Lipopeptides Produced by Bacillus mojavensis P1709 as an Efficient Tool to Maintain Postharvest Cherry Tomato Quality and Quantity

Galitskaya, Polina; Karamova, Kamalya; Biktasheva, Liliya; Galieva, Gulnaz; Gordeev, Alexander; Selivanovskaya, Svetlana

doi:10.3390/agriculture12050609

Cited by 14 publications

(12 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study suggested that the LPs from B. subtilis ABS-S14 act as defense-related genes to inhibit pathogen growth (Waewthongrak et al, 2014). Another study produced similar results showing that LPs produced by B. mojavensis P1709 are an effective tool for controlling postharvest decay and mycotoxin contamination in cherry tomatoes (Galitskaya et al, 2022). In conclusion, we report that LPs produced by B. subtilis strain Y17B potentially exert strong antifungal activity against A. alternata, causing ultrastructural damage to the pathogen and reducing the disease intensity of A. alternata on cherry fruit.…”

Section: Discussionmentioning

confidence: 83%

Biocontrol potential of lipopeptides produced by the novel Bacillus subtilis strain Y17B against postharvest Alternaria fruit rot of cherry

et al. 2023

View full text Add to dashboard Cite

The use of synthetic fungicides against postharvest Alternaria rot adversely affects human health and the environment. In this study, as a safe alternative to fungicides, Bacillus subtilis strain Y17B isolated from soil exhibited significant antifungal activity against Alternaria alternata. Y17B was identified as B. subtilis based on phenotypic identification and 16S rRNA sequence analysis. To reveal the antimicrobial activity of this strain, a PCR-based study detected the presence of antifungal lipopeptide (LP) biosynthetic genes from genomic DNA. UPLC Q TOF mass spectrometry analysis detected the LPs surfactin (m/z 994.64, 1022.68, and 1026.62), iturin (m/z 1043.56), and fengycin (m/z 1491.85) in the extracted LP crude of B. subtilis Y17B. In vitro antagonistic study demonstrated the efficiency of LPs in inhibiting A. alternata growth. Microscopy (SEM and TEM) studies showed the alteration of the morphology of A. alternata in the interaction with LPs. In vivo test results revealed the efficiency of LPs in reducing the growth of the A. alternata pathogen. The overall results highlight the biocontrol potential of LPs produced by B. subtilis Y17B as an effective biological control agent against A. alternata fruit rot of cherry.

show abstract

Section: Discussionmentioning

confidence: 83%

Biocontrol potential of lipopeptides produced by the novel Bacillus subtilis strain Y17B against postharvest Alternaria fruit rot of cherry

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Previous studies have shown that B. mojavensis had inhibitory effects on a variety of pathogens. Galitskaya et al [ 45 ] reported that B. mojavensis P1709 could protect postharvest cherry tomatoes from fungal pathogens, and Bacon et al [ 46 ] reported that Bacillus mojavensis could reduce stalk lesions in maize seedlings. In this study, B. mojavensis BQ-33 had an inhibitory activity on D. glomerata , Botryosphaeria dothidea , Alternaria alternata , Fusarium oxysporum and Phomopsis cauloides .…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activities of Bacillus mojavensis BQ-33 towards the Kiwifruit Black Spot Disease Caused by the Fungal Pathogen Didymella glomerata

et al. 2022

View full text Add to dashboard Cite

‘Hongyang’ kiwifruit (Actinidia chinensis, cultivar ‘Hongyang’) black spot disease is caused by the fungal pathogen Didymella glomerata, and is a serious disease, causing considerable losses to the kiwifruit industry during growth of the fruit. Hence, we aimed to identify a potential biocontrol agent against D. glomerata. In this study, bacterial isolates from the rhizosphere soil of kiwifruit were tested for their potential antifungal activity against selected fungal pathogens. Based on a phylogenetic tree constructed using sequences of 16S rDNA and the gyrA gene, BQ-33 with the best antifungal activity was identified as Bacillus mojavensis. We evaluated the antagonistic activity and inhibitory mechanism of BQ-33 against D. glomerata. Confrontation experiments showed that both BQ-33 suspension and the sterile supernatant (SS) produced by BQ-33 possessed excellent broad-spectrum antifungal activity. Furthermore, the SS damaged the cell membrane and cell wall of the mycelia, resulting in the leakage of a large quantity of small ions (Na+, K+), soluble proteins and nucleic acids. Chitinase and β-1,3-glucanase activities in SS increased in correlation with incubation time and remained at a high level for several days. An in vivo control efficacy assay indicated that 400 mL L−1 of SS completely inhibited kiwifruit black spot disease caused by D. glomerata. Therefore, BQ-33 is a potential biocontrol agent against kiwifruit black spot and plant diseases caused by other fungal pathogens. To our knowledge, this is the first report of the use of a rhizosphere microorganism as a biocontrol agent against kiwifruit black spot disease caused by D. glomerata.

