should focus on the optimization of process conditions to increase productivity.

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“…The mechanism of fengycin action was revealed as a two-state transition controlled by the lipopeptide concentration -one state being monomeric, not deeply anchored and nonperturbing lipopeptide and other burried, aggregated form responsible for membrane leakage and bioactivity (Deleu et al, 2008). A soil microorganism identified as Bacillus megaterium was found to produce several antibiotics (Pueyo et al, 2009). Analysis both by electron spray ionization (ESI) and matrixassisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) identified these substances as lipopeptides.…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%