show abstract

“…Because of significant yield losses caused by fungal pathogens, new, efficient, and environmentally safe methods of pest control are needed, hence, amphiphilic compounds or the BSs produced by many microbes are considered a good alternative. Galitskaya et al (2022) reported the use of B. mojavensis P1709-derived lipopeptide BSs against F. oxysporum f. sp. lycopersici affecting the cherry tomato.…”

Section: Use Of Biosurfactants And/or Bs-producing Microorganisms In ...mentioning

confidence: 99%

“… Galitskaya et al (2022) reported the use of B. mojavensis P1709-derived lipopeptide BSs against F. oxysporum f. sp. lycopersici affecting the cherry tomato.…”

Section: Use Of Biosurfactants And/or Bs-producing Microorganisms In ...mentioning

confidence: 99%

Biosurfactants’ multifarious functional potential for sustainable agricultural practices

Karamchandani

Pawar

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Increasing food demand by the ever-growing population imposes an extra burden on the agricultural and food industries. Chemical-based pesticides, fungicides, fertilizers, and high-breeding crop varieties are typically employed to enhance crop productivity. Overexploitation of chemicals and their persistence in the environment, however, has detrimental effects on soil, water, and air which consequently disturb the food chain and the ecosystem. The lower aqueous solubility and higher hydrophobicity of agrochemicals, pesticides, metals, and hydrocarbons allow them to adhere to soil particles and, therefore, continue in the environment. Chemical pesticides, viz., organophosphate, organochlorine, and carbamate, are used regularly to protect agriculture produce. Hydrophobic pollutants strongly adhered to soil particles can be solubilized or desorbed through the usage of biosurfactant/s (BSs) or BS-producing and pesticide-degrading microorganisms. Among different types of BSs, rhamnolipids (RL), surfactin, mannosylerythritol lipids (MELs), and sophorolipids (SL) have been explored extensively due to their broad-spectrum antimicrobial activities against several phytopathogens. Different isoforms of lipopeptide, viz., iturin, fengycin, and surfactin, have also been reported against phytopathogens. The key role of BSs in designing and developing biopesticide formulations is to protect crops and our environment. Various functional properties such as wetting, spreading, penetration ability, and retention period are improved in surfactant-based formulations. This review emphasizes the use of diverse types of BSs and their source microorganisms to challenge phytopathogens. Extensive efforts seem to be focused on discovering the innovative antimicrobial potential of BSs to combat phytopathogens. We discussed the effectiveness of BSs in solubilizing pesticides to reduce their toxicity and contamination effects in the soil environment. Thus, we have shed some light on the use of BSs as an alternative to chemical pesticides and other agrochemicals as sparse literature discusses their interactions with pesticides. Life cycle assessment (LCA) and life cycle sustainability analysis (LCSA) quantifying their impact on human activities/interventions are also included. Nanoencapsulation of pesticide formulations is an innovative approach in minimizing pesticide doses and ultimately reducing their direct exposures to humans and animals. Some of the established big players and new entrants in the global BS market are providing promising solutions for agricultural practices. In conclusion, a better understanding of the role of BSs in pesticide solubilization and/or degradation by microorganisms represents a valuable approach to reducing their negative impact and maintaining sustainable agricultural practices.

show abstract

Lipopeptides Produced by Bacillus mojavensis P1709 as an Efficient Tool to Maintain Postharvest Cherry Tomato Quality and Quantity

Cited by 14 publications

References 57 publications

Biocontrol potential of lipopeptides produced by the novel Bacillus subtilis strain Y17B against postharvest Alternaria fruit rot of cherry

Biocontrol potential of lipopeptides produced by the novel Bacillus subtilis strain Y17B against postharvest Alternaria fruit rot of cherry

Antifungal Activities of Bacillus mojavensis BQ-33 towards the Kiwifruit Black Spot Disease Caused by the Fungal Pathogen Didymella glomerata

Biosurfactants’ multifarious functional potential for sustainable agricultural practices

Contact Info

Product

Resources

